A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region
Abstract The analysis of X-STR polymorphisms has received the attention of several researchers, mainly due to its applicability to the investigation of complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in dif...
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| Veröffentlicht in: | Forensic science international : genetics 2008-03, Vol.2 (2), p.154-158 |
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| creator | Ribeiro Rodrigues, Elzemar Martins Leite, Fabio Pereira das Neves Hutz, Mara Helena Palha, Teresinha de Jesus Brabo Ferreira Ribeiro dos Santos, Ândrea Kely Campos dos Santos, Sidney Emanuel Batista |
| description | Abstract The analysis of X-STR polymorphisms has received the attention of several researchers, mainly due to its applicability to the investigation of complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in different populations of the world. The present work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. The loci investigated include DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011, which together afford a power of discrimination in the order of 0.999999. In addition, this work presents the genotyping results obtained for a sample of 324 individuals (182 males and 142 females) from the admixed population of Belém, Pará, located in the Brazilian Amazon Region. |
| doi_str_mv | 10.1016/j.fsigen.2007.10.179 |
| format | Article |
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Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in different populations of the world. The present work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. The loci investigated include DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011, which together afford a power of discrimination in the order of 0.999999. In addition, this work presents the genotyping results obtained for a sample of 324 individuals (182 males and 142 females) from the admixed population of Belém, Pará, located in the Brazilian Amazon Region.</description><identifier>ISSN: 1872-4973</identifier><identifier>EISSN: 1878-0326</identifier><identifier>DOI: 10.1016/j.fsigen.2007.10.179</identifier><identifier>PMID: 19083811</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Brazil ; Chromosomes, Human, X ; Female ; Forensic Genetics ; Genetic Markers ; Genetics, Population ; Humans ; Male ; Microsatellite Repeats - genetics ; Paternity ; Pathology ; Polymerase Chain Reaction ; Rivers</subject><ispartof>Forensic science international : genetics, 2008-03, Vol.2 (2), p.154-158</ispartof><rights>Elsevier Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-5645e6029fa7d706148df6ea410344d91705d55f716c85d68cfdca21f7a99dde3</citedby><cites>FETCH-LOGICAL-c426t-5645e6029fa7d706148df6ea410344d91705d55f716c85d68cfdca21f7a99dde3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19083811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ribeiro Rodrigues, Elzemar Martins</creatorcontrib><creatorcontrib>Leite, Fabio Pereira das Neves</creatorcontrib><creatorcontrib>Hutz, Mara Helena</creatorcontrib><creatorcontrib>Palha, Teresinha de Jesus Brabo Ferreira</creatorcontrib><creatorcontrib>Ribeiro dos Santos, Ândrea Kely Campos</creatorcontrib><creatorcontrib>dos Santos, Sidney Emanuel Batista</creatorcontrib><title>A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region</title><title>Forensic science international : genetics</title><addtitle>Forensic Sci Int Genet</addtitle><description>Abstract The analysis of X-STR polymorphisms has received the attention of several researchers, mainly due to its applicability to the investigation of complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variations of these polymorphisms in different populations of the world. The present work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. The loci investigated include DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011, which together afford a power of discrimination in the order of 0.999999. 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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Brazil Chromosomes, Human, X Female Forensic Genetics Genetic Markers Genetics, Population Humans Male Microsatellite Repeats - genetics Paternity Pathology Polymerase Chain Reaction Rivers |
| title | A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region |
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