Changes of myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia: experiment with pigs
To investigate the changes myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia and to evaluate the relationship between the gene expression of glycolytic metabolism related enzymes and myocardial viability. Fourteen Chinese experimental pigs underwent pl...
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| Veröffentlicht in: | Zhong hua yi xue za zhi 2008-08, Vol.88 (31), p.2209-2213 |
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| container_title | Zhong hua yi xue za zhi |
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| creator | Gong, Jing Wang, Hong-yue Pu, Jie-lin Zheng, Ying-li Shen, Rui Yang, Min-fu He, Zuo-xiang |
| description | To investigate the changes myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia and to evaluate the relationship between the gene expression of glycolytic metabolism related enzymes and myocardial viability.
Fourteen Chinese experimental pigs underwent placement of arterial ring into the left anterior descending coronary artery so as to establish models of myocardial ischemia and infarction. (18)F-2-deoxy-2-fluoro-D-glucose single photon emission computed tomography was conducted to observe the viability of the myocardium. One week later the pigs were killed with their hearts taken out. Specimens of ischemic zone, infarction zone, and non-ischemic zone were obtained. RT-PCR was used to detect the mRNA expression of glucose transporter (GLUT) 1, GLUT4, medium-chain acyl-CoA dehydrogenase (MCAD), and heart-fatty acid binding protein (H-FABP). Immunohistochemistry was used to examine the protein expression of Glut1 and Glut4. Periodic Acid Schiff-hematoxylin staining |
| format | Article |
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Fourteen Chinese experimental pigs underwent placement of arterial ring into the left anterior descending coronary artery so as to establish models of myocardial ischemia and infarction. (18)F-2-deoxy-2-fluoro-D-glucose single photon emission computed tomography was conducted to observe the viability of the myocardium. One week later the pigs were killed with their hearts taken out. Specimens of ischemic zone, infarction zone, and non-ischemic zone were obtained. RT-PCR was used to detect the mRNA expression of glucose transporter (GLUT) 1, GLUT4, medium-chain acyl-CoA dehydrogenase (MCAD), and heart-fatty acid binding protein (H-FABP). Immunohistochemistry was used to examine the protein expression of Glut1 and Glut4. Periodic Acid Schiff-hematoxylin staining</description><identifier>ISSN: 0376-2491</identifier><identifier>PMID: 19080674</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Chronic Disease ; Fatty Acids - metabolism ; Female ; Glucose - metabolism ; Glucose Transporter Type 1 - genetics ; Glucose Transporter Type 1 - metabolism ; Glucose Transporter Type 4 - genetics ; Glucose Transporter Type 4 - metabolism ; Glycolysis ; Immunohistochemistry ; Lipid Metabolism ; Male ; Myocardial Ischemia - enzymology ; Myocardial Ischemia - genetics ; Myocardial Ischemia - metabolism ; Myocardium - enzymology ; Myocardium - metabolism ; Myocardium - pathology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Swine</subject><ispartof>Zhong hua yi xue za zhi, 2008-08, Vol.88 (31), p.2209-2213</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19080674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Jing</creatorcontrib><creatorcontrib>Wang, Hong-yue</creatorcontrib><creatorcontrib>Pu, Jie-lin</creatorcontrib><creatorcontrib>Zheng, Ying-li</creatorcontrib><creatorcontrib>Shen, Rui</creatorcontrib><creatorcontrib>Yang, Min-fu</creatorcontrib><creatorcontrib>He, Zuo-xiang</creatorcontrib><title>Changes of myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia: experiment with pigs</title><title>Zhong hua yi xue za zhi</title><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><description>To investigate the changes myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia and to evaluate the relationship between the gene expression of glycolytic metabolism related enzymes and myocardial viability.
Fourteen Chinese experimental pigs underwent placement of arterial ring into the left anterior descending coronary artery so as to establish models of myocardial ischemia and infarction. (18)F-2-deoxy-2-fluoro-D-glucose single photon emission computed tomography was conducted to observe the viability of the myocardium. One week later the pigs were killed with their hearts taken out. Specimens of ischemic zone, infarction zone, and non-ischemic zone were obtained. RT-PCR was used to detect the mRNA expression of glucose transporter (GLUT) 1, GLUT4, medium-chain acyl-CoA dehydrogenase (MCAD), and heart-fatty acid binding protein (H-FABP). Immunohistochemistry was used to examine the protein expression of Glut1 and Glut4. Periodic Acid Schiff-hematoxylin staining</description><subject>Animals</subject><subject>Chronic Disease</subject><subject>Fatty Acids - metabolism</subject><subject>Female</subject><subject>Glucose - metabolism</subject><subject>Glucose Transporter Type 1 - genetics</subject><subject>Glucose Transporter Type 1 - metabolism</subject><subject>Glucose Transporter Type 4 - genetics</subject><subject>Glucose Transporter Type 4 - metabolism</subject><subject>Glycolysis</subject><subject>Immunohistochemistry</subject><subject>Lipid Metabolism</subject><subject>Male</subject><subject>Myocardial Ischemia - enzymology</subject><subject>Myocardial Ischemia - genetics</subject><subject>Myocardial Ischemia - metabolism</subject><subject>Myocardium - enzymology</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - pathology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Swine</subject><issn>0376-2491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLxDAYRbtQnGGcvyBZuSskbSZN3MngCwQ3ui55fJkG8qhNBq1Lf7kFR3B14XK4HO5ZtcZtx-qGCrKqtjk7hWnXigY35KJaEYE5Zh1dV9_7QcYDZJQsCnPScjJOegTxaw5LO4GXBQwqCR38rJOfs8tIRoOsLGVGUjuDAhSpknc5IBeRHqYUnf6_5rIeIDh5g-BzhMkFiAV9uDKg0R3yZXVupc-wPeWmeru_e90_1s8vD0_72-d6JA0tNds1nSCEgOJcYsMNtUwBtowJYNSAtlYRrKzWdAemwVhy3VlDCVeGdoy0m-r6d3ec0vsRcunDIgbeywjpmHsmxPIO5gt4dQKPKoDpx8VYTnP_91r7A9vjbGk</recordid><startdate>20080812</startdate><enddate>20080812</enddate><creator>Gong, Jing</creator><creator>Wang, Hong-yue</creator><creator>Pu, Jie-lin</creator><creator>Zheng, Ying-li</creator><creator>Shen, Rui</creator><creator>Yang, Min-fu</creator><creator>He, Zuo-xiang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20080812</creationdate><title>Changes of myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia: experiment with pigs</title><author>Gong, Jing ; Wang, Hong-yue ; Pu, Jie-lin ; Zheng, Ying-li ; Shen, Rui ; Yang, Min-fu ; He, Zuo-xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-65279111eb88a0d8d4f6be0f669e64decffb10bfcc45ed200a8c7fd418bd47613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Chronic Disease</topic><topic>Fatty Acids - metabolism</topic><topic>Female</topic><topic>Glucose - metabolism</topic><topic>Glucose Transporter Type 1 - genetics</topic><topic>Glucose Transporter Type 1 - metabolism</topic><topic>Glucose Transporter Type 4 - genetics</topic><topic>Glucose Transporter Type 4 - metabolism</topic><topic>Glycolysis</topic><topic>Immunohistochemistry</topic><topic>Lipid Metabolism</topic><topic>Male</topic><topic>Myocardial Ischemia - enzymology</topic><topic>Myocardial Ischemia - genetics</topic><topic>Myocardial Ischemia - metabolism</topic><topic>Myocardium - enzymology</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - pathology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Swine</topic><toplevel>online_resources</toplevel><creatorcontrib>Gong, Jing</creatorcontrib><creatorcontrib>Wang, Hong-yue</creatorcontrib><creatorcontrib>Pu, Jie-lin</creatorcontrib><creatorcontrib>Zheng, Ying-li</creatorcontrib><creatorcontrib>Shen, Rui</creatorcontrib><creatorcontrib>Yang, Min-fu</creatorcontrib><creatorcontrib>He, Zuo-xiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong hua yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Jing</au><au>Wang, Hong-yue</au><au>Pu, Jie-lin</au><au>Zheng, Ying-li</au><au>Shen, Rui</au><au>Yang, Min-fu</au><au>He, Zuo-xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes of myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia: experiment with pigs</atitle><jtitle>Zhong hua yi xue za zhi</jtitle><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><date>2008-08-12</date><risdate>2008</risdate><volume>88</volume><issue>31</issue><spage>2209</spage><epage>2213</epage><pages>2209-2213</pages><issn>0376-2491</issn><abstract>To investigate the changes myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia and to evaluate the relationship between the gene expression of glycolytic metabolism related enzymes and myocardial viability.
Fourteen Chinese experimental pigs underwent placement of arterial ring into the left anterior descending coronary artery so as to establish models of myocardial ischemia and infarction. (18)F-2-deoxy-2-fluoro-D-glucose single photon emission computed tomography was conducted to observe the viability of the myocardium. One week later the pigs were killed with their hearts taken out. Specimens of ischemic zone, infarction zone, and non-ischemic zone were obtained. RT-PCR was used to detect the mRNA expression of glucose transporter (GLUT) 1, GLUT4, medium-chain acyl-CoA dehydrogenase (MCAD), and heart-fatty acid binding protein (H-FABP). Immunohistochemistry was used to examine the protein expression of Glut1 and Glut4. Periodic Acid Schiff-hematoxylin staining</abstract><cop>China</cop><pmid>19080674</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Freely accessible e-journals |
| subjects | Animals Chronic Disease Fatty Acids - metabolism Female Glucose - metabolism Glucose Transporter Type 1 - genetics Glucose Transporter Type 1 - metabolism Glucose Transporter Type 4 - genetics Glucose Transporter Type 4 - metabolism Glycolysis Immunohistochemistry Lipid Metabolism Male Myocardial Ischemia - enzymology Myocardial Ischemia - genetics Myocardial Ischemia - metabolism Myocardium - enzymology Myocardium - metabolism Myocardium - pathology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Swine |
| title | Changes of myocardial enzymes related to glycolysis and fatty acid metabolism in chronic myocardial ischemia: experiment with pigs |
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