Comparison of Protein Synthesis Patterns in Mouse Cumulus Cells and Mural Granulosa Cells: Effects of Follicle-Stimulating Hormone and Insulin on Granulosa Cell Differentiation In Vitro
Successful development of mammalian oocytes requires correct interactions between developing oocytes and associated granulosa cells. Development of oocyte-granulosa cell complexes from preantral follicles in vitro does not produce oocytes competent to develop to blastocysts at the same frequency as...
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| Veröffentlicht in: | Biology of reproduction 1999-08, Vol.61 (2), p.482-492 |
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| creator | LATHAM, K. E BAUTISTA, F. D. M HIRAO, Y O'BRIEN, M. J EPPIG, J. J |
| description | Successful development of mammalian oocytes requires correct interactions between developing oocytes and associated granulosa
cells. Development of oocyte-granulosa cell complexes from preantral follicles in vitro does not produce oocytes competent
to develop to blastocysts at the same frequency as for oocytes that develop in vivo. Addition of either FSH or insulin to
cultures of oocyte-granulosa cell complexes does not improve the frequency of blastocyst development, and the combination
of both insulin and FSH is deleterious. Here, high-resolution 2-dimensional PAGE (2D-PAGE) and computerized gel image analysis
were used to compare patterns of protein synthesis in cumulus cells and mural granulosa cells of small antral follicles, and
then to assess effects of FSH and insulin on the differentiation of oocyte-associated granulosa cells (OAGCs) in vitro. Culture
of OAGCs without FSH or insulin resulted in failure to synthesize many proteins at rates characteristic of cumulus cells.
Either hormone used alone caused many cumulus cell proteins that were decreased in control cultures to be synthesized at nearly
normal cumulus cell rates, and also caused the synthesis of other proteins to be increased or decreased. The two hormones
added together produced the greatest change in protein synthetic pattern, including overexpression or underexpression of many
proteins not affected by either hormone alone. Addition of these hormones to culture media thus appeared insufficient to elicit
a normal cumulus cell phenotype in OAGCs and could lead to complex changes in protein synthesis that may be deleterious to
oocyte development. The high-resolution 2D-PAGE approach described here should be a valuable tool in studies on oocyte and
granulosa cell development in vitro, since phenotype can be evaluated globally through the display of over 1000 newly synthesized
proteins rather than relying upon the expression of just a few genes. |
| doi_str_mv | 10.1095/biolreprod61.2.482 |
| format | Article |
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cells. Development of oocyte-granulosa cell complexes from preantral follicles in vitro does not produce oocytes competent
to develop to blastocysts at the same frequency as for oocytes that develop in vivo. Addition of either FSH or insulin to
cultures of oocyte-granulosa cell complexes does not improve the frequency of blastocyst development, and the combination
of both insulin and FSH is deleterious. Here, high-resolution 2-dimensional PAGE (2D-PAGE) and computerized gel image analysis
were used to compare patterns of protein synthesis in cumulus cells and mural granulosa cells of small antral follicles, and
then to assess effects of FSH and insulin on the differentiation of oocyte-associated granulosa cells (OAGCs) in vitro. Culture
of OAGCs without FSH or insulin resulted in failure to synthesize many proteins at rates characteristic of cumulus cells.
Either hormone used alone caused many cumulus cell proteins that were decreased in control cultures to be synthesized at nearly
normal cumulus cell rates, and also caused the synthesis of other proteins to be increased or decreased. The two hormones
added together produced the greatest change in protein synthetic pattern, including overexpression or underexpression of many
proteins not affected by either hormone alone. Addition of these hormones to culture media thus appeared insufficient to elicit
a normal cumulus cell phenotype in OAGCs and could lead to complex changes in protein synthesis that may be deleterious to
oocyte development. The high-resolution 2D-PAGE approach described here should be a valuable tool in studies on oocyte and
granulosa cell development in vitro, since phenotype can be evaluated globally through the display of over 1000 newly synthesized
proteins rather than relying upon the expression of just a few genes.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod61.2.482</identifier><identifier>PMID: 10411531</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cells, Cultured ; Culture Media ; Electrophoresis, Gel, Two-Dimensional ; Female ; Follicle Stimulating Hormone - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; Hormone metabolism and regulation ; Insulin - pharmacology ; Mammalian female genital system ; Mice ; Oocytes - drug effects ; Oocytes - metabolism ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1999-08, Vol.61 (2), p.482-492</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1911262$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10411531$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LATHAM, K. E</creatorcontrib><creatorcontrib>BAUTISTA, F. D. M</creatorcontrib><creatorcontrib>HIRAO, Y</creatorcontrib><creatorcontrib>O'BRIEN, M. J</creatorcontrib><creatorcontrib>EPPIG, J. J</creatorcontrib><title>Comparison of Protein Synthesis Patterns in Mouse Cumulus Cells and Mural Granulosa Cells: Effects of Follicle-Stimulating Hormone and Insulin on Granulosa Cell Differentiation In Vitro</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Successful development of mammalian oocytes requires correct interactions between developing oocytes and associated granulosa
cells. Development of oocyte-granulosa cell complexes from preantral follicles in vitro does not produce oocytes competent
to develop to blastocysts at the same frequency as for oocytes that develop in vivo. Addition of either FSH or insulin to
cultures of oocyte-granulosa cell complexes does not improve the frequency of blastocyst development, and the combination
of both insulin and FSH is deleterious. Here, high-resolution 2-dimensional PAGE (2D-PAGE) and computerized gel image analysis
were used to compare patterns of protein synthesis in cumulus cells and mural granulosa cells of small antral follicles, and
then to assess effects of FSH and insulin on the differentiation of oocyte-associated granulosa cells (OAGCs) in vitro. Culture
of OAGCs without FSH or insulin resulted in failure to synthesize many proteins at rates characteristic of cumulus cells.
Either hormone used alone caused many cumulus cell proteins that were decreased in control cultures to be synthesized at nearly
normal cumulus cell rates, and also caused the synthesis of other proteins to be increased or decreased. The two hormones
added together produced the greatest change in protein synthetic pattern, including overexpression or underexpression of many
proteins not affected by either hormone alone. Addition of these hormones to culture media thus appeared insufficient to elicit
a normal cumulus cell phenotype in OAGCs and could lead to complex changes in protein synthesis that may be deleterious to
oocyte development. The high-resolution 2D-PAGE approach described here should be a valuable tool in studies on oocyte and
granulosa cell development in vitro, since phenotype can be evaluated globally through the display of over 1000 newly synthesized
proteins rather than relying upon the expression of just a few genes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - metabolism</subject><subject>Hormone metabolism and regulation</subject><subject>Insulin - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Mice</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctu1DAUhi0EotPCC7BAXkB3GXxJnJgdCr2M1IpKBbaRkxx3jBx76otGfTTeDsMMQqws-Xzn8zm_EXpDyZoS2XwYjbcBdsHPgq7Zuu7YM7SiDZNVy0T3HK0IIaLiXPATdBrjD0JozRl_iU4oqSltOF2hn71fdiqY6B32Gt8Fn8A4fP_k0haiifhOpQTBRVxub32OgPu8ZJsj7sHaiJWb8W0OyuKroFy2PqpD5SO-0BqmFH97L721ZrJQ3SdTulUy7gFf-7B4B38UGxezLU-UMf734M-maAK4ZEpXKW8c_m5S8K_QC61shNfH8wx9u7z42l9XN1-uNv2nm2rLhEgVb6nqBCF6pISPtYKWjRIUmWEkup0ZcDLSpqGCEuiI1qwduaQwFl43vO74GTo_eEvQjxliGhYTpzKZclDyGITspJCyLuDbI5jHBeZhF8yiwtPwN-wCvDsCKk7K6rLnZOI_TlLKBCvY-wO2NQ_bvQkwxEVZW6x82O_3gg5sKH_NfwGYh6C4</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>LATHAM, K. E</creator><creator>BAUTISTA, F. D. M</creator><creator>HIRAO, Y</creator><creator>O'BRIEN, M. J</creator><creator>EPPIG, J. J</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19990801</creationdate><title>Comparison of Protein Synthesis Patterns in Mouse Cumulus Cells and Mural Granulosa Cells: Effects of Follicle-Stimulating Hormone and Insulin on Granulosa Cell Differentiation In Vitro</title><author>LATHAM, K. E ; BAUTISTA, F. D. M ; HIRAO, Y ; O'BRIEN, M. J ; EPPIG, J. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-371a8600fb103b4ae72b9ea0deb0f7d2e30b1551610e80ff27b391ebfb1f53483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Granulosa Cells - drug effects</topic><topic>Granulosa Cells - metabolism</topic><topic>Hormone metabolism and regulation</topic><topic>Insulin - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Mice</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LATHAM, K. E</creatorcontrib><creatorcontrib>BAUTISTA, F. D. M</creatorcontrib><creatorcontrib>HIRAO, Y</creatorcontrib><creatorcontrib>O'BRIEN, M. J</creatorcontrib><creatorcontrib>EPPIG, J. 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J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Protein Synthesis Patterns in Mouse Cumulus Cells and Mural Granulosa Cells: Effects of Follicle-Stimulating Hormone and Insulin on Granulosa Cell Differentiation In Vitro</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>61</volume><issue>2</issue><spage>482</spage><epage>492</epage><pages>482-492</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Successful development of mammalian oocytes requires correct interactions between developing oocytes and associated granulosa
cells. Development of oocyte-granulosa cell complexes from preantral follicles in vitro does not produce oocytes competent
to develop to blastocysts at the same frequency as for oocytes that develop in vivo. Addition of either FSH or insulin to
cultures of oocyte-granulosa cell complexes does not improve the frequency of blastocyst development, and the combination
of both insulin and FSH is deleterious. Here, high-resolution 2-dimensional PAGE (2D-PAGE) and computerized gel image analysis
were used to compare patterns of protein synthesis in cumulus cells and mural granulosa cells of small antral follicles, and
then to assess effects of FSH and insulin on the differentiation of oocyte-associated granulosa cells (OAGCs) in vitro. Culture
of OAGCs without FSH or insulin resulted in failure to synthesize many proteins at rates characteristic of cumulus cells.
Either hormone used alone caused many cumulus cell proteins that were decreased in control cultures to be synthesized at nearly
normal cumulus cell rates, and also caused the synthesis of other proteins to be increased or decreased. The two hormones
added together produced the greatest change in protein synthetic pattern, including overexpression or underexpression of many
proteins not affected by either hormone alone. Addition of these hormones to culture media thus appeared insufficient to elicit
a normal cumulus cell phenotype in OAGCs and could lead to complex changes in protein synthesis that may be deleterious to
oocyte development. The high-resolution 2D-PAGE approach described here should be a valuable tool in studies on oocyte and
granulosa cell development in vitro, since phenotype can be evaluated globally through the display of over 1000 newly synthesized
proteins rather than relying upon the expression of just a few genes.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>10411531</pmid><doi>10.1095/biolreprod61.2.482</doi><tpages>11</tpages></addata></record> |
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| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69896994 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Cell Differentiation - drug effects Cells, Cultured Culture Media Electrophoresis, Gel, Two-Dimensional Female Follicle Stimulating Hormone - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Granulosa Cells - drug effects Granulosa Cells - metabolism Hormone metabolism and regulation Insulin - pharmacology Mammalian female genital system Mice Oocytes - drug effects Oocytes - metabolism Vertebrates: reproduction |
| title | Comparison of Protein Synthesis Patterns in Mouse Cumulus Cells and Mural Granulosa Cells: Effects of Follicle-Stimulating Hormone and Insulin on Granulosa Cell Differentiation In Vitro |
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