Regulation of Osteoblast Levels During Bone Healing
OBJECTIVE:To confirm the occurrence of programmed cell death of osteoblasts during bone healing and to evaluate the role of interleukin-1β (IL-1β) in regulating osteoblast concentration. STUDY DESIGN:Electron microscopic study of the response of rats to a controlled bone injury, and a randomized con...
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| Veröffentlicht in: | Journal of orthopaedic trauma 1999-06, Vol.13 (5), p.356-362 |
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| container_title | Journal of orthopaedic trauma |
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| creator | Olmedo, Margaret L Landry, Patricia S Sadasivan, Kalia K Albright, James A Meek, William D Routh, Robert Marino, Andrew A |
| description | OBJECTIVE:To confirm the occurrence of programmed cell death of osteoblasts during bone healing and to evaluate the role of interleukin-1β (IL-1β) in regulating osteoblast concentration.
STUDY DESIGN:Electron microscopic study of the response of rats to a controlled bone injury, and a randomized controlled study of the effect of IL-1β administered continuously for three days.
METHODS:A standardized defect (1.1 millimeter in diameter, 0.5 millimeter deep) was created unilaterally on the anteromedial surface of the tibia. In some animals, the injury site was recovered five days after operation and processed for ultrastructural evaluation of osteoblasts in the callus. In another group, IL-1β was delivered to the bone defect using micro-osmotic pumps (0.5 nanograms/hour); control rats received vehicle only. The bones were recovered one to fourteen days after injury, and concentrations of proliferating cells, osteoblasts, and apoptotic bodies were determined. The amount of callus that formed in the defect was measured.
RESULTS:Osteoblasts in the callus exhibited ultrastructural changes characteristic of cells undergoing apoptosis, including condensation of chromatin, membrane blebbing, formation of apoptotic bodies, and phagocytosis by nearby osteoblasts. Addition of IL-1β significantly increased the number of osteoblasts at the injury site and significantly decreased the number of apoptotic bodies in relation to the number of osteoblasts. The amount of callus in the bone defect was not affected by IL-1β treatment.
CONCLUSION:The role of programmed cell death of osteoblasts as a normal concomitant of bone healing was confirmed. Evidence was found suggesting that IL-1β mediated the appearance and disappearance of osteoblasts, possibly by affecting the rates of differentiation and apoptosis, respectively. Understanding these mechanisms conceivably could lead to the ability to control osteoblast levels at an injury site. |
| doi_str_mv | 10.1097/00005131-199906000-00006 |
| format | Article |
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STUDY DESIGN:Electron microscopic study of the response of rats to a controlled bone injury, and a randomized controlled study of the effect of IL-1β administered continuously for three days.
METHODS:A standardized defect (1.1 millimeter in diameter, 0.5 millimeter deep) was created unilaterally on the anteromedial surface of the tibia. In some animals, the injury site was recovered five days after operation and processed for ultrastructural evaluation of osteoblasts in the callus. In another group, IL-1β was delivered to the bone defect using micro-osmotic pumps (0.5 nanograms/hour); control rats received vehicle only. The bones were recovered one to fourteen days after injury, and concentrations of proliferating cells, osteoblasts, and apoptotic bodies were determined. The amount of callus that formed in the defect was measured.
RESULTS:Osteoblasts in the callus exhibited ultrastructural changes characteristic of cells undergoing apoptosis, including condensation of chromatin, membrane blebbing, formation of apoptotic bodies, and phagocytosis by nearby osteoblasts. Addition of IL-1β significantly increased the number of osteoblasts at the injury site and significantly decreased the number of apoptotic bodies in relation to the number of osteoblasts. The amount of callus in the bone defect was not affected by IL-1β treatment.
CONCLUSION:The role of programmed cell death of osteoblasts as a normal concomitant of bone healing was confirmed. Evidence was found suggesting that IL-1β mediated the appearance and disappearance of osteoblasts, possibly by affecting the rates of differentiation and apoptosis, respectively. Understanding these mechanisms conceivably could lead to the ability to control osteoblast levels at an injury site.</description><identifier>ISSN: 0890-5339</identifier><identifier>EISSN: 1531-2291</identifier><identifier>DOI: 10.1097/00005131-199906000-00006</identifier><identifier>PMID: 10406703</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Animals ; Apoptosis ; Biological and medical sciences ; Bone and Bones - injuries ; Bone and Bones - pathology ; Degeneration. Regeneration. Wound healing. Graft ; Disease Models, Animal ; Fracture Healing - physiology ; Fractures, Bone - pathology ; Fundamental and applied biological sciences. Psychology ; Interleukin-1 - analysis ; Interleukin-1 - biosynthesis ; Male ; Osteoblasts - pathology ; Random Allocation ; Rats ; Rats, Inbred F344 ; Reference Values ; Sensitivity and Specificity ; Statistics, Nonparametric ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Journal of orthopaedic trauma, 1999-06, Vol.13 (5), p.356-362</ispartof><rights>1999 Lippincott Williams & Wilkins, Inc.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4146-17a90ef0d42739791d01f9e4f8b2a89e1fbd1b7559f45743e2260eb0bcb45e5d3</citedby><cites>FETCH-LOGICAL-c4146-17a90ef0d42739791d01f9e4f8b2a89e1fbd1b7559f45743e2260eb0bcb45e5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1864937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10406703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olmedo, Margaret L</creatorcontrib><creatorcontrib>Landry, Patricia S</creatorcontrib><creatorcontrib>Sadasivan, Kalia K</creatorcontrib><creatorcontrib>Albright, James A</creatorcontrib><creatorcontrib>Meek, William D</creatorcontrib><creatorcontrib>Routh, Robert</creatorcontrib><creatorcontrib>Marino, Andrew A</creatorcontrib><title>Regulation of Osteoblast Levels During Bone Healing</title><title>Journal of orthopaedic trauma</title><addtitle>J Orthop Trauma</addtitle><description>OBJECTIVE:To confirm the occurrence of programmed cell death of osteoblasts during bone healing and to evaluate the role of interleukin-1β (IL-1β) in regulating osteoblast concentration.
STUDY DESIGN:Electron microscopic study of the response of rats to a controlled bone injury, and a randomized controlled study of the effect of IL-1β administered continuously for three days.
METHODS:A standardized defect (1.1 millimeter in diameter, 0.5 millimeter deep) was created unilaterally on the anteromedial surface of the tibia. In some animals, the injury site was recovered five days after operation and processed for ultrastructural evaluation of osteoblasts in the callus. In another group, IL-1β was delivered to the bone defect using micro-osmotic pumps (0.5 nanograms/hour); control rats received vehicle only. The bones were recovered one to fourteen days after injury, and concentrations of proliferating cells, osteoblasts, and apoptotic bodies were determined. The amount of callus that formed in the defect was measured.
RESULTS:Osteoblasts in the callus exhibited ultrastructural changes characteristic of cells undergoing apoptosis, including condensation of chromatin, membrane blebbing, formation of apoptotic bodies, and phagocytosis by nearby osteoblasts. Addition of IL-1β significantly increased the number of osteoblasts at the injury site and significantly decreased the number of apoptotic bodies in relation to the number of osteoblasts. The amount of callus in the bone defect was not affected by IL-1β treatment.
CONCLUSION:The role of programmed cell death of osteoblasts as a normal concomitant of bone healing was confirmed. Evidence was found suggesting that IL-1β mediated the appearance and disappearance of osteoblasts, possibly by affecting the rates of differentiation and apoptosis, respectively. Understanding these mechanisms conceivably could lead to the ability to control osteoblast levels at an injury site.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Bone and Bones - injuries</subject><subject>Bone and Bones - pathology</subject><subject>Degeneration. Regeneration. Wound healing. Graft</subject><subject>Disease Models, Animal</subject><subject>Fracture Healing - physiology</subject><subject>Fractures, Bone - pathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Interleukin-1 - analysis</subject><subject>Interleukin-1 - biosynthesis</subject><subject>Male</subject><subject>Osteoblasts - pathology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Reference Values</subject><subject>Sensitivity and Specificity</subject><subject>Statistics, Nonparametric</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0890-5339</issn><issn>1531-2291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kclOwzAQhi0EoqXwCigHxC0wXrL4CGUpUqVKCM6Wk4zbgJsUO6Hi7XFpWS7MxZpf38xInwmJKFxQkNklhEoopzGVUkIaungTpXtkSJMQMybpPhlCLiFOOJcDcuT9SyByYOyQDCgISDPgQ8Ifcd5b3dVtE7UmmvkO28Jq30VTfEfro5ve1c08um4bjCaobWiOyYHR1uPJ7h2R57vbp_Ekns7uH8ZX07gUVKQxzbQENFAJlnGZSVoBNRKFyQumc4nUFBUtsiSRRiSZ4MhYClhAURYiwaTiI3K-3bty7VuPvlPL2pdorW6w7b1KZS4FAxrAfAuWrvXeoVErVy-1-1AU1EaY-hamfoR9RWkYPd3d6IslVn8Gt4YCcLYDtC-1NU43Ze1_uTwVkmcBE1ts3doOnX-1_RqdWgRj3UL991_8Ey-mgHA</recordid><startdate>199906</startdate><enddate>199906</enddate><creator>Olmedo, Margaret L</creator><creator>Landry, Patricia S</creator><creator>Sadasivan, Kalia K</creator><creator>Albright, James A</creator><creator>Meek, William D</creator><creator>Routh, Robert</creator><creator>Marino, Andrew A</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199906</creationdate><title>Regulation of Osteoblast Levels During Bone Healing</title><author>Olmedo, Margaret L ; Landry, Patricia S ; Sadasivan, Kalia K ; Albright, James A ; Meek, William D ; Routh, Robert ; Marino, Andrew A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4146-17a90ef0d42739791d01f9e4f8b2a89e1fbd1b7559f45743e2260eb0bcb45e5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Bone and Bones - injuries</topic><topic>Bone and Bones - pathology</topic><topic>Degeneration. Regeneration. Wound healing. Graft</topic><topic>Disease Models, Animal</topic><topic>Fracture Healing - physiology</topic><topic>Fractures, Bone - pathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Interleukin-1 - analysis</topic><topic>Interleukin-1 - biosynthesis</topic><topic>Male</topic><topic>Osteoblasts - pathology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Reference Values</topic><topic>Sensitivity and Specificity</topic><topic>Statistics, Nonparametric</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olmedo, Margaret L</creatorcontrib><creatorcontrib>Landry, Patricia S</creatorcontrib><creatorcontrib>Sadasivan, Kalia K</creatorcontrib><creatorcontrib>Albright, James A</creatorcontrib><creatorcontrib>Meek, William D</creatorcontrib><creatorcontrib>Routh, Robert</creatorcontrib><creatorcontrib>Marino, Andrew A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic trauma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olmedo, Margaret L</au><au>Landry, Patricia S</au><au>Sadasivan, Kalia K</au><au>Albright, James A</au><au>Meek, William D</au><au>Routh, Robert</au><au>Marino, Andrew A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Osteoblast Levels During Bone Healing</atitle><jtitle>Journal of orthopaedic trauma</jtitle><addtitle>J Orthop Trauma</addtitle><date>1999-06</date><risdate>1999</risdate><volume>13</volume><issue>5</issue><spage>356</spage><epage>362</epage><pages>356-362</pages><issn>0890-5339</issn><eissn>1531-2291</eissn><abstract>OBJECTIVE:To confirm the occurrence of programmed cell death of osteoblasts during bone healing and to evaluate the role of interleukin-1β (IL-1β) in regulating osteoblast concentration.
STUDY DESIGN:Electron microscopic study of the response of rats to a controlled bone injury, and a randomized controlled study of the effect of IL-1β administered continuously for three days.
METHODS:A standardized defect (1.1 millimeter in diameter, 0.5 millimeter deep) was created unilaterally on the anteromedial surface of the tibia. In some animals, the injury site was recovered five days after operation and processed for ultrastructural evaluation of osteoblasts in the callus. In another group, IL-1β was delivered to the bone defect using micro-osmotic pumps (0.5 nanograms/hour); control rats received vehicle only. The bones were recovered one to fourteen days after injury, and concentrations of proliferating cells, osteoblasts, and apoptotic bodies were determined. The amount of callus that formed in the defect was measured.
RESULTS:Osteoblasts in the callus exhibited ultrastructural changes characteristic of cells undergoing apoptosis, including condensation of chromatin, membrane blebbing, formation of apoptotic bodies, and phagocytosis by nearby osteoblasts. Addition of IL-1β significantly increased the number of osteoblasts at the injury site and significantly decreased the number of apoptotic bodies in relation to the number of osteoblasts. The amount of callus in the bone defect was not affected by IL-1β treatment.
CONCLUSION:The role of programmed cell death of osteoblasts as a normal concomitant of bone healing was confirmed. Evidence was found suggesting that IL-1β mediated the appearance and disappearance of osteoblasts, possibly by affecting the rates of differentiation and apoptosis, respectively. Understanding these mechanisms conceivably could lead to the ability to control osteoblast levels at an injury site.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>10406703</pmid><doi>10.1097/00005131-199906000-00006</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of orthopaedic trauma, 1999-06, Vol.13 (5), p.356-362 |
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| language | eng |
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| subjects | Animals Apoptosis Biological and medical sciences Bone and Bones - injuries Bone and Bones - pathology Degeneration. Regeneration. Wound healing. Graft Disease Models, Animal Fracture Healing - physiology Fractures, Bone - pathology Fundamental and applied biological sciences. Psychology Interleukin-1 - analysis Interleukin-1 - biosynthesis Male Osteoblasts - pathology Random Allocation Rats Rats, Inbred F344 Reference Values Sensitivity and Specificity Statistics, Nonparametric Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Regulation of Osteoblast Levels During Bone Healing |
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