Protective effect of preconditioning and adenosine pretreatment in experimental skeletal muscle reperfusion injury
Background: Prolonged ischaemia followed by reperfusion (I/R) of skeletal muscle results in significant tissue injury. Ischaemic preconditioning (IPC), achieved by repeated brief periods of I/R before prolonged ischaemia or adenosine pretreatment, can prevent I/R injury in cardiac muscle. The aim of...
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| creator | Papanastasiou, S. Estdale, S. E. Homer-Vanniasinkam, S. Mathie, R. T. |
| description | Background:
Prolonged ischaemia followed by reperfusion (I/R) of skeletal muscle results in significant tissue injury. Ischaemic preconditioning (IPC), achieved by repeated brief periods of I/R before prolonged ischaemia or adenosine pretreatment, can prevent I/R injury in cardiac muscle. The aim of this study was to ascertain in a rodent model if damage to skeletal muscle due to global hindlimb tourniquet‐induced I/R could be similarly attenuated.
Methods:
Anaesthetized rats were randomized (n = 6–10 per group) to five groups: sham‐operated controls; I/R (4 h of ischaemia, 2 h of reperfusion); IPC (three cycles of 10 min of ischaemia/10 min of reperfusion) alone; IPC immediately preceding I/R; or adenosine 1000 µg/kg immediately before I/R. At the end of reperfusion, biopsies were taken from the left gastrocnemius muscle for measurement of myeloperoxidase (MPO) and reduced glutathione (GSH). Before ischaemia and at the end of reperfusion, blood samples were taken for measurement of nitric oxide metabolites, tumour necrosis factor (TNF) α and macrophage inflammatory protein (MIP) 2.
Results:
IPC before I/R resulted in lower levels of MPO (P < 0·001) and TNF‐α (P = 0·004), and higher levels of GSH (P < 0·001) and nitric oxide metabolites (P = 0·002) than I/R alone. Adenosine had effects comparable to IPC pretreatment (P < 0·001 for MPO, P = 0·002 for GSH, P = 0·02 for nitric oxide metabolites and P = 0·001 for TNF‐α). There was no difference in the blood pressure or the MIP‐2 concentration among the groups.
Conclusion:
IPC or pretreatment with adenosine ameliorates the I/R injury of skeletal muscle. © 2000 British Journal of Surgery Society Ltd |
| doi_str_mv | 10.1046/j.1365-2168.1999.01164.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69922326</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69922326</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4983-7e822f6329752c35e02ce8de1499226c25b71dbc66c9cdb72388223c09a9a05b3</originalsourceid><addsrcrecordid>eNpNkc1u1DAURq2Kqh1KXwF5gdgl9U9sxxIbqKC0jKASUJaW49wgTzPO1E5g5u1xmGlh5Wvd8x3J_hDClJSUVPJiVVIuRcGorEuqtS4JpbIqt0do8bR4hhaEEFVQzvgpep7SihDKiWAn6DRLqBJSLFC8jcMIbvS_AEPX5QkPHd5EcENo_eiH4MNPbEOLbQthSD7AvB0j2HENYcQ-YNhuIPr5Znuc7qGHeVhPyfWAI-RlN6VsyuxqirsX6LizfYLzw3mGvn94_-3yY7H8cnV9-XZZuErXvFBQM9ZJzrQSzHEBhDmoW6CV1oxJx0SjaNs4KZ12baMYr3OAO6KttkQ0_Ay93ns3cXiYII1m7ZODvrcBhikZOXs4kxl8eQCnZg2t2eTH2Lgzj7-UgVcHwCZn-y7a4Hz6x9W6qkmVsTd77LfvYfefZjZJszJzN2buxsylmb-lma15d_OVKp7jxT7u0wjbp7iN90YqroT58fnKfFou6Y2q7swt_wN4GJte</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69922326</pqid></control><display><type>article</type><title>Protective effect of preconditioning and adenosine pretreatment in experimental skeletal muscle reperfusion injury</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Papanastasiou, S. ; Estdale, S. E. ; Homer-Vanniasinkam, S. ; Mathie, R. T.</creator><creatorcontrib>Papanastasiou, S. ; Estdale, S. E. ; Homer-Vanniasinkam, S. ; Mathie, R. T.</creatorcontrib><description>Background:
Prolonged ischaemia followed by reperfusion (I/R) of skeletal muscle results in significant tissue injury. Ischaemic preconditioning (IPC), achieved by repeated brief periods of I/R before prolonged ischaemia or adenosine pretreatment, can prevent I/R injury in cardiac muscle. The aim of this study was to ascertain in a rodent model if damage to skeletal muscle due to global hindlimb tourniquet‐induced I/R could be similarly attenuated.
Methods:
Anaesthetized rats were randomized (n = 6–10 per group) to five groups: sham‐operated controls; I/R (4 h of ischaemia, 2 h of reperfusion); IPC (three cycles of 10 min of ischaemia/10 min of reperfusion) alone; IPC immediately preceding I/R; or adenosine 1000 µg/kg immediately before I/R. At the end of reperfusion, biopsies were taken from the left gastrocnemius muscle for measurement of myeloperoxidase (MPO) and reduced glutathione (GSH). Before ischaemia and at the end of reperfusion, blood samples were taken for measurement of nitric oxide metabolites, tumour necrosis factor (TNF) α and macrophage inflammatory protein (MIP) 2.
Results:
IPC before I/R resulted in lower levels of MPO (P < 0·001) and TNF‐α (P = 0·004), and higher levels of GSH (P < 0·001) and nitric oxide metabolites (P = 0·002) than I/R alone. Adenosine had effects comparable to IPC pretreatment (P < 0·001 for MPO, P = 0·002 for GSH, P = 0·02 for nitric oxide metabolites and P = 0·001 for TNF‐α). There was no difference in the blood pressure or the MIP‐2 concentration among the groups.
Conclusion:
IPC or pretreatment with adenosine ameliorates the I/R injury of skeletal muscle. © 2000 British Journal of Surgery Society Ltd</description><identifier>ISSN: 0007-1323</identifier><identifier>EISSN: 1365-2168</identifier><identifier>DOI: 10.1046/j.1365-2168.1999.01164.x</identifier><identifier>PMID: 10417565</identifier><identifier>CODEN: BJSUAM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adenosine - therapeutic use ; Animals ; Biological and medical sciences ; Blood Pressure ; Cardiovascular Agents - therapeutic use ; Chemokine CXCL2 ; Glutathione - metabolism ; Ischemic Preconditioning - methods ; Male ; Medical sciences ; Monokines - metabolism ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - injuries ; Nitric Oxide - metabolism ; Peroxidase - metabolism ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - prevention & control ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tumor Necrosis Factor-alpha - metabolism ; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels</subject><ispartof>British journal of surgery, 1999-07, Vol.86 (7), p.916-922</ispartof><rights>1999 British Journal of Surgery Society Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4983-7e822f6329752c35e02ce8de1499226c25b71dbc66c9cdb72388223c09a9a05b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2168.1999.01164.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2168.1999.01164.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1894804$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10417565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Papanastasiou, S.</creatorcontrib><creatorcontrib>Estdale, S. E.</creatorcontrib><creatorcontrib>Homer-Vanniasinkam, S.</creatorcontrib><creatorcontrib>Mathie, R. T.</creatorcontrib><title>Protective effect of preconditioning and adenosine pretreatment in experimental skeletal muscle reperfusion injury</title><title>British journal of surgery</title><addtitle>Br J Surg</addtitle><description>Background:
Prolonged ischaemia followed by reperfusion (I/R) of skeletal muscle results in significant tissue injury. Ischaemic preconditioning (IPC), achieved by repeated brief periods of I/R before prolonged ischaemia or adenosine pretreatment, can prevent I/R injury in cardiac muscle. The aim of this study was to ascertain in a rodent model if damage to skeletal muscle due to global hindlimb tourniquet‐induced I/R could be similarly attenuated.
Methods:
Anaesthetized rats were randomized (n = 6–10 per group) to five groups: sham‐operated controls; I/R (4 h of ischaemia, 2 h of reperfusion); IPC (three cycles of 10 min of ischaemia/10 min of reperfusion) alone; IPC immediately preceding I/R; or adenosine 1000 µg/kg immediately before I/R. At the end of reperfusion, biopsies were taken from the left gastrocnemius muscle for measurement of myeloperoxidase (MPO) and reduced glutathione (GSH). Before ischaemia and at the end of reperfusion, blood samples were taken for measurement of nitric oxide metabolites, tumour necrosis factor (TNF) α and macrophage inflammatory protein (MIP) 2.
Results:
IPC before I/R resulted in lower levels of MPO (P < 0·001) and TNF‐α (P = 0·004), and higher levels of GSH (P < 0·001) and nitric oxide metabolites (P = 0·002) than I/R alone. Adenosine had effects comparable to IPC pretreatment (P < 0·001 for MPO, P = 0·002 for GSH, P = 0·02 for nitric oxide metabolites and P = 0·001 for TNF‐α). There was no difference in the blood pressure or the MIP‐2 concentration among the groups.
Conclusion:
IPC or pretreatment with adenosine ameliorates the I/R injury of skeletal muscle. © 2000 British Journal of Surgery Society Ltd</description><subject>Adenosine - therapeutic use</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Pressure</subject><subject>Cardiovascular Agents - therapeutic use</subject><subject>Chemokine CXCL2</subject><subject>Glutathione - metabolism</subject><subject>Ischemic Preconditioning - methods</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Monokines - metabolism</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - injuries</subject><subject>Nitric Oxide - metabolism</subject><subject>Peroxidase - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels</subject><issn>0007-1323</issn><issn>1365-2168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkc1u1DAURq2Kqh1KXwF5gdgl9U9sxxIbqKC0jKASUJaW49wgTzPO1E5g5u1xmGlh5Wvd8x3J_hDClJSUVPJiVVIuRcGorEuqtS4JpbIqt0do8bR4hhaEEFVQzvgpep7SihDKiWAn6DRLqBJSLFC8jcMIbvS_AEPX5QkPHd5EcENo_eiH4MNPbEOLbQthSD7AvB0j2HENYcQ-YNhuIPr5Znuc7qGHeVhPyfWAI-RlN6VsyuxqirsX6LizfYLzw3mGvn94_-3yY7H8cnV9-XZZuErXvFBQM9ZJzrQSzHEBhDmoW6CV1oxJx0SjaNs4KZ12baMYr3OAO6KttkQ0_Ay93ns3cXiYII1m7ZODvrcBhikZOXs4kxl8eQCnZg2t2eTH2Lgzj7-UgVcHwCZn-y7a4Hz6x9W6qkmVsTd77LfvYfefZjZJszJzN2buxsylmb-lma15d_OVKp7jxT7u0wjbp7iN90YqroT58fnKfFou6Y2q7swt_wN4GJte</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Papanastasiou, S.</creator><creator>Estdale, S. E.</creator><creator>Homer-Vanniasinkam, S.</creator><creator>Mathie, R. T.</creator><general>Blackwell Science Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>Protective effect of preconditioning and adenosine pretreatment in experimental skeletal muscle reperfusion injury</title><author>Papanastasiou, S. ; Estdale, S. E. ; Homer-Vanniasinkam, S. ; Mathie, R. T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4983-7e822f6329752c35e02ce8de1499226c25b71dbc66c9cdb72388223c09a9a05b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine - therapeutic use</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Pressure</topic><topic>Cardiovascular Agents - therapeutic use</topic><topic>Chemokine CXCL2</topic><topic>Glutathione - metabolism</topic><topic>Ischemic Preconditioning - methods</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Monokines - metabolism</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - injuries</topic><topic>Nitric Oxide - metabolism</topic><topic>Peroxidase - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papanastasiou, S.</creatorcontrib><creatorcontrib>Estdale, S. E.</creatorcontrib><creatorcontrib>Homer-Vanniasinkam, S.</creatorcontrib><creatorcontrib>Mathie, R. T.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papanastasiou, S.</au><au>Estdale, S. E.</au><au>Homer-Vanniasinkam, S.</au><au>Mathie, R. T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of preconditioning and adenosine pretreatment in experimental skeletal muscle reperfusion injury</atitle><jtitle>British journal of surgery</jtitle><addtitle>Br J Surg</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>86</volume><issue>7</issue><spage>916</spage><epage>922</epage><pages>916-922</pages><issn>0007-1323</issn><eissn>1365-2168</eissn><coden>BJSUAM</coden><abstract>Background:
Prolonged ischaemia followed by reperfusion (I/R) of skeletal muscle results in significant tissue injury. Ischaemic preconditioning (IPC), achieved by repeated brief periods of I/R before prolonged ischaemia or adenosine pretreatment, can prevent I/R injury in cardiac muscle. The aim of this study was to ascertain in a rodent model if damage to skeletal muscle due to global hindlimb tourniquet‐induced I/R could be similarly attenuated.
Methods:
Anaesthetized rats were randomized (n = 6–10 per group) to five groups: sham‐operated controls; I/R (4 h of ischaemia, 2 h of reperfusion); IPC (three cycles of 10 min of ischaemia/10 min of reperfusion) alone; IPC immediately preceding I/R; or adenosine 1000 µg/kg immediately before I/R. At the end of reperfusion, biopsies were taken from the left gastrocnemius muscle for measurement of myeloperoxidase (MPO) and reduced glutathione (GSH). Before ischaemia and at the end of reperfusion, blood samples were taken for measurement of nitric oxide metabolites, tumour necrosis factor (TNF) α and macrophage inflammatory protein (MIP) 2.
Results:
IPC before I/R resulted in lower levels of MPO (P < 0·001) and TNF‐α (P = 0·004), and higher levels of GSH (P < 0·001) and nitric oxide metabolites (P = 0·002) than I/R alone. Adenosine had effects comparable to IPC pretreatment (P < 0·001 for MPO, P = 0·002 for GSH, P = 0·02 for nitric oxide metabolites and P = 0·001 for TNF‐α). There was no difference in the blood pressure or the MIP‐2 concentration among the groups.
Conclusion:
IPC or pretreatment with adenosine ameliorates the I/R injury of skeletal muscle. © 2000 British Journal of Surgery Society Ltd</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10417565</pmid><doi>10.1046/j.1365-2168.1999.01164.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adenosine - therapeutic use Animals Biological and medical sciences Blood Pressure Cardiovascular Agents - therapeutic use Chemokine CXCL2 Glutathione - metabolism Ischemic Preconditioning - methods Male Medical sciences Monokines - metabolism Muscle, Skeletal - blood supply Muscle, Skeletal - injuries Nitric Oxide - metabolism Peroxidase - metabolism Rats Rats, Sprague-Dawley Reperfusion Injury - prevention & control Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tumor Necrosis Factor-alpha - metabolism Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels |
| title | Protective effect of preconditioning and adenosine pretreatment in experimental skeletal muscle reperfusion injury |
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