Distribution of mRNA encoding a nitrobenzylthioinosine-insensitive nucleoside transporter (ENT2) in rat brain
Nucleoside transporters may play a role in regulating levels of extracellular adenosine and adenosine receptor activity. Two members of the equilibrative nucleoside transporter family have recently been cloned. ENT1 is potently inhibited by nitrobenzylthioinosine (NBMPR) ( K i ≈1 nM) and was previou...
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| Veröffentlicht in: | Brain research. Molecular brain research. 1999-07, Vol.70 (2), p.293-297 |
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| container_title | Brain research. Molecular brain research. |
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| creator | Anderson, Christopher M Baldwin, Stephen A Young, James D Cass, Carol E Parkinson, Fiona E |
| description | Nucleoside transporters may play a role in regulating levels of extracellular adenosine and adenosine receptor activity. Two members of the equilibrative nucleoside transporter family have recently been cloned. ENT1 is potently inhibited by nitrobenzylthioinosine (NBMPR) (
K
i
≈1 nM) and was previously found to have a wide distribution in rat and human brain. ENT2 is insensitive to inhibition by NBMPR at low nanomolar concentrations and there is limited information describing its distribution in rat brain. The present study used RT-PCR, northern blot and in situ hybridization and detected rENT2 transcript in several brain regions including hippocampus, cortex, striatum and cerebellum. Our results indicate a wide cellular and regional distribution for ENT2 in rat brain, similar to ENT1, indicating that control of adenosine levels in brain is achieved by multiple transport processes. |
| doi_str_mv | 10.1016/S0169-328X(99)00164-3 |
| format | Article |
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K
i
≈1 nM) and was previously found to have a wide distribution in rat and human brain. ENT2 is insensitive to inhibition by NBMPR at low nanomolar concentrations and there is limited information describing its distribution in rat brain. The present study used RT-PCR, northern blot and in situ hybridization and detected rENT2 transcript in several brain regions including hippocampus, cortex, striatum and cerebellum. Our results indicate a wide cellular and regional distribution for ENT2 in rat brain, similar to ENT1, indicating that control of adenosine levels in brain is achieved by multiple transport processes.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/S0169-328X(99)00164-3</identifier><identifier>PMID: 10407178</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenosine ; Adenosine transport ; Anatomy ; Animals ; Biological and medical sciences ; Brain - metabolism ; Carrier Proteins - genetics ; Central nervous system ; Cerebrovascular Circulation - physiology ; Choroid Plexus - metabolism ; Ei nucleoside transporter ; ENT2 ; Fundamental and applied biological sciences. Psychology ; Hippocampus - metabolism ; Humans ; In Situ Hybridization ; Membrane Proteins - genetics ; Nitrobenzylthioinosine ; Nucleoside Transport Proteins ; Organ Specificity ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; RT-PCR ; Transcription, Genetic ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research. Molecular brain research., 1999-07, Vol.70 (2), p.293-297</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-f8e8e4f55af067195963e5da6a9f9a73518a25c40a2ef3c25ba363e9be4e9913</citedby><cites>FETCH-LOGICAL-c473t-f8e8e4f55af067195963e5da6a9f9a73518a25c40a2ef3c25ba363e9be4e9913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1916498$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10407178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, Christopher M</creatorcontrib><creatorcontrib>Baldwin, Stephen A</creatorcontrib><creatorcontrib>Young, James D</creatorcontrib><creatorcontrib>Cass, Carol E</creatorcontrib><creatorcontrib>Parkinson, Fiona E</creatorcontrib><title>Distribution of mRNA encoding a nitrobenzylthioinosine-insensitive nucleoside transporter (ENT2) in rat brain</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>Nucleoside transporters may play a role in regulating levels of extracellular adenosine and adenosine receptor activity. Two members of the equilibrative nucleoside transporter family have recently been cloned. ENT1 is potently inhibited by nitrobenzylthioinosine (NBMPR) (
K
i
≈1 nM) and was previously found to have a wide distribution in rat and human brain. ENT2 is insensitive to inhibition by NBMPR at low nanomolar concentrations and there is limited information describing its distribution in rat brain. The present study used RT-PCR, northern blot and in situ hybridization and detected rENT2 transcript in several brain regions including hippocampus, cortex, striatum and cerebellum. Our results indicate a wide cellular and regional distribution for ENT2 in rat brain, similar to ENT1, indicating that control of adenosine levels in brain is achieved by multiple transport processes.</description><subject>Adenosine</subject><subject>Adenosine transport</subject><subject>Anatomy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Carrier Proteins - genetics</subject><subject>Central nervous system</subject><subject>Cerebrovascular Circulation - physiology</subject><subject>Choroid Plexus - metabolism</subject><subject>Ei nucleoside transporter</subject><subject>ENT2</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus - metabolism</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Membrane Proteins - genetics</subject><subject>Nitrobenzylthioinosine</subject><subject>Nucleoside Transport Proteins</subject><subject>Organ Specificity</subject><subject>Rats</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>RT-PCR</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9vFCEUB3BiNHat_gkaDsa0h1EY5gfvZJraqklTE92DN8Iwb_SZWViBaVL_-rLdjXrrBQL5vAd5X8ZeSvFWCtm9-1YWqFStv58AnIpyair1iK2k7uuqg0Y-Zqu_5Ig9S-mXKEpL-ZQdSdGIXvZ6xTYfKOVIw5IpeB4mvvl6fcbRuzCS_8Et95RjGND_uZ3zTwrkQyKPFfmEPlGmG-R-cTOW6xF5jtanbYgZIz-5uF7Xp5w8jzbzIVryz9mTyc4JXxz2Y7a-vFiff6quvnz8fH52VbmmV7maNGpspra1k-h6CS10CtvRdhYmsL1qpbZ16xpha5yUq9vBqiJgwAYBpDpmb_ZttzH8XjBls6HkcJ6tx7Ak04GGVgt4EMq-1tArUWC7hy6GlCJOZhtpY-OtkcLs8jD3eZjdsA2Auc_DqFL36vDAMmxw_K9qH0ABrw_AJmfnqczPUfrnoPSBHXu_Z1imdkMYTXJUUsKRIrpsxkAP_OQOTWmo6w</recordid><startdate>19990705</startdate><enddate>19990705</enddate><creator>Anderson, Christopher M</creator><creator>Baldwin, Stephen A</creator><creator>Young, James D</creator><creator>Cass, Carol E</creator><creator>Parkinson, Fiona E</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19990705</creationdate><title>Distribution of mRNA encoding a nitrobenzylthioinosine-insensitive nucleoside transporter (ENT2) in rat brain</title><author>Anderson, Christopher M ; Baldwin, Stephen A ; Young, James D ; Cass, Carol E ; Parkinson, Fiona E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-f8e8e4f55af067195963e5da6a9f9a73518a25c40a2ef3c25ba363e9be4e9913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine</topic><topic>Adenosine transport</topic><topic>Anatomy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Carrier Proteins - genetics</topic><topic>Central nervous system</topic><topic>Cerebrovascular Circulation - physiology</topic><topic>Choroid Plexus - metabolism</topic><topic>Ei nucleoside transporter</topic><topic>ENT2</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus - metabolism</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Membrane Proteins - genetics</topic><topic>Nitrobenzylthioinosine</topic><topic>Nucleoside Transport Proteins</topic><topic>Organ Specificity</topic><topic>Rats</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>RT-PCR</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, Christopher M</creatorcontrib><creatorcontrib>Baldwin, Stephen A</creatorcontrib><creatorcontrib>Young, James D</creatorcontrib><creatorcontrib>Cass, Carol E</creatorcontrib><creatorcontrib>Parkinson, Fiona E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anderson, Christopher M</au><au>Baldwin, Stephen A</au><au>Young, James D</au><au>Cass, Carol E</au><au>Parkinson, Fiona E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of mRNA encoding a nitrobenzylthioinosine-insensitive nucleoside transporter (ENT2) in rat brain</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1999-07-05</date><risdate>1999</risdate><volume>70</volume><issue>2</issue><spage>293</spage><epage>297</epage><pages>293-297</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>Nucleoside transporters may play a role in regulating levels of extracellular adenosine and adenosine receptor activity. Two members of the equilibrative nucleoside transporter family have recently been cloned. ENT1 is potently inhibited by nitrobenzylthioinosine (NBMPR) (
K
i
≈1 nM) and was previously found to have a wide distribution in rat and human brain. ENT2 is insensitive to inhibition by NBMPR at low nanomolar concentrations and there is limited information describing its distribution in rat brain. The present study used RT-PCR, northern blot and in situ hybridization and detected rENT2 transcript in several brain regions including hippocampus, cortex, striatum and cerebellum. Our results indicate a wide cellular and regional distribution for ENT2 in rat brain, similar to ENT1, indicating that control of adenosine levels in brain is achieved by multiple transport processes.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10407178</pmid><doi>10.1016/S0169-328X(99)00164-3</doi><tpages>5</tpages></addata></record> |
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| ispartof | Brain research. Molecular brain research., 1999-07, Vol.70 (2), p.293-297 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adenosine Adenosine transport Anatomy Animals Biological and medical sciences Brain - metabolism Carrier Proteins - genetics Central nervous system Cerebrovascular Circulation - physiology Choroid Plexus - metabolism Ei nucleoside transporter ENT2 Fundamental and applied biological sciences. Psychology Hippocampus - metabolism Humans In Situ Hybridization Membrane Proteins - genetics Nitrobenzylthioinosine Nucleoside Transport Proteins Organ Specificity Rats Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - genetics RT-PCR Transcription, Genetic Vertebrates: nervous system and sense organs |
| title | Distribution of mRNA encoding a nitrobenzylthioinosine-insensitive nucleoside transporter (ENT2) in rat brain |
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