In vitro isolation of Neospora caninum from a stillborn calf in the UK

Neospora caninumwas isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed asN. caninumby immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates ofN. caninumand closely related protozoal parasites, using random amplified polymorphicDNApolymerase chain reaction (RAPD-PCR) techniques.