Soil bacterial community shift correlated with change from forest to pasture vegetation in a tropical soil
The change in vegetative cover of a Hawaiian soil from forest to pasture led to significant changes in the composition of the soil bacterial community. DNAs were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (G + C) content, by analysis of abundance of phy...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1999-08, Vol.65 (8), p.3622-3626 |
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| description | The change in vegetative cover of a Hawaiian soil from forest to pasture led to significant changes in the composition of the soil bacterial community. DNAs were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (G + C) content, by analysis of abundance of phylotypes of small-subunit ribosomal DNA (SSU rDNA) amplified from fractions with 63 and 35% G + C contents, and by phylogenetic analysis of the dominant rDNA clones in the 63% G + C content fraction. All three methods showed differences between the forest and pasture habitats, providing evidence that vegetation had a strong influence on microbial community composition at three levels of taxon resolution. The forest soil DNA had a peak in G + C content of 61%, while the DNA of the pasture soil had a peak in G + C content of 67%. None of the dominant phylotypes found in the forest soil were detected in the pasture soil. For the 63% G + C fraction SSU rDNA sequence analysis of the three most dominant members revealed that their phyla changed from Fibrobacter and Syntrophomonas assemblages in the forest soil to Burkholderia and Rhizobium-Agrobacterium assemblages in the pasture soil. |
| doi_str_mv | 10.1128/aem.65.8.3622-3626.1999 |
| format | Article |
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DNAs were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (G + C) content, by analysis of abundance of phylotypes of small-subunit ribosomal DNA (SSU rDNA) amplified from fractions with 63 and 35% G + C contents, and by phylogenetic analysis of the dominant rDNA clones in the 63% G + C content fraction. All three methods showed differences between the forest and pasture habitats, providing evidence that vegetation had a strong influence on microbial community composition at three levels of taxon resolution. The forest soil DNA had a peak in G + C content of 61%, while the DNA of the pasture soil had a peak in G + C content of 67%. None of the dominant phylotypes found in the forest soil were detected in the pasture soil. For the 63% G + C fraction SSU rDNA sequence analysis of the three most dominant members revealed that their phyla changed from Fibrobacter and Syntrophomonas assemblages in the forest soil to Burkholderia and Rhizobium-Agrobacterium assemblages in the pasture soil.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.65.8.3622-3626.1999</identifier><identifier>PMID: 10427058</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Agriculture ; Agrobacterium ; Animal, plant and microbial ecology ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Base Composition ; Biological and medical sciences ; Burkholderia ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Ecosystem ; Fibrobacter ; Fundamental and applied biological sciences. 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DNAs were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (G + C) content, by analysis of abundance of phylotypes of small-subunit ribosomal DNA (SSU rDNA) amplified from fractions with 63 and 35% G + C contents, and by phylogenetic analysis of the dominant rDNA clones in the 63% G + C content fraction. All three methods showed differences between the forest and pasture habitats, providing evidence that vegetation had a strong influence on microbial community composition at three levels of taxon resolution. The forest soil DNA had a peak in G + C content of 61%, while the DNA of the pasture soil had a peak in G + C content of 67%. None of the dominant phylotypes found in the forest soil were detected in the pasture soil. For the 63% G + C fraction SSU rDNA sequence analysis of the three most dominant members revealed that their phyla changed from Fibrobacter and Syntrophomonas assemblages in the forest soil to Burkholderia and Rhizobium-Agrobacterium assemblages in the pasture soil.</description><subject>Agriculture</subject><subject>Agrobacterium</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>Burkholderia</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Ecosystem</subject><subject>Fibrobacter</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>genbank/af165267</topic><topic>genbank/af165268</topic><topic>genbank/af165269</topic><topic>genbank/af165270</topic><topic>genbank/af165271</topic><topic>genbank/af165272</topic><topic>genbank/af165273</topic><topic>genbank/af165274</topic><topic>genbank/af165275</topic><topic>genbank/af165276</topic><topic>genbank/af165277</topic><topic>genbank/af165278</topic><topic>genbank/af165279</topic><topic>genbank/af165280</topic><topic>General Microbial Ecology</topic><topic>Microbial ecology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Rhizobium</topic><topic>ribosomal DNA</topic><topic>Soil</topic><topic>soil bacteria</topic><topic>Soil Microbiology</topic><topic>Syntrophomonas</topic><topic>Trees</topic><topic>Tropical Climate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nusslein, K</creatorcontrib><creatorcontrib>Tiedje, J.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nusslein, K</au><au>Tiedje, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soil bacterial community shift correlated with change from forest to pasture vegetation in a tropical soil</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>65</volume><issue>8</issue><spage>3622</spage><epage>3626</epage><pages>3622-3626</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The change in vegetative cover of a Hawaiian soil from forest to pasture led to significant changes in the composition of the soil bacterial community. DNAs were extracted from both soil habitats and compared for the abundance of guanine-plus-cytosine (G + C) content, by analysis of abundance of phylotypes of small-subunit ribosomal DNA (SSU rDNA) amplified from fractions with 63 and 35% G + C contents, and by phylogenetic analysis of the dominant rDNA clones in the 63% G + C content fraction. All three methods showed differences between the forest and pasture habitats, providing evidence that vegetation had a strong influence on microbial community composition at three levels of taxon resolution. The forest soil DNA had a peak in G + C content of 61%, while the DNA of the pasture soil had a peak in G + C content of 67%. None of the dominant phylotypes found in the forest soil were detected in the pasture soil. For the 63% G + C fraction SSU rDNA sequence analysis of the three most dominant members revealed that their phyla changed from Fibrobacter and Syntrophomonas assemblages in the forest soil to Burkholderia and Rhizobium-Agrobacterium assemblages in the pasture soil.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>10427058</pmid><doi>10.1128/aem.65.8.3622-3626.1999</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1999-08, Vol.65 (8), p.3622-3626 |
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| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Agriculture Agrobacterium Animal, plant and microbial ecology Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Base Composition Biological and medical sciences Burkholderia DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Ecosystem Fibrobacter Fundamental and applied biological sciences. Psychology genbank/af165267 genbank/af165268 genbank/af165269 genbank/af165270 genbank/af165271 genbank/af165272 genbank/af165273 genbank/af165274 genbank/af165275 genbank/af165276 genbank/af165277 genbank/af165278 genbank/af165279 genbank/af165280 General Microbial Ecology Microbial ecology Molecular Sequence Data nucleotide sequences Rhizobium ribosomal DNA Soil soil bacteria Soil Microbiology Syntrophomonas Trees Tropical Climate |
| title | Soil bacterial community shift correlated with change from forest to pasture vegetation in a tropical soil |
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