Identification and expression of two baculovirus gp37 genes
CG Phanis, DP Miller, SC Cassar, M Tristem, SM Thiem and DR O'Reilly Department of Biology, Imperial College, Imperial College Road, London SW7 2AZ, UK The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and c...
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| Veröffentlicht in: | Journal of general virology 1999-07, Vol.80 (7), p.1823-1831 |
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| creator | Phanis, CG Miller, DP Cassar, SC Tristem, M Thiem, SM O'Reilly, DR |
| description | CG Phanis, DP Miller, SC Cassar, M Tristem, SM Thiem and DR O'Reilly
Department of Biology, Imperial College, Imperial College Road, London SW7 2AZ, UK
The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid
nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and
characterized. Both genes were similar to other baculovirus gp37 genes and
to entomopoxvirus fusolin genes. Phylogenetic analysis showed that
baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct
and well-separated clades. There was no evidence of recent gene transfer
between the two groups. The gp37 genes also showed a distant similarity to
bacterial cellulose- and chitin-binding protein genes, but the significance
of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from
consensus baculovirus late transcription start sites. MbMNPV gp37 was
additionally transcribed from a putative early transcription start site.
Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37
is N-glycosylated. Confocal immunofluorescence microscopy revealed that the
protein is located exclusively in the cytoplasm, probably in the
endoplasmic reticulum. |
| doi_str_mv | 10.1099/0022-1317-80-7-1823 |
| format | Article |
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Department of Biology, Imperial College, Imperial College Road, London SW7 2AZ, UK
The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid
nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and
characterized. Both genes were similar to other baculovirus gp37 genes and
to entomopoxvirus fusolin genes. Phylogenetic analysis showed that
baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct
and well-separated clades. There was no evidence of recent gene transfer
between the two groups. The gp37 genes also showed a distant similarity to
bacterial cellulose- and chitin-binding protein genes, but the significance
of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from
consensus baculovirus late transcription start sites. MbMNPV gp37 was
additionally transcribed from a putative early transcription start site.
Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37
is N-glycosylated. Confocal immunofluorescence microscopy revealed that the
protein is located exclusively in the cytoplasm, probably in the
endoplasmic reticulum.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-80-7-1823</identifier><identifier>PMID: 10423152</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Baculoviridae - genetics ; Baculovirus ; Base Sequence ; Entomopoxvirus ; Gene Expression Regulation, Viral ; Gene Transfer Techniques ; Genes, Viral ; Lymantria dispar ; Mamestra brassicae ; Molecular Sequence Data ; Noctuidae ; Nuclear polyhedrosis virus ; Sequence Alignment ; Viral Envelope Proteins - genetics</subject><ispartof>Journal of general virology, 1999-07, Vol.80 (7), p.1823-1831</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-fb7fb0e0e2037513746f19a7fd4a688c884eecf7a912a3f2f453d56928fc0e923</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3747,3748,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10423152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phanis, CG</creatorcontrib><creatorcontrib>Miller, DP</creatorcontrib><creatorcontrib>Cassar, SC</creatorcontrib><creatorcontrib>Tristem, M</creatorcontrib><creatorcontrib>Thiem, SM</creatorcontrib><creatorcontrib>O'Reilly, DR</creatorcontrib><title>Identification and expression of two baculovirus gp37 genes</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>CG Phanis, DP Miller, SC Cassar, M Tristem, SM Thiem and DR O'Reilly
Department of Biology, Imperial College, Imperial College Road, London SW7 2AZ, UK
The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid
nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and
characterized. Both genes were similar to other baculovirus gp37 genes and
to entomopoxvirus fusolin genes. Phylogenetic analysis showed that
baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct
and well-separated clades. There was no evidence of recent gene transfer
between the two groups. The gp37 genes also showed a distant similarity to
bacterial cellulose- and chitin-binding protein genes, but the significance
of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from
consensus baculovirus late transcription start sites. MbMNPV gp37 was
additionally transcribed from a putative early transcription start site.
Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37
is N-glycosylated. Confocal immunofluorescence microscopy revealed that the
protein is located exclusively in the cytoplasm, probably in the
endoplasmic reticulum.</description><subject>Amino Acid Sequence</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Base Sequence</subject><subject>Entomopoxvirus</subject><subject>Gene Expression Regulation, Viral</subject><subject>Gene Transfer Techniques</subject><subject>Genes, Viral</subject><subject>Lymantria dispar</subject><subject>Mamestra brassicae</subject><subject>Molecular Sequence Data</subject><subject>Noctuidae</subject><subject>Nuclear polyhedrosis virus</subject><subject>Sequence Alignment</subject><subject>Viral Envelope Proteins - genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rr6CwTpSbxE89UmwZMsfiwseNFzSNvJbqRfNq2r_96WLurN0zDMM-8wD0LnlFxTovUNIYxhyqnEimCJqWL8AM2pSGLMhvkhmv8QM3QSwhshVIhYHqMZJYJxGrM5ul3lUHXe-cx2vq4iW-URfDYthDC2tYu6XR2lNuuL-sO3fYg2DZfRBioIp-jI2SLA2b4u0OvD_cvyCa-fH1fLuzXOhEw67FLpUgIEGOEyplyKxFFtpcuFTZTKlBIAmZNWU2a5Y07EPI8TzZTLCGjGF-hyym3a-r2H0JnShwyKwlZQ98EkWg-PKf0vSCVnRCTJAPIJzNo6hBacaVpf2vbLUGJGuWZUZ0Z1RhEjzSh32LrYx_dpCfmfncnmAFxNwNZvtjvfghk8lX44kvraDPp-s74BuXqB5A</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Phanis, CG</creator><creator>Miller, DP</creator><creator>Cassar, SC</creator><creator>Tristem, M</creator><creator>Thiem, SM</creator><creator>O'Reilly, DR</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>Identification and expression of two baculovirus gp37 genes</title><author>Phanis, CG ; Miller, DP ; Cassar, SC ; Tristem, M ; Thiem, SM ; O'Reilly, DR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-fb7fb0e0e2037513746f19a7fd4a688c884eecf7a912a3f2f453d56928fc0e923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Base Sequence</topic><topic>Entomopoxvirus</topic><topic>Gene Expression Regulation, Viral</topic><topic>Gene Transfer Techniques</topic><topic>Genes, Viral</topic><topic>Lymantria dispar</topic><topic>Mamestra brassicae</topic><topic>Molecular Sequence Data</topic><topic>Noctuidae</topic><topic>Nuclear polyhedrosis virus</topic><topic>Sequence Alignment</topic><topic>Viral Envelope Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phanis, CG</creatorcontrib><creatorcontrib>Miller, DP</creatorcontrib><creatorcontrib>Cassar, SC</creatorcontrib><creatorcontrib>Tristem, M</creatorcontrib><creatorcontrib>Thiem, SM</creatorcontrib><creatorcontrib>O'Reilly, DR</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phanis, CG</au><au>Miller, DP</au><au>Cassar, SC</au><au>Tristem, M</au><au>Thiem, SM</au><au>O'Reilly, DR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and expression of two baculovirus gp37 genes</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>80</volume><issue>7</issue><spage>1823</spage><epage>1831</epage><pages>1823-1831</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>CG Phanis, DP Miller, SC Cassar, M Tristem, SM Thiem and DR O'Reilly
Department of Biology, Imperial College, Imperial College Road, London SW7 2AZ, UK
The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid
nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and
characterized. Both genes were similar to other baculovirus gp37 genes and
to entomopoxvirus fusolin genes. Phylogenetic analysis showed that
baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct
and well-separated clades. There was no evidence of recent gene transfer
between the two groups. The gp37 genes also showed a distant similarity to
bacterial cellulose- and chitin-binding protein genes, but the significance
of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from
consensus baculovirus late transcription start sites. MbMNPV gp37 was
additionally transcribed from a putative early transcription start site.
Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37
is N-glycosylated. Confocal immunofluorescence microscopy revealed that the
protein is located exclusively in the cytoplasm, probably in the
endoplasmic reticulum.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>10423152</pmid><doi>10.1099/0022-1317-80-7-1823</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Baculoviridae - genetics Baculovirus Base Sequence Entomopoxvirus Gene Expression Regulation, Viral Gene Transfer Techniques Genes, Viral Lymantria dispar Mamestra brassicae Molecular Sequence Data Noctuidae Nuclear polyhedrosis virus Sequence Alignment Viral Envelope Proteins - genetics |
| title | Identification and expression of two baculovirus gp37 genes |
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