Transgenic tomato plants with decreased sucrose synthase are unaltered in starch and sugar accumulation in the fruit
Sucrose is the photoassimilate transported from the leaves to the fruit of tomato yet the fruit accumulates predominantly glucose and fructose. Hydrolysis of sucrose entering the fruit can be accomplished by invertase or sucrose synthase. Early in tomato fruit development there is a transient increa...
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| Veröffentlicht in: | Plant molecular biology 1999-05, Vol.40 (2), p.213-221 |
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| container_title | Plant molecular biology |
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| creator | Chengappa, S Guilleroux, M Phillips, W Shields, R |
| description | Sucrose is the photoassimilate transported from the leaves to the fruit of tomato yet the fruit accumulates predominantly glucose and fructose. Hydrolysis of sucrose entering the fruit can be accomplished by invertase or sucrose synthase. Early in tomato fruit development there is a transient increase in sucrose synthase activity and starch which is correlated with fruit growth and sink strength suggesting a regulatory role for sucrose synthase in sugar import. Using an antisense sucrose synthase cDNA under the control of a fruit-specific promoter we show that sucrose synthase activity can be reduced by up to 99% in young fruit without affecting starch or sugar accumulation. This result calls into question the importance of sucrose synthase in regulating sink strength in tomato fruit. |
| doi_str_mv | 10.1023/A:1006136524725 |
| format | Article |
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Hydrolysis of sucrose entering the fruit can be accomplished by invertase or sucrose synthase. Early in tomato fruit development there is a transient increase in sucrose synthase activity and starch which is correlated with fruit growth and sink strength suggesting a regulatory role for sucrose synthase in sugar import. Using an antisense sucrose synthase cDNA under the control of a fruit-specific promoter we show that sucrose synthase activity can be reduced by up to 99% in young fruit without affecting starch or sugar accumulation. This result calls into question the importance of sucrose synthase in regulating sink strength in tomato fruit.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1006136524725</identifier><identifier>PMID: 10412901</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>antisense DNA ; Cell Wall - metabolism ; cell wall components ; chemical constituents of plants ; Cloning, Molecular ; complementary DNA ; enzyme activity ; Fructose - metabolism ; fruit specific promoters ; Fruits ; gene expression ; genetic regulation ; genetic transformation ; Glucose - metabolism ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; Hexoses - metabolism ; Lycopersicon esculentum ; Lycopersicon esculentum - enzymology ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - metabolism ; Plants, Genetically Modified ; promoter regions ; recombinant DNA ; Recombinant Fusion Proteins - genetics ; reducing sugars ; Solanum lycopersicum var. lycopersicum ; Space life sciences ; Starch ; Starch - metabolism ; Sucrose ; sucrose synthase ; Sugar ; Tomatoes ; transgenic plants</subject><ispartof>Plant molecular biology, 1999-05, Vol.40 (2), p.213-221</ispartof><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-fa0c4a3e2868e6547052fbbc96870b3d70730559fd5f4e3153a27c403a76837f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10412901$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chengappa, S</creatorcontrib><creatorcontrib>Guilleroux, M</creatorcontrib><creatorcontrib>Phillips, W</creatorcontrib><creatorcontrib>Shields, R</creatorcontrib><title>Transgenic tomato plants with decreased sucrose synthase are unaltered in starch and sugar accumulation in the fruit</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Sucrose is the photoassimilate transported from the leaves to the fruit of tomato yet the fruit accumulates predominantly glucose and fructose. Hydrolysis of sucrose entering the fruit can be accomplished by invertase or sucrose synthase. Early in tomato fruit development there is a transient increase in sucrose synthase activity and starch which is correlated with fruit growth and sink strength suggesting a regulatory role for sucrose synthase in sugar import. Using an antisense sucrose synthase cDNA under the control of a fruit-specific promoter we show that sucrose synthase activity can be reduced by up to 99% in young fruit without affecting starch or sugar accumulation. This result calls into question the importance of sucrose synthase in regulating sink strength in tomato fruit.</description><subject>antisense DNA</subject><subject>Cell Wall - metabolism</subject><subject>cell wall components</subject><subject>chemical constituents of plants</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>enzyme activity</subject><subject>Fructose - metabolism</subject><subject>fruit specific promoters</subject><subject>Fruits</subject><subject>gene expression</subject><subject>genetic regulation</subject><subject>genetic transformation</subject><subject>Glucose - metabolism</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucosyltransferases - metabolism</subject><subject>Hexoses - metabolism</subject><subject>Lycopersicon esculentum</subject><subject>Lycopersicon esculentum - enzymology</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Lycopersicon esculentum - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>promoter regions</subject><subject>recombinant DNA</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>reducing sugars</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>Space life sciences</subject><subject>Starch</subject><subject>Starch - metabolism</subject><subject>Sucrose</subject><subject>sucrose synthase</subject><subject>Sugar</subject><subject>Tomatoes</subject><subject>transgenic plants</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0LtPHEEMB-ARSgQHSZ0uGVHQLXjG89hNhxAQJKQUHPXKNzt7t2gfl3ko4r_PnCBNmlSW7E-2_GPsi4BLARKvrr8LACPQaKms1EdsJbTFSoOsP7AVCGMrpYQ8YacxvgAUjOaYnQgozQbEiqV1oDlu_Tw4npaJ0sL3I80p8t9D2vHOu-Ap-o7H7MISPY-vc9qVDqfgeZ5pTD6U8TDzmCi4Haf5gLcUODmXpzxSGpb5ANLO8z7kIX1iH3sao__8Xs_Y893t-uZH9fjz_uHm-rFyiDpVPYFThF7WpvZGKwta9puNa0xtYYOdBYugddN3ulcehUaS1ilAsqZG2-MZu3jbuw_Lr-xjaqchOj-WB_2SY2uauqkl4n-hKIfqEnOB5__AlyWHkkJsrRVKYzlc0Nd3lDeT79p9GCYKr-3f1Av49gZ6WlrahiG2z0-yDEA2ogFl8Q-SsY0i</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Chengappa, S</creator><creator>Guilleroux, M</creator><creator>Phillips, W</creator><creator>Shields, R</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Transgenic tomato plants with decreased sucrose synthase are unaltered in starch and sugar accumulation in the fruit</title><author>Chengappa, S ; Guilleroux, M ; Phillips, W ; Shields, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-fa0c4a3e2868e6547052fbbc96870b3d70730559fd5f4e3153a27c403a76837f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>antisense DNA</topic><topic>Cell Wall - 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Hydrolysis of sucrose entering the fruit can be accomplished by invertase or sucrose synthase. Early in tomato fruit development there is a transient increase in sucrose synthase activity and starch which is correlated with fruit growth and sink strength suggesting a regulatory role for sucrose synthase in sugar import. Using an antisense sucrose synthase cDNA under the control of a fruit-specific promoter we show that sucrose synthase activity can be reduced by up to 99% in young fruit without affecting starch or sugar accumulation. This result calls into question the importance of sucrose synthase in regulating sink strength in tomato fruit.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10412901</pmid><doi>10.1023/A:1006136524725</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant molecular biology, 1999-05, Vol.40 (2), p.213-221 |
| issn | 0167-4412 1573-5028 |
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| subjects | antisense DNA Cell Wall - metabolism cell wall components chemical constituents of plants Cloning, Molecular complementary DNA enzyme activity Fructose - metabolism fruit specific promoters Fruits gene expression genetic regulation genetic transformation Glucose - metabolism Glucosyltransferases - genetics Glucosyltransferases - metabolism Hexoses - metabolism Lycopersicon esculentum Lycopersicon esculentum - enzymology Lycopersicon esculentum - genetics Lycopersicon esculentum - metabolism Plants, Genetically Modified promoter regions recombinant DNA Recombinant Fusion Proteins - genetics reducing sugars Solanum lycopersicum var. lycopersicum Space life sciences Starch Starch - metabolism Sucrose sucrose synthase Sugar Tomatoes transgenic plants |
| title | Transgenic tomato plants with decreased sucrose synthase are unaltered in starch and sugar accumulation in the fruit |
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