Piroxicam quantitation in human plasma by high-performance liquid chromatography with on- and off-line solid-phase extraction
A comparative study of two analytical methodologies for piroxicam quantitation in plasma by off-line and on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contained C 8 for both extraction methods. The analytes piroxicam and tenox...
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| Veröffentlicht in: | Journal of Chromatography A 1999-06, Vol.846 (1), p.199-205 |
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| container_title | Journal of Chromatography A |
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| creator | Yritia, M Parra, P Fernández, J.M Barbanoj, J.M |
| description | A comparative study of two analytical methodologies for piroxicam quantitation in plasma by off-line and on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contained C
8 for both extraction methods. The analytes piroxicam and tenoxican (internal standard) were separated on a C
18 column with a mobile phase consisting of acetonitrile:20 m
M phosphate buffer pH 3.1 (50:50, v/v) followed by UV detection at 360 nm. The validation of the methods demonstrated good recoveries (over 90%), sensitivity (limits of quantification of 0.05 μg/ml with on-line SPE and 0.1 μg/ml with off-line SPE, based on a 100 μl and 200 μl sample volume, respectively), accuracy and precision (better than 9.5%). Both methodologies have been used for bioequivalence studies. |
| doi_str_mv | 10.1016/S0021-9673(99)00515-4 |
| format | Article |
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8 for both extraction methods. The analytes piroxicam and tenoxican (internal standard) were separated on a C
18 column with a mobile phase consisting of acetonitrile:20 m
M phosphate buffer pH 3.1 (50:50, v/v) followed by UV detection at 360 nm. The validation of the methods demonstrated good recoveries (over 90%), sensitivity (limits of quantification of 0.05 μg/ml with on-line SPE and 0.1 μg/ml with off-line SPE, based on a 100 μl and 200 μl sample volume, respectively), accuracy and precision (better than 9.5%). Both methodologies have been used for bioequivalence studies.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(99)00515-4</identifier><identifier>PMID: 10420612</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Anti-Inflammatory Agents, Non-Steroidal - blood ; Anti-Inflammatory Agents, Non-Steroidal - pharmacokinetics ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; General pharmacology ; Humans ; Medical sciences ; Pharmacology. Drug treatments ; Piroxicam ; Piroxicam - blood ; Piroxicam - pharmacokinetics ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrophotometry, Ultraviolet</subject><ispartof>Journal of Chromatography A, 1999-06, Vol.846 (1), p.199-205</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-a9bcf68c52fea2d86480fbd4f8e4682d490fa479a8514acde941bb2e5a444e193</citedby><cites>FETCH-LOGICAL-c390t-a9bcf68c52fea2d86480fbd4f8e4682d490fa479a8514acde941bb2e5a444e193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967399005154$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1879877$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10420612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yritia, M</creatorcontrib><creatorcontrib>Parra, P</creatorcontrib><creatorcontrib>Fernández, J.M</creatorcontrib><creatorcontrib>Barbanoj, J.M</creatorcontrib><title>Piroxicam quantitation in human plasma by high-performance liquid chromatography with on- and off-line solid-phase extraction</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A comparative study of two analytical methodologies for piroxicam quantitation in plasma by off-line and on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contained C
8 for both extraction methods. The analytes piroxicam and tenoxican (internal standard) were separated on a C
18 column with a mobile phase consisting of acetonitrile:20 m
M phosphate buffer pH 3.1 (50:50, v/v) followed by UV detection at 360 nm. The validation of the methods demonstrated good recoveries (over 90%), sensitivity (limits of quantification of 0.05 μg/ml with on-line SPE and 0.1 μg/ml with off-line SPE, based on a 100 μl and 200 μl sample volume, respectively), accuracy and precision (better than 9.5%). Both methodologies have been used for bioequivalence studies.</description><subject>Analysis</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - blood</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Piroxicam</subject><subject>Piroxicam - blood</subject><subject>Piroxicam - pharmacokinetics</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhn0AtaXlJ4B8QAgOBtvrfPiEqoovqVKRgLM1sceNURJnbQe6h_53st0VcOM00szzzoweQp4J_kZwUb_9yrkUTNfN5pXWrzmvRMXUI3L2p31KnuT8g3PR8EaekFPBleS1kGfk_ktI8S5YGOl2gamEAiXEiYaJ9ssIE50HyCPQbkf7cNuzGZOPaR1YpEPYLsFR26c4Qom3CeZ-R3-F0tM4MQqTo9F7NoQJaY5DcGzuISPFu5LA7s9ckMcehoxPj_WcfP_w_tvVJ3Z98_Hz1eU1sxvNCwPdWV-3tpIeQbq2Vi33nVO-RVW30inNPahGQ1sJBdahVqLrJFaglEKhN-fk5WHvnOJ2wVzMGLLFYYAJ45JNrbUQWvIVrA6gTTHnhN7MKYyQdkZws3dtHlybvVSjtXlwbdSae348sHQjun9SB9Er8OIIQLYw-LQaDPkv1za6bZoVe3fAcLXxM2Ay2QZcZbuQ0BbjYvjPJ78BEkWgGw</recordid><startdate>19990618</startdate><enddate>19990618</enddate><creator>Yritia, M</creator><creator>Parra, P</creator><creator>Fernández, J.M</creator><creator>Barbanoj, J.M</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990618</creationdate><title>Piroxicam quantitation in human plasma by high-performance liquid chromatography with on- and off-line solid-phase extraction</title><author>Yritia, M ; Parra, P ; Fernández, J.M ; Barbanoj, J.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-a9bcf68c52fea2d86480fbd4f8e4682d490fa479a8514acde941bb2e5a444e193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Analysis</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - blood</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Piroxicam</topic><topic>Piroxicam - blood</topic><topic>Piroxicam - pharmacokinetics</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yritia, M</creatorcontrib><creatorcontrib>Parra, P</creatorcontrib><creatorcontrib>Fernández, J.M</creatorcontrib><creatorcontrib>Barbanoj, J.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yritia, M</au><au>Parra, P</au><au>Fernández, J.M</au><au>Barbanoj, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Piroxicam quantitation in human plasma by high-performance liquid chromatography with on- and off-line solid-phase extraction</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1999-06-18</date><risdate>1999</risdate><volume>846</volume><issue>1</issue><spage>199</spage><epage>205</epage><pages>199-205</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A comparative study of two analytical methodologies for piroxicam quantitation in plasma by off-line and on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contained C
8 for both extraction methods. The analytes piroxicam and tenoxican (internal standard) were separated on a C
18 column with a mobile phase consisting of acetonitrile:20 m
M phosphate buffer pH 3.1 (50:50, v/v) followed by UV detection at 360 nm. The validation of the methods demonstrated good recoveries (over 90%), sensitivity (limits of quantification of 0.05 μg/ml with on-line SPE and 0.1 μg/ml with off-line SPE, based on a 100 μl and 200 μl sample volume, respectively), accuracy and precision (better than 9.5%). Both methodologies have been used for bioequivalence studies.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10420612</pmid><doi>10.1016/S0021-9673(99)00515-4</doi><tpages>7</tpages></addata></record> |
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| subjects | Analysis Anti-Inflammatory Agents, Non-Steroidal - blood Anti-Inflammatory Agents, Non-Steroidal - pharmacokinetics Biological and medical sciences Chromatography, High Pressure Liquid - methods General pharmacology Humans Medical sciences Pharmacology. Drug treatments Piroxicam Piroxicam - blood Piroxicam - pharmacokinetics Reference Standards Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet |
| title | Piroxicam quantitation in human plasma by high-performance liquid chromatography with on- and off-line solid-phase extraction |
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