Purification and characterization of protease produced by Staphylococcus aureus isolated from a diseased chicken

A protease produced by Staphylococcus aureus, isolated from a chicken suffering from dermatitis, was purified by successive precipitation with ammonium sulfate, ion-exchange chromatography on Q-Sepharose FF, Sp-Sepharose FF and Mono-Q columns. By Mono-Q column chromatography, two proteases (protease...

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Veröffentlicht in:	Veterinary microbiology 1999-06, Vol.67 (3), p.195-202
Hauptverfasser:	Takeuchi, Shotaro, Kinoshita, Takafumi, Kaidoh, Toshio, Hashizume, Naoko
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Ammonium Sulfate - chemistry Animals Bacteriology Biological and medical sciences Blotting, Western - veterinary Caseins - chemistry Chemical Precipitation Chicken Chickens Chromatography, Ion Exchange - veterinary Cysteine - chemistry Dermatitis - microbiology Dermatitis - veterinary Electrophoresis, Polyacrylamide Gel - veterinary Endopeptidases - chemistry Endopeptidases - isolation & purification Fundamental and applied biological sciences. Psychology Immune Sera - biosynthesis Isoelectric Focusing - veterinary Metabolism. Enzymes Microbiology Molecular Sequence Data Molecular Weight Poultry Diseases - microbiology Protease Purification Rabbits Sequence Analysis Staphylococcus aureus Staphylococcus aureus - chemistry Staphylococcus aureus - enzymology Thioglycolates - chemistry
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