Formylation Is Not Essential for Initiation of Protein Synthesis in All Eubacteria
Formylation of the initiator methionyl-tRNA, catalyzed by methionyl-tRNA formyltransferase, has long been regarded as essential for initiation of protein synthesis in eubacteria. Here, we show that this process is, in fact, dispensable in Pseudomonas aeruginosa . Disruption of the chromosomal methio...
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| Veröffentlicht in: | The Journal of biological chemistry 1999-08, Vol.274 (32), p.22143-22146 |
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| container_issue | 32 |
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| container_title | The Journal of biological chemistry |
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| creator | Newton, D T Creuzenet, C Mangroo, D |
| description | Formylation of the initiator methionyl-tRNA, catalyzed by methionyl-tRNA formyltransferase, has long been regarded as essential
for initiation of protein synthesis in eubacteria. Here, we show that this process is, in fact, dispensable in Pseudomonas aeruginosa . Disruption of the chromosomal methionyl-tRNA formyltransferase gene in P. aeruginosa resulted only in a moderate decrease in the rate of cell growth, whereas in Escherichia coli cell growth was severely impaired. The ability of the P. aeruginosa mutant strain to grow was not due to an additional copy of the methionyl-tRNA formyltransferase gene or to N -acylation of the methionyl moiety by a group other than formyl. These results indicate that P. aeruginosa can carry out formylation-independent initiation of protein synthesis, using the nonformylated methionyl-tRNA. Therefore,
the dogma that eubacteria require formylation of the initiator methionyl-tRNA for initiation of protein synthesis may have
been an invalid generalization of results obtained with E. coli . |
| doi_str_mv | 10.1074/jbc.274.32.22143 |
| format | Article |
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for initiation of protein synthesis in eubacteria. Here, we show that this process is, in fact, dispensable in Pseudomonas aeruginosa . Disruption of the chromosomal methionyl-tRNA formyltransferase gene in P. aeruginosa resulted only in a moderate decrease in the rate of cell growth, whereas in Escherichia coli cell growth was severely impaired. The ability of the P. aeruginosa mutant strain to grow was not due to an additional copy of the methionyl-tRNA formyltransferase gene or to N -acylation of the methionyl moiety by a group other than formyl. These results indicate that P. aeruginosa can carry out formylation-independent initiation of protein synthesis, using the nonformylated methionyl-tRNA. Therefore,
the dogma that eubacteria require formylation of the initiator methionyl-tRNA for initiation of protein synthesis may have
been an invalid generalization of results obtained with E. coli .</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.274.32.22143</identifier><identifier>PMID: 10428776</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Acylation ; Base Sequence ; Escherichia coli ; Escherichia coli - enzymology ; Eubacteria ; Eubacterium ; Genes, Bacterial ; Hydroxymethyl and Formyl Transferases - genetics ; Hydroxymethyl and Formyl Transferases - metabolism ; Molecular Sequence Data ; Mutation ; Nucleic Acid Conformation ; Peptide Chain Initiation, Translational ; Phenotype ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - enzymology ; RNA, Transfer, Met - metabolism</subject><ispartof>The Journal of biological chemistry, 1999-08, Vol.274 (32), p.22143-22146</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-cc9d1ce52bf4088a0c861216537e1745d8faf3e7e52c667a2028d539de3502733</citedby><cites>FETCH-LOGICAL-c397t-cc9d1ce52bf4088a0c861216537e1745d8faf3e7e52c667a2028d539de3502733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10428776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Newton, D T</creatorcontrib><creatorcontrib>Creuzenet, C</creatorcontrib><creatorcontrib>Mangroo, D</creatorcontrib><title>Formylation Is Not Essential for Initiation of Protein Synthesis in All Eubacteria</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Formylation of the initiator methionyl-tRNA, catalyzed by methionyl-tRNA formyltransferase, has long been regarded as essential
for initiation of protein synthesis in eubacteria. Here, we show that this process is, in fact, dispensable in Pseudomonas aeruginosa . Disruption of the chromosomal methionyl-tRNA formyltransferase gene in P. aeruginosa resulted only in a moderate decrease in the rate of cell growth, whereas in Escherichia coli cell growth was severely impaired. The ability of the P. aeruginosa mutant strain to grow was not due to an additional copy of the methionyl-tRNA formyltransferase gene or to N -acylation of the methionyl moiety by a group other than formyl. These results indicate that P. aeruginosa can carry out formylation-independent initiation of protein synthesis, using the nonformylated methionyl-tRNA. Therefore,
the dogma that eubacteria require formylation of the initiator methionyl-tRNA for initiation of protein synthesis may have
been an invalid generalization of results obtained with E. coli .</description><subject>Acylation</subject><subject>Base Sequence</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Eubacteria</subject><subject>Eubacterium</subject><subject>Genes, Bacterial</subject><subject>Hydroxymethyl and Formyl Transferases - genetics</subject><subject>Hydroxymethyl and Formyl Transferases - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleic Acid Conformation</subject><subject>Peptide Chain Initiation, Translational</subject><subject>Phenotype</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - enzymology</subject><subject>RNA, Transfer, Met - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqWwMyEPiC3BH0nsjFXVQqUKEB8Sm-U4DnWVxMVOhPrvMU0HmLjl7nTPvcMDwCVGMUYsud0UKiYsiSmJCcEJPQJjjDiNaIrfj8EYIYKjnKR8BM6836BQSY5PwQijhHDGsjF4XljX7GrZGdvCpYcPtoNz73XbGVnDyjq4bE2Y93dbwSdnO21a-LJru7X2xsOwTOsazvtCqk47I8_BSSVrry8OfQLeFvPX2X20erxbzqarSNGcdZFSeYmVTklRJYhziRTPMMFZSpnGLElLXsmKahYIlWVMEkR4mdK81DRFhFE6ATdD7tbZz177TjTGK13XstW29yLLc5JTjv4FMQtxQUwA0QAqZ713uhJbZxrpdgIj8SNcBOEiCBeUiL3w8HJ1yO6LRpe_HgbDAbgegLX5WH8Zp0VhrFrr5m_ON3f1hvI</recordid><startdate>19990806</startdate><enddate>19990806</enddate><creator>Newton, D T</creator><creator>Creuzenet, C</creator><creator>Mangroo, D</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19990806</creationdate><title>Formylation Is Not Essential for Initiation of Protein Synthesis in All Eubacteria</title><author>Newton, D T ; Creuzenet, C ; Mangroo, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-cc9d1ce52bf4088a0c861216537e1745d8faf3e7e52c667a2028d539de3502733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acylation</topic><topic>Base Sequence</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Eubacteria</topic><topic>Eubacterium</topic><topic>Genes, Bacterial</topic><topic>Hydroxymethyl and Formyl Transferases - genetics</topic><topic>Hydroxymethyl and Formyl Transferases - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleic Acid Conformation</topic><topic>Peptide Chain Initiation, Translational</topic><topic>Phenotype</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - enzymology</topic><topic>RNA, Transfer, Met - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Newton, D T</creatorcontrib><creatorcontrib>Creuzenet, C</creatorcontrib><creatorcontrib>Mangroo, D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Newton, D T</au><au>Creuzenet, C</au><au>Mangroo, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formylation Is Not Essential for Initiation of Protein Synthesis in All Eubacteria</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1999-08-06</date><risdate>1999</risdate><volume>274</volume><issue>32</issue><spage>22143</spage><epage>22146</epage><pages>22143-22146</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Formylation of the initiator methionyl-tRNA, catalyzed by methionyl-tRNA formyltransferase, has long been regarded as essential
for initiation of protein synthesis in eubacteria. Here, we show that this process is, in fact, dispensable in Pseudomonas aeruginosa . Disruption of the chromosomal methionyl-tRNA formyltransferase gene in P. aeruginosa resulted only in a moderate decrease in the rate of cell growth, whereas in Escherichia coli cell growth was severely impaired. The ability of the P. aeruginosa mutant strain to grow was not due to an additional copy of the methionyl-tRNA formyltransferase gene or to N -acylation of the methionyl moiety by a group other than formyl. These results indicate that P. aeruginosa can carry out formylation-independent initiation of protein synthesis, using the nonformylated methionyl-tRNA. Therefore,
the dogma that eubacteria require formylation of the initiator methionyl-tRNA for initiation of protein synthesis may have
been an invalid generalization of results obtained with E. coli .</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>10428776</pmid><doi>10.1074/jbc.274.32.22143</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acylation Base Sequence Escherichia coli Escherichia coli - enzymology Eubacteria Eubacterium Genes, Bacterial Hydroxymethyl and Formyl Transferases - genetics Hydroxymethyl and Formyl Transferases - metabolism Molecular Sequence Data Mutation Nucleic Acid Conformation Peptide Chain Initiation, Translational Phenotype Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology RNA, Transfer, Met - metabolism |
| title | Formylation Is Not Essential for Initiation of Protein Synthesis in All Eubacteria |
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