Hemosiderin Deposits Confounds Tracking of Iron-Oxide-Labeled Stem Cells: An Experimental Study
Abstract Background The aim of the present research was to study the possible interference of hemosiderin deposits with the histological detection of dextran-coated, iron-labeled, mesenchymal stem cells after intracoronary administration in a porcine model of myocardial infarction. Materials and met...
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| Veröffentlicht in: | Transplantation proceedings 2008-12, Vol.40 (10), p.3619-3622 |
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| creator | Rigol, M Solanes, N Roqué, M Farré, J Batlle, M Roura, S Bellera, N Prat-Vidal, C Sionis, A Ramírez, J Sitges, M Sanz, G Bayés-Genís, A Heras, M |
| description | Abstract Background The aim of the present research was to study the possible interference of hemosiderin deposits with the histological detection of dextran-coated, iron-labeled, mesenchymal stem cells after intracoronary administration in a porcine model of myocardial infarction. Materials and methods A myocardial infarction was induced in six animals that received intracoronary iron-labeled autologous mesenchymal stem cells (group 1; n = 2) or placebo (group 2; n = 4). Six control animals without myocardial infarction underwent direct intramyocardial injections of iron-labeled autologous mesenchymal stem cells (group 3; n = 2) or placebo (group 4; n = 4). Histological sections from explanted hearts were stained with Prussian blue to identify dextran-coated, iron-labeled, mesenchymal stem cells. Results After Prussian blue staining, granular blue labeling in the tissue was observed in both groups of animals with infarcts. Similar granular blue labeling was detected in hearts from control animals without infarction that had received iron-labeled mesenchymal stem cells. However, hearts from control animals without infarction that received placebo did not have any granular blue labeling in the tissue. Conclusions Hemosiderin from infarction hemorrhage interferes with detection of dextran-coated iron-labeled mesenchymal stem cells after intracoronary administration, suggesting that this marker is not useful to detect mesenchymal stem cells in a porcine model of myocardial infarction. |
| doi_str_mv | 10.1016/j.transproceed.2008.05.084 |
| format | Article |
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Materials and methods A myocardial infarction was induced in six animals that received intracoronary iron-labeled autologous mesenchymal stem cells (group 1; n = 2) or placebo (group 2; n = 4). Six control animals without myocardial infarction underwent direct intramyocardial injections of iron-labeled autologous mesenchymal stem cells (group 3; n = 2) or placebo (group 4; n = 4). Histological sections from explanted hearts were stained with Prussian blue to identify dextran-coated, iron-labeled, mesenchymal stem cells. Results After Prussian blue staining, granular blue labeling in the tissue was observed in both groups of animals with infarcts. Similar granular blue labeling was detected in hearts from control animals without infarction that had received iron-labeled mesenchymal stem cells. However, hearts from control animals without infarction that received placebo did not have any granular blue labeling in the tissue. Conclusions Hemosiderin from infarction hemorrhage interferes with detection of dextran-coated iron-labeled mesenchymal stem cells after intracoronary administration, suggesting that this marker is not useful to detect mesenchymal stem cells in a porcine model of myocardial infarction.</description><identifier>ISSN: 0041-1345</identifier><identifier>EISSN: 1873-2623</identifier><identifier>DOI: 10.1016/j.transproceed.2008.05.084</identifier><identifier>PMID: 19100453</identifier><identifier>CODEN: TRPPA8</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell Separation - methods ; Disease Models, Animal ; Ferric Compounds - analysis ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hemosiderin - analysis ; Medical sciences ; Mesenchymal Stromal Cells - cytology ; Myocardial Infarction - pathology ; Myocardial Infarction - surgery ; Stem Cell Transplantation - methods ; Surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Swine ; Tissue, organ and graft immunology ; Transplantation, Autologous</subject><ispartof>Transplantation proceedings, 2008-12, Vol.40 (10), p.3619-3622</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-35a33531844fd32fea28ea8f67db87da75de75d7eb58fd7144aa4e6087560c683</citedby><cites>FETCH-LOGICAL-c463t-35a33531844fd32fea28ea8f67db87da75de75d7eb58fd7144aa4e6087560c683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.transproceed.2008.05.084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20993942$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19100453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rigol, M</creatorcontrib><creatorcontrib>Solanes, N</creatorcontrib><creatorcontrib>Roqué, M</creatorcontrib><creatorcontrib>Farré, J</creatorcontrib><creatorcontrib>Batlle, M</creatorcontrib><creatorcontrib>Roura, S</creatorcontrib><creatorcontrib>Bellera, N</creatorcontrib><creatorcontrib>Prat-Vidal, C</creatorcontrib><creatorcontrib>Sionis, A</creatorcontrib><creatorcontrib>Ramírez, J</creatorcontrib><creatorcontrib>Sitges, M</creatorcontrib><creatorcontrib>Sanz, G</creatorcontrib><creatorcontrib>Bayés-Genís, A</creatorcontrib><creatorcontrib>Heras, M</creatorcontrib><title>Hemosiderin Deposits Confounds Tracking of Iron-Oxide-Labeled Stem Cells: An Experimental Study</title><title>Transplantation proceedings</title><addtitle>Transplant Proc</addtitle><description>Abstract Background The aim of the present research was to study the possible interference of hemosiderin deposits with the histological detection of dextran-coated, iron-labeled, mesenchymal stem cells after intracoronary administration in a porcine model of myocardial infarction. Materials and methods A myocardial infarction was induced in six animals that received intracoronary iron-labeled autologous mesenchymal stem cells (group 1; n = 2) or placebo (group 2; n = 4). Six control animals without myocardial infarction underwent direct intramyocardial injections of iron-labeled autologous mesenchymal stem cells (group 3; n = 2) or placebo (group 4; n = 4). Histological sections from explanted hearts were stained with Prussian blue to identify dextran-coated, iron-labeled, mesenchymal stem cells. Results After Prussian blue staining, granular blue labeling in the tissue was observed in both groups of animals with infarcts. Similar granular blue labeling was detected in hearts from control animals without infarction that had received iron-labeled mesenchymal stem cells. However, hearts from control animals without infarction that received placebo did not have any granular blue labeling in the tissue. Conclusions Hemosiderin from infarction hemorrhage interferes with detection of dextran-coated iron-labeled mesenchymal stem cells after intracoronary administration, suggesting that this marker is not useful to detect mesenchymal stem cells in a porcine model of myocardial infarction.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Separation - methods</subject><subject>Disease Models, Animal</subject><subject>Ferric Compounds - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hemosiderin - analysis</subject><subject>Medical sciences</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Myocardial Infarction - pathology</subject><subject>Myocardial Infarction - surgery</subject><subject>Stem Cell Transplantation - methods</subject><subject>Surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Swine</subject><subject>Tissue, organ and graft immunology</subject><subject>Transplantation, Autologous</subject><issn>0041-1345</issn><issn>1873-2623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktrGzEQgEVpady0f6Eshfa2W2n1WG0OheCkTcCQQ9KzkKXZImctudJuiP99x9iE0lMPQhLzzYOPIeQTow2jTH3dNFO2sexycgC-aSnVDZUN1eIVWTDd8bpVLX9NFpQKVjMu5Bl5V8qG4r8V_C05Yz3Dt-QLYm5gm0rwkEOsrmCH76lUyxSHNEdfqods3WOIv6o0VLc5xfruGeF6Zdcwgq_uJ9hWSxjHclFdxur6eYeFthAnO2Js9vv35M1gxwIfTvc5-fn9-mF5U6_uftwuL1e1E4pPNZeWc8mZFmLwvB3AthqsHlTn17rztpMe8HSwlnrwHRPCWgGK6k4q6pTm5-TLsS5a-T1Dmcw2FIeD2QhpLkb1PVOSSQQvjqDLqZQMg9nhxDbvDaPmoNdszN96zUGvodKgXkz-eOoyr7cYe0k9-UTg8wmwxdlxwEIulBeupX3Pe9Eid3XkAJ08BcimuADRgQ8Z3GR8Cv83z7d_yrgxxICdH2EPZZPmHNG6Yaa0hpr7w0Ic9oFqyihXjP8B_z20vg</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Rigol, M</creator><creator>Solanes, N</creator><creator>Roqué, M</creator><creator>Farré, J</creator><creator>Batlle, M</creator><creator>Roura, S</creator><creator>Bellera, N</creator><creator>Prat-Vidal, C</creator><creator>Sionis, A</creator><creator>Ramírez, J</creator><creator>Sitges, M</creator><creator>Sanz, G</creator><creator>Bayés-Genís, A</creator><creator>Heras, M</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Hemosiderin Deposits Confounds Tracking of Iron-Oxide-Labeled Stem Cells: An Experimental Study</title><author>Rigol, M ; Solanes, N ; Roqué, M ; Farré, J ; Batlle, M ; Roura, S ; Bellera, N ; Prat-Vidal, C ; Sionis, A ; Ramírez, J ; Sitges, M ; Sanz, G ; Bayés-Genís, A ; Heras, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-35a33531844fd32fea28ea8f67db87da75de75d7eb58fd7144aa4e6087560c683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Separation - methods</topic><topic>Disease Models, Animal</topic><topic>Ferric Compounds - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hemosiderin - analysis</topic><topic>Medical sciences</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Myocardial Infarction - pathology</topic><topic>Myocardial Infarction - surgery</topic><topic>Stem Cell Transplantation - methods</topic><topic>Surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Swine</topic><topic>Tissue, organ and graft immunology</topic><topic>Transplantation, Autologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rigol, M</creatorcontrib><creatorcontrib>Solanes, N</creatorcontrib><creatorcontrib>Roqué, M</creatorcontrib><creatorcontrib>Farré, J</creatorcontrib><creatorcontrib>Batlle, M</creatorcontrib><creatorcontrib>Roura, S</creatorcontrib><creatorcontrib>Bellera, N</creatorcontrib><creatorcontrib>Prat-Vidal, C</creatorcontrib><creatorcontrib>Sionis, A</creatorcontrib><creatorcontrib>Ramírez, J</creatorcontrib><creatorcontrib>Sitges, M</creatorcontrib><creatorcontrib>Sanz, G</creatorcontrib><creatorcontrib>Bayés-Genís, A</creatorcontrib><creatorcontrib>Heras, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation proceedings</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rigol, M</au><au>Solanes, N</au><au>Roqué, M</au><au>Farré, J</au><au>Batlle, M</au><au>Roura, S</au><au>Bellera, N</au><au>Prat-Vidal, C</au><au>Sionis, A</au><au>Ramírez, J</au><au>Sitges, M</au><au>Sanz, G</au><au>Bayés-Genís, A</au><au>Heras, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hemosiderin Deposits Confounds Tracking of Iron-Oxide-Labeled Stem Cells: An Experimental Study</atitle><jtitle>Transplantation proceedings</jtitle><addtitle>Transplant Proc</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>40</volume><issue>10</issue><spage>3619</spage><epage>3622</epage><pages>3619-3622</pages><issn>0041-1345</issn><eissn>1873-2623</eissn><coden>TRPPA8</coden><abstract>Abstract Background The aim of the present research was to study the possible interference of hemosiderin deposits with the histological detection of dextran-coated, iron-labeled, mesenchymal stem cells after intracoronary administration in a porcine model of myocardial infarction. Materials and methods A myocardial infarction was induced in six animals that received intracoronary iron-labeled autologous mesenchymal stem cells (group 1; n = 2) or placebo (group 2; n = 4). Six control animals without myocardial infarction underwent direct intramyocardial injections of iron-labeled autologous mesenchymal stem cells (group 3; n = 2) or placebo (group 4; n = 4). Histological sections from explanted hearts were stained with Prussian blue to identify dextran-coated, iron-labeled, mesenchymal stem cells. Results After Prussian blue staining, granular blue labeling in the tissue was observed in both groups of animals with infarcts. Similar granular blue labeling was detected in hearts from control animals without infarction that had received iron-labeled mesenchymal stem cells. However, hearts from control animals without infarction that received placebo did not have any granular blue labeling in the tissue. Conclusions Hemosiderin from infarction hemorrhage interferes with detection of dextran-coated iron-labeled mesenchymal stem cells after intracoronary administration, suggesting that this marker is not useful to detect mesenchymal stem cells in a porcine model of myocardial infarction.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>19100453</pmid><doi>10.1016/j.transproceed.2008.05.084</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Separation - methods Disease Models, Animal Ferric Compounds - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology Hemosiderin - analysis Medical sciences Mesenchymal Stromal Cells - cytology Myocardial Infarction - pathology Myocardial Infarction - surgery Stem Cell Transplantation - methods Surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Swine Tissue, organ and graft immunology Transplantation, Autologous |
| title | Hemosiderin Deposits Confounds Tracking of Iron-Oxide-Labeled Stem Cells: An Experimental Study |
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