Calmodulin, gametes and fertilisation
The role of calmodulin in fertilisation events was examined in a zona-free mouse system by using a selective calmodulin inhibitor, calmidazolium (1 μM). The effects of this antagonist were studied either on the ooplasmic calcium oscillations induced by fertilisation by using the Ca2+ indicator, fluo...
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| Veröffentlicht in: | Zygote (Cambridge) 1999-05, Vol.7 (2), p.95-104 |
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| creator | Courtot, Anne-Marie Pesty, Arlette Lefèvre, Brigitte |
| description | The role of calmodulin in fertilisation
events was examined in a zona-free mouse system by using a selective calmodulin inhibitor,
calmidazolium (1 μM). The effects of this antagonist were studied either on the ooplasmic calcium
oscillations induced by fertilisation by using the Ca2+ indicator, fluo-3/AM, or on
pronucleus formation 4 h later by using the nucleic acid stain, Syto-15. When the calmidazolium
treatment was applied to one or the other gamete before insemination, the fertilisation process was
affected only when spermatozoa were treated: most of the oocytes were partially fertilised as
demonstrated by the profile of Ca2+ oscillations and the presence of polar bodies with no
typical male and female pronuclei. When the treatment was applied during insemination, more than half
the oocytes were unfertilised and only a few were partially fertilised. These results demonstrate
that: (1) the calmodulin-dependent events taking place in spermatozoa before insemination appear
essential at least for regular Ca2+ oscillations and for pronucleus formation; (2) the
inhibition of calmodulin by calmidazolium applied to metaphase II oocytes before insemination has no
major impact on their fertilising ability; and (3) at the time of gamete fusion calmodulin, either
from the oocyte or from the spermatozoon, is essential for fertilisation to occur. |
| doi_str_mv | 10.1017/S0967199499000441 |
| format | Article |
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events was examined in a zona-free mouse system by using a selective calmodulin inhibitor,
calmidazolium (1 μM). The effects of this antagonist were studied either on the ooplasmic calcium
oscillations induced by fertilisation by using the Ca2+ indicator, fluo-3/AM, or on
pronucleus formation 4 h later by using the nucleic acid stain, Syto-15. When the calmidazolium
treatment was applied to one or the other gamete before insemination, the fertilisation process was
affected only when spermatozoa were treated: most of the oocytes were partially fertilised as
demonstrated by the profile of Ca2+ oscillations and the presence of polar bodies with no
typical male and female pronuclei. When the treatment was applied during insemination, more than half
the oocytes were unfertilised and only a few were partially fertilised. These results demonstrate
that: (1) the calmodulin-dependent events taking place in spermatozoa before insemination appear
essential at least for regular Ca2+ oscillations and for pronucleus formation; (2) the
inhibition of calmodulin by calmidazolium applied to metaphase II oocytes before insemination has no
major impact on their fertilising ability; and (3) at the time of gamete fusion calmodulin, either
from the oocyte or from the spermatozoon, is essential for fertilisation to occur.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199499000441</identifier><identifier>PMID: 10418102</identifier><language>eng</language><publisher>England: Cambridge University Press</publisher><subject>Acrosome Reaction - drug effects ; Animals ; Calcium - metabolism ; Calcium Signaling ; Calmidazolium ; Calmodulin - antagonists & inhibitors ; Calmodulin - physiology ; Cell Nucleus - physiology ; Confocal imaging ; Enzyme Inhibitors - pharmacology ; Female ; Fertilisation ; Fertilization in Vitro ; Imidazoles - pharmacology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Microscopy, Confocal ; Mouse ; Oocytes - physiology ; Sperm-Ovum Interactions ; Spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology</subject><ispartof>Zygote (Cambridge), 1999-05, Vol.7 (2), p.95-104</ispartof><rights>1999 Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-39a6317e97f66879bae809ed394b519768dfa679fd6e980de540e4ad67a16f053</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0967199499000441/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,777,781,27905,27906,55609</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10418102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Courtot, Anne-Marie</creatorcontrib><creatorcontrib>Pesty, Arlette</creatorcontrib><creatorcontrib>Lefèvre, Brigitte</creatorcontrib><title>Calmodulin, gametes and fertilisation</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>The role of calmodulin in fertilisation
events was examined in a zona-free mouse system by using a selective calmodulin inhibitor,
calmidazolium (1 μM). The effects of this antagonist were studied either on the ooplasmic calcium
oscillations induced by fertilisation by using the Ca2+ indicator, fluo-3/AM, or on
pronucleus formation 4 h later by using the nucleic acid stain, Syto-15. When the calmidazolium
treatment was applied to one or the other gamete before insemination, the fertilisation process was
affected only when spermatozoa were treated: most of the oocytes were partially fertilised as
demonstrated by the profile of Ca2+ oscillations and the presence of polar bodies with no
typical male and female pronuclei. When the treatment was applied during insemination, more than half
the oocytes were unfertilised and only a few were partially fertilised. These results demonstrate
that: (1) the calmodulin-dependent events taking place in spermatozoa before insemination appear
essential at least for regular Ca2+ oscillations and for pronucleus formation; (2) the
inhibition of calmodulin by calmidazolium applied to metaphase II oocytes before insemination has no
major impact on their fertilising ability; and (3) at the time of gamete fusion calmodulin, either
from the oocyte or from the spermatozoon, is essential for fertilisation to occur.</description><subject>Acrosome Reaction - drug effects</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling</subject><subject>Calmidazolium</subject><subject>Calmodulin - antagonists & inhibitors</subject><subject>Calmodulin - physiology</subject><subject>Cell Nucleus - physiology</subject><subject>Confocal imaging</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Female</subject><subject>Fertilisation</subject><subject>Fertilization in Vitro</subject><subject>Imidazoles - pharmacology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>Microscopy, Confocal</subject><subject>Mouse</subject><subject>Oocytes - physiology</subject><subject>Sperm-Ovum Interactions</subject><subject>Spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEURYMotlZ_gBvpRleO5jmZZN5SqlZtQUSly5A2b0rqfNRkBvTfO2WKCIKrt7jnXh6HsWPgF8BBXb5wlAoQBSLnXAjYYX0QEqNUxXyX9TdxtMl77CCEVcsohWKf9YALSIFf9dnpyORFZZvclefDpSmopjA0pR1m5GuXu2BqV5WHbC8zeaCj7R2wt7vb19F9NH0aP4yup9EiVlhHMRoZgyJUmZSpwrmhlCPZGMU8AVQytZmRCjMrCVNuKRGchLFSGZAZT-IBO-t21776aCjUunBhQXluSqqaoCUiyARkC0IHLnwVgqdMr70rjP_SwPXGjf7jpu2cbMebeUH2V6OT0QJRB7hQ0-dPbvy7lipWiZbjZ_2Ik8nNDGZ62vLx9glTzL2zS9KrqvFla-ifN74BYbt7hg</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Courtot, Anne-Marie</creator><creator>Pesty, Arlette</creator><creator>Lefèvre, Brigitte</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Calmodulin, gametes and fertilisation</title><author>Courtot, Anne-Marie ; Pesty, Arlette ; Lefèvre, Brigitte</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-39a6317e97f66879bae809ed394b519768dfa679fd6e980de540e4ad67a16f053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acrosome Reaction - drug effects</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling</topic><topic>Calmidazolium</topic><topic>Calmodulin - antagonists & inhibitors</topic><topic>Calmodulin - physiology</topic><topic>Cell Nucleus - physiology</topic><topic>Confocal imaging</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Female</topic><topic>Fertilisation</topic><topic>Fertilization in Vitro</topic><topic>Imidazoles - pharmacology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>Microscopy, Confocal</topic><topic>Mouse</topic><topic>Oocytes - physiology</topic><topic>Sperm-Ovum Interactions</topic><topic>Spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Courtot, Anne-Marie</creatorcontrib><creatorcontrib>Pesty, Arlette</creatorcontrib><creatorcontrib>Lefèvre, Brigitte</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Courtot, Anne-Marie</au><au>Pesty, Arlette</au><au>Lefèvre, Brigitte</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calmodulin, gametes and fertilisation</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>7</volume><issue>2</issue><spage>95</spage><epage>104</epage><pages>95-104</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>The role of calmodulin in fertilisation
events was examined in a zona-free mouse system by using a selective calmodulin inhibitor,
calmidazolium (1 μM). The effects of this antagonist were studied either on the ooplasmic calcium
oscillations induced by fertilisation by using the Ca2+ indicator, fluo-3/AM, or on
pronucleus formation 4 h later by using the nucleic acid stain, Syto-15. When the calmidazolium
treatment was applied to one or the other gamete before insemination, the fertilisation process was
affected only when spermatozoa were treated: most of the oocytes were partially fertilised as
demonstrated by the profile of Ca2+ oscillations and the presence of polar bodies with no
typical male and female pronuclei. When the treatment was applied during insemination, more than half
the oocytes were unfertilised and only a few were partially fertilised. These results demonstrate
that: (1) the calmodulin-dependent events taking place in spermatozoa before insemination appear
essential at least for regular Ca2+ oscillations and for pronucleus formation; (2) the
inhibition of calmodulin by calmidazolium applied to metaphase II oocytes before insemination has no
major impact on their fertilising ability; and (3) at the time of gamete fusion calmodulin, either
from the oocyte or from the spermatozoon, is essential for fertilisation to occur.</abstract><cop>England</cop><pub>Cambridge University Press</pub><pmid>10418102</pmid><doi>10.1017/S0967199499000441</doi><tpages>10</tpages></addata></record> |
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| ispartof | Zygote (Cambridge), 1999-05, Vol.7 (2), p.95-104 |
| issn | 0967-1994 1469-8730 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69916516 |
| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Acrosome Reaction - drug effects Animals Calcium - metabolism Calcium Signaling Calmidazolium Calmodulin - antagonists & inhibitors Calmodulin - physiology Cell Nucleus - physiology Confocal imaging Enzyme Inhibitors - pharmacology Female Fertilisation Fertilization in Vitro Imidazoles - pharmacology Male Mice Mice, Inbred C57BL Mice, Inbred CBA Microscopy, Confocal Mouse Oocytes - physiology Sperm-Ovum Interactions Spermatozoa Spermatozoa - drug effects Spermatozoa - physiology |
| title | Calmodulin, gametes and fertilisation |
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