Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells
Abstract This investigation examined the effects of zinc status on cell proliferation and the synergic roles of the metallothionein (MT) and zinc transporter (ZnT) in the human colon adenocarcinoma cell line Caco-2. Cells were treated with 0 to 300 μmol/L ZnSO4 or 0 to 10 μmol/L N , N , N ′, N ′-tet...
Gespeichert in:
| Veröffentlicht in: | Nutrition research (New York, N.Y.) N.Y.), 2008-06, Vol.28 (6), p.406-413 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 413 |
|---|---|
| container_issue | 6 |
| container_start_page | 406 |
| container_title | Nutrition research (New York, N.Y.) |
| container_volume | 28 |
| creator | Shen, Hui Qin, Haihong Guo, Junsheng |
| description | Abstract This investigation examined the effects of zinc status on cell proliferation and the synergic roles of the metallothionein (MT) and zinc transporter (ZnT) in the human colon adenocarcinoma cell line Caco-2. Cells were treated with 0 to 300 μmol/L ZnSO4 or 0 to 10 μmol/L N , N , N ′, N ′-tetrakis(2-phridylmethyl) ethylenediamine (TPEN). Cell proliferation was determined by MTT assay and apoptotic cells detected by flow cytometry (Hoechst 33258 dye). mRNA expression of MT1; ZnT1; zrt, irt-like protein 1, 4 (ZIP1, 4); and divalent metal transporter (DMT1) were determined by the reverse transcription polymerase chain reaction or real-time polymerase chain reaction. The results showed that either high or low zinc could inhibit the cell proliferation. The number of apoptotic cells increased with incremental increases in the concentrations of ZnSO4 and TPEN. The mRNA expression of ZnT1 and MT1 responded significantly after 6 and 12 hours with 200 μmol/L zinc treatment, respectively, and increased gradually with zinc levels from 0 to 200 μmol/L. Compared with the unchanged ZIP1 mRNA expression, ZIP4 was closely dependent on TPEN treatment duration and concentration. The DMT1 mRNA expression was upregulated time-dependently but not concentration-dependently in the late TPEN treatment duration. The results suggest that ZIP4 and DMT1 mRNA expressions are susceptible to low extracellular zinc concentration and upregulated to enhance zinc absorption, whereas the ZnT1 and MT1 act as the key regulators under high zinc conditions to enhance the intracellular zinc efflux to maintain zinc homeostasis. We propose that in response to variations in zinc concentration, the cooperated regulative roles of ZnT1, MT1, DMT1, and ZIP4 contribute to zinc homeostasis. |
| doi_str_mv | 10.1016/j.nutres.2008.02.011 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69902818</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0271531708000390</els_id><sourcerecordid>69902818</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-a2d8bc310df4b551b081148094cf4a7c11f933b4cc12f6a7707f7335906cb79c3</originalsourceid><addsrcrecordid>eNqFkl2L1DAUhoso7rj6D0R7o3cdz0kyTXMjLINfsODFutchTZOZjG0yJqmw_npTOih441USeN7Dy5NTVS8RtgjYvjtt_ZyjSVsC0G2BbAHxUbXBjosGOBWPqw0Qjs2OIr-qnqV0AkCOlD6trlBARxkVm2reh3A2UWUXfB1sPZmsxjHkY3kb52vlh_qX87rOUfl0DjGbmGobYh3NYR5Lzh9W4BgmE1JWyaW6BI_zpHy5ZJMKo8Z6r3RoSK3NOKbn1ROrxmReXM7r6v7jh2_7z83t109f9je3jWatyI0iQ9drijBY1u922EOHyDoQTFumuEa0gtKeaY3Etopz4JZTuhPQ6p4LTa-rt-vccww_5tJETi4tDZQ3YU6yFQJIh10B2QrqGFKKxspzdJOKDxJBLrrlSa665aJbApFFd4m9usyf-8kMf0MXvwV4cwFU0mq0RaJ26Q9HgDHKO1K41ytnVZDqEAtzf0cAKUBp2BJWiPcrYYqvn85EmbQzXpvBRaOzHIL7X9d_B-jReVdafTcPJp3CHMs3JYkylYC8W5Zn2R3oAIAKoL8Bc9O_vg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69902818</pqid></control><display><type>article</type><title>Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Shen, Hui ; Qin, Haihong ; Guo, Junsheng</creator><creatorcontrib>Shen, Hui ; Qin, Haihong ; Guo, Junsheng</creatorcontrib><description>Abstract This investigation examined the effects of zinc status on cell proliferation and the synergic roles of the metallothionein (MT) and zinc transporter (ZnT) in the human colon adenocarcinoma cell line Caco-2. Cells were treated with 0 to 300 μmol/L ZnSO4 or 0 to 10 μmol/L N , N , N ′, N ′-tetrakis(2-phridylmethyl) ethylenediamine (TPEN). Cell proliferation was determined by MTT assay and apoptotic cells detected by flow cytometry (Hoechst 33258 dye). mRNA expression of MT1; ZnT1; zrt, irt-like protein 1, 4 (ZIP1, 4); and divalent metal transporter (DMT1) were determined by the reverse transcription polymerase chain reaction or real-time polymerase chain reaction. The results showed that either high or low zinc could inhibit the cell proliferation. The number of apoptotic cells increased with incremental increases in the concentrations of ZnSO4 and TPEN. The mRNA expression of ZnT1 and MT1 responded significantly after 6 and 12 hours with 200 μmol/L zinc treatment, respectively, and increased gradually with zinc levels from 0 to 200 μmol/L. Compared with the unchanged ZIP1 mRNA expression, ZIP4 was closely dependent on TPEN treatment duration and concentration. The DMT1 mRNA expression was upregulated time-dependently but not concentration-dependently in the late TPEN treatment duration. The results suggest that ZIP4 and DMT1 mRNA expressions are susceptible to low extracellular zinc concentration and upregulated to enhance zinc absorption, whereas the ZnT1 and MT1 act as the key regulators under high zinc conditions to enhance the intracellular zinc efflux to maintain zinc homeostasis. We propose that in response to variations in zinc concentration, the cooperated regulative roles of ZnT1, MT1, DMT1, and ZIP4 contribute to zinc homeostasis.</description><identifier>ISSN: 0271-5317</identifier><identifier>EISSN: 1879-0739</identifier><identifier>DOI: 10.1016/j.nutres.2008.02.011</identifier><identifier>PMID: 19083439</identifier><identifier>CODEN: NTRSDC</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2 H-tetrazolium bromide ; acrodermatitis enteropathica ; adenocarcinoma ; analysis of variance ; ANOVA ; apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Caco-2 cells ; Caco-2 Cells - drug effects ; cation diffusion facilitator ; Cation Transport Proteins - biosynthesis ; Cation Transport Proteins - genetics ; CDF ; cell culture ; cell proliferation ; Cell Proliferation - drug effects ; colorectal neoplasms ; Divalent metal transporter ; DMT1 ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; GAPDH ; Gastroenterology and Hepatology ; Gene Expression Regulation ; glyceraldehyde-3-phosphate dehydrogenase ; homeostasis ; Homeostasis - drug effects ; human cell lines ; Humans ; irt-like protein ; messenger RNA ; metal transporter ; Metallothionein ; Metallothionein - biosynthesis ; Metallothionein - genetics ; MTT ; N, N, N′, N′-tetrakis(2-phridylmethyl) ethylenediamine ; nutrition-genotype interaction ; nutritional status ; PCR ; polymerase chain reaction ; propidium iodide ; reverse transcriptase polymerase chain reaction ; RNA, Messenger - biosynthesis ; TPEN ; transporters ; Up-Regulation ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Zinc ; Zinc - metabolism ; Zinc - pharmacology ; Zinc transporter ; ZIP ; ZnT ; Zrt ; zrt, irt-like protein</subject><ispartof>Nutrition research (New York, N.Y.), 2008-06, Vol.28 (6), p.406-413</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-a2d8bc310df4b551b081148094cf4a7c11f933b4cc12f6a7707f7335906cb79c3</citedby><cites>FETCH-LOGICAL-c469t-a2d8bc310df4b551b081148094cf4a7c11f933b4cc12f6a7707f7335906cb79c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nutres.2008.02.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20443782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19083439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Hui</creatorcontrib><creatorcontrib>Qin, Haihong</creatorcontrib><creatorcontrib>Guo, Junsheng</creatorcontrib><title>Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells</title><title>Nutrition research (New York, N.Y.)</title><addtitle>Nutr Res</addtitle><description>Abstract This investigation examined the effects of zinc status on cell proliferation and the synergic roles of the metallothionein (MT) and zinc transporter (ZnT) in the human colon adenocarcinoma cell line Caco-2. Cells were treated with 0 to 300 μmol/L ZnSO4 or 0 to 10 μmol/L N , N , N ′, N ′-tetrakis(2-phridylmethyl) ethylenediamine (TPEN). Cell proliferation was determined by MTT assay and apoptotic cells detected by flow cytometry (Hoechst 33258 dye). mRNA expression of MT1; ZnT1; zrt, irt-like protein 1, 4 (ZIP1, 4); and divalent metal transporter (DMT1) were determined by the reverse transcription polymerase chain reaction or real-time polymerase chain reaction. The results showed that either high or low zinc could inhibit the cell proliferation. The number of apoptotic cells increased with incremental increases in the concentrations of ZnSO4 and TPEN. The mRNA expression of ZnT1 and MT1 responded significantly after 6 and 12 hours with 200 μmol/L zinc treatment, respectively, and increased gradually with zinc levels from 0 to 200 μmol/L. Compared with the unchanged ZIP1 mRNA expression, ZIP4 was closely dependent on TPEN treatment duration and concentration. The DMT1 mRNA expression was upregulated time-dependently but not concentration-dependently in the late TPEN treatment duration. The results suggest that ZIP4 and DMT1 mRNA expressions are susceptible to low extracellular zinc concentration and upregulated to enhance zinc absorption, whereas the ZnT1 and MT1 act as the key regulators under high zinc conditions to enhance the intracellular zinc efflux to maintain zinc homeostasis. We propose that in response to variations in zinc concentration, the cooperated regulative roles of ZnT1, MT1, DMT1, and ZIP4 contribute to zinc homeostasis.</description><subject>3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2 H-tetrazolium bromide</subject><subject>acrodermatitis enteropathica</subject><subject>adenocarcinoma</subject><subject>analysis of variance</subject><subject>ANOVA</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Caco-2 cells</subject><subject>Caco-2 Cells - drug effects</subject><subject>cation diffusion facilitator</subject><subject>Cation Transport Proteins - biosynthesis</subject><subject>Cation Transport Proteins - genetics</subject><subject>CDF</subject><subject>cell culture</subject><subject>cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>colorectal neoplasms</subject><subject>Divalent metal transporter</subject><subject>DMT1</subject><subject>Feeding. Feeding behavior</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAPDH</subject><subject>Gastroenterology and Hepatology</subject><subject>Gene Expression Regulation</subject><subject>glyceraldehyde-3-phosphate dehydrogenase</subject><subject>homeostasis</subject><subject>Homeostasis - drug effects</subject><subject>human cell lines</subject><subject>Humans</subject><subject>irt-like protein</subject><subject>messenger RNA</subject><subject>metal transporter</subject><subject>Metallothionein</subject><subject>Metallothionein - biosynthesis</subject><subject>Metallothionein - genetics</subject><subject>MTT</subject><subject>N, N, N′, N′-tetrakis(2-phridylmethyl) ethylenediamine</subject><subject>nutrition-genotype interaction</subject><subject>nutritional status</subject><subject>PCR</subject><subject>polymerase chain reaction</subject><subject>propidium iodide</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>TPEN</subject><subject>transporters</subject><subject>Up-Regulation</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Zinc</subject><subject>Zinc - metabolism</subject><subject>Zinc - pharmacology</subject><subject>Zinc transporter</subject><subject>ZIP</subject><subject>ZnT</subject><subject>Zrt</subject><subject>zrt, irt-like protein</subject><issn>0271-5317</issn><issn>1879-0739</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl2L1DAUhoso7rj6D0R7o3cdz0kyTXMjLINfsODFutchTZOZjG0yJqmw_npTOih441USeN7Dy5NTVS8RtgjYvjtt_ZyjSVsC0G2BbAHxUbXBjosGOBWPqw0Qjs2OIr-qnqV0AkCOlD6trlBARxkVm2reh3A2UWUXfB1sPZmsxjHkY3kb52vlh_qX87rOUfl0DjGbmGobYh3NYR5Lzh9W4BgmE1JWyaW6BI_zpHy5ZJMKo8Z6r3RoSK3NOKbn1ROrxmReXM7r6v7jh2_7z83t109f9je3jWatyI0iQ9drijBY1u922EOHyDoQTFumuEa0gtKeaY3Etopz4JZTuhPQ6p4LTa-rt-vccww_5tJETi4tDZQ3YU6yFQJIh10B2QrqGFKKxspzdJOKDxJBLrrlSa665aJbApFFd4m9usyf-8kMf0MXvwV4cwFU0mq0RaJ26Q9HgDHKO1K41ytnVZDqEAtzf0cAKUBp2BJWiPcrYYqvn85EmbQzXpvBRaOzHIL7X9d_B-jReVdafTcPJp3CHMs3JYkylYC8W5Zn2R3oAIAKoL8Bc9O_vg</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Shen, Hui</creator><creator>Qin, Haihong</creator><creator>Guo, Junsheng</creator><general>Elsevier Inc</general><general>Tarrytown, N.Y.: Elsevier Science Inc</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080601</creationdate><title>Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells</title><author>Shen, Hui ; Qin, Haihong ; Guo, Junsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-a2d8bc310df4b551b081148094cf4a7c11f933b4cc12f6a7707f7335906cb79c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2 H-tetrazolium bromide</topic><topic>acrodermatitis enteropathica</topic><topic>adenocarcinoma</topic><topic>analysis of variance</topic><topic>ANOVA</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Caco-2 cells</topic><topic>Caco-2 Cells - drug effects</topic><topic>cation diffusion facilitator</topic><topic>Cation Transport Proteins - biosynthesis</topic><topic>Cation Transport Proteins - genetics</topic><topic>CDF</topic><topic>cell culture</topic><topic>cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>colorectal neoplasms</topic><topic>Divalent metal transporter</topic><topic>DMT1</topic><topic>Feeding. Feeding behavior</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAPDH</topic><topic>Gastroenterology and Hepatology</topic><topic>Gene Expression Regulation</topic><topic>glyceraldehyde-3-phosphate dehydrogenase</topic><topic>homeostasis</topic><topic>Homeostasis - drug effects</topic><topic>human cell lines</topic><topic>Humans</topic><topic>irt-like protein</topic><topic>messenger RNA</topic><topic>metal transporter</topic><topic>Metallothionein</topic><topic>Metallothionein - biosynthesis</topic><topic>Metallothionein - genetics</topic><topic>MTT</topic><topic>N, N, N′, N′-tetrakis(2-phridylmethyl) ethylenediamine</topic><topic>nutrition-genotype interaction</topic><topic>nutritional status</topic><topic>PCR</topic><topic>polymerase chain reaction</topic><topic>propidium iodide</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>TPEN</topic><topic>transporters</topic><topic>Up-Regulation</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><topic>Zinc - pharmacology</topic><topic>Zinc transporter</topic><topic>ZIP</topic><topic>ZnT</topic><topic>Zrt</topic><topic>zrt, irt-like protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Hui</creatorcontrib><creatorcontrib>Qin, Haihong</creatorcontrib><creatorcontrib>Guo, Junsheng</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nutrition research (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Hui</au><au>Qin, Haihong</au><au>Guo, Junsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells</atitle><jtitle>Nutrition research (New York, N.Y.)</jtitle><addtitle>Nutr Res</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>28</volume><issue>6</issue><spage>406</spage><epage>413</epage><pages>406-413</pages><issn>0271-5317</issn><eissn>1879-0739</eissn><coden>NTRSDC</coden><abstract>Abstract This investigation examined the effects of zinc status on cell proliferation and the synergic roles of the metallothionein (MT) and zinc transporter (ZnT) in the human colon adenocarcinoma cell line Caco-2. Cells were treated with 0 to 300 μmol/L ZnSO4 or 0 to 10 μmol/L N , N , N ′, N ′-tetrakis(2-phridylmethyl) ethylenediamine (TPEN). Cell proliferation was determined by MTT assay and apoptotic cells detected by flow cytometry (Hoechst 33258 dye). mRNA expression of MT1; ZnT1; zrt, irt-like protein 1, 4 (ZIP1, 4); and divalent metal transporter (DMT1) were determined by the reverse transcription polymerase chain reaction or real-time polymerase chain reaction. The results showed that either high or low zinc could inhibit the cell proliferation. The number of apoptotic cells increased with incremental increases in the concentrations of ZnSO4 and TPEN. The mRNA expression of ZnT1 and MT1 responded significantly after 6 and 12 hours with 200 μmol/L zinc treatment, respectively, and increased gradually with zinc levels from 0 to 200 μmol/L. Compared with the unchanged ZIP1 mRNA expression, ZIP4 was closely dependent on TPEN treatment duration and concentration. The DMT1 mRNA expression was upregulated time-dependently but not concentration-dependently in the late TPEN treatment duration. The results suggest that ZIP4 and DMT1 mRNA expressions are susceptible to low extracellular zinc concentration and upregulated to enhance zinc absorption, whereas the ZnT1 and MT1 act as the key regulators under high zinc conditions to enhance the intracellular zinc efflux to maintain zinc homeostasis. We propose that in response to variations in zinc concentration, the cooperated regulative roles of ZnT1, MT1, DMT1, and ZIP4 contribute to zinc homeostasis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19083439</pmid><doi>10.1016/j.nutres.2008.02.011</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0271-5317 |
| ispartof | Nutrition research (New York, N.Y.), 2008-06, Vol.28 (6), p.406-413 |
| issn | 0271-5317 1879-0739 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69902818 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2 H-tetrazolium bromide acrodermatitis enteropathica adenocarcinoma analysis of variance ANOVA apoptosis Apoptosis - drug effects Biological and medical sciences Caco-2 cells Caco-2 Cells - drug effects cation diffusion facilitator Cation Transport Proteins - biosynthesis Cation Transport Proteins - genetics CDF cell culture cell proliferation Cell Proliferation - drug effects colorectal neoplasms Divalent metal transporter DMT1 Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology GAPDH Gastroenterology and Hepatology Gene Expression Regulation glyceraldehyde-3-phosphate dehydrogenase homeostasis Homeostasis - drug effects human cell lines Humans irt-like protein messenger RNA metal transporter Metallothionein Metallothionein - biosynthesis Metallothionein - genetics MTT N, N, N′, N′-tetrakis(2-phridylmethyl) ethylenediamine nutrition-genotype interaction nutritional status PCR polymerase chain reaction propidium iodide reverse transcriptase polymerase chain reaction RNA, Messenger - biosynthesis TPEN transporters Up-Regulation Vertebrates: anatomy and physiology, studies on body, several organs or systems Zinc Zinc - metabolism Zinc - pharmacology Zinc transporter ZIP ZnT Zrt zrt, irt-like protein |
| title | Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T02%3A07%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cooperation%20of%20metallothionein%20and%20zinc%20transporters%20for%20regulating%20zinc%20homeostasis%20in%20human%20intestinal%20Caco-2%20cells&rft.jtitle=Nutrition%20research%20(New%20York,%20N.Y.)&rft.au=Shen,%20Hui&rft.date=2008-06-01&rft.volume=28&rft.issue=6&rft.spage=406&rft.epage=413&rft.pages=406-413&rft.issn=0271-5317&rft.eissn=1879-0739&rft.coden=NTRSDC&rft_id=info:doi/10.1016/j.nutres.2008.02.011&rft_dat=%3Cproquest_cross%3E69902818%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69902818&rft_id=info:pmid/19083439&rft_els_id=S0271531708000390&rfr_iscdi=true |