Multiple Splicing Variants of cdc25B Regulate G2/M Progression
Progression through G2 phase into mitosis is regulated by the activation of the mitotic cyclin/cdk complexes, which are in turn activated cdc25B and cdc25C phosphatases. Here we report that alternate splicing produces at least five variants of cdc25B, although only cdc25B2 and cdc25B3 are detectable...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1999-07, Vol.260 (2), p.510-515 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Forrest, A.R.R. McCormack, A.K. DeSouza, C.P.C. Sinnamon, J.M. Tonks, I.D. Hayward, N.K. Ellem, K.A.O. Gabrielli, B.G. |
| description | Progression through G2 phase into mitosis is regulated by the activation of the mitotic cyclin/cdk complexes, which are in turn activated cdc25B and cdc25C phosphatases. Here we report that alternate splicing produces at least five variants of cdc25B, although only cdc25B2 and cdc25B3 are detectable as proteins. Analysis of these two variants shows that cdc25B2 is expressed at lower levels relative to cdc25B3 in all cell lines tested, and the expression of both increased markedly during G2 and mitosis. Overexpression of the catalytically inactive version of either cdc25B variant produced a G2 arrest implicating both in regulating G2/M progression. |
| doi_str_mv | 10.1006/bbrc.1999.0870 |
| format | Article |
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Overexpression of the catalytically inactive version of either cdc25B variant produced a G2 arrest implicating both in regulating G2/M progression.</description><subject>cdc25 Phosphatases</subject><subject>Cell Cycle Proteins - chemistry</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - physiology</subject><subject>Cell Line</subject><subject>G2 Phase - genetics</subject><subject>Humans</subject><subject>Mitosis - genetics</subject><subject>Molecular Weight</subject><subject>Phosphoprotein Phosphatases - chemistry</subject><subject>Phosphoprotein Phosphatases - genetics</subject><subject>Phosphoprotein Phosphatases - physiology</subject><subject>Protein Isoforms - chemistry</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - physiology</subject><subject>RNA Splicing</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0L9LxDAYxvEgineero7Sya01SdM0WQQ99BTuUPyFW0iTt0ek19akFfzvbbkbXMQpQz7vM3wROiU4IRjzi6LwJiFSygSLHO-hKcESx5Rgto-meBAxleR9go5C-MCYEMblIZoM3zjNpZiiy1Vfda6tIHpuK2dcvY7etHe67kLUlJGxhmbX0ROs-0p3EC3oxSp69M3aQwiuqY_RQamrACe7d4Zeb29e5nfx8mFxP79axoaleRczDtaWMqOYaJEbqqVklhagTWmMNAUvLaMDMZSnVgLWggEXTIqUl4zRNJ2h8-1u65vPHkKnNi4YqCpdQ9MHxaWQKc_yfyHJaZZhLgaYbKHxTQgeStV6t9H-WxGsxrRqTKvGtGpMOxyc7Zb7YgP2F9-2HIDYAhhCfDnwKhgHtQHrPJhO2cb9tf0DuNSG-Q</recordid><startdate>19990705</startdate><enddate>19990705</enddate><creator>Forrest, A.R.R.</creator><creator>McCormack, A.K.</creator><creator>DeSouza, C.P.C.</creator><creator>Sinnamon, J.M.</creator><creator>Tonks, I.D.</creator><creator>Hayward, N.K.</creator><creator>Ellem, K.A.O.</creator><creator>Gabrielli, B.G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19990705</creationdate><title>Multiple Splicing Variants of cdc25B Regulate G2/M Progression</title><author>Forrest, A.R.R. ; McCormack, A.K. ; DeSouza, C.P.C. ; Sinnamon, J.M. ; Tonks, I.D. ; Hayward, N.K. ; Ellem, K.A.O. ; Gabrielli, B.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-46eddf95201a87c2a994d2beacfcc9cb6fd42eddc263d9e0a84e6849836f44233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>cdc25 Phosphatases</topic><topic>Cell Cycle Proteins - chemistry</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - physiology</topic><topic>Cell Line</topic><topic>G2 Phase - genetics</topic><topic>Humans</topic><topic>Mitosis - genetics</topic><topic>Molecular Weight</topic><topic>Phosphoprotein Phosphatases - chemistry</topic><topic>Phosphoprotein Phosphatases - genetics</topic><topic>Phosphoprotein Phosphatases - physiology</topic><topic>Protein Isoforms - chemistry</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - physiology</topic><topic>RNA Splicing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forrest, A.R.R.</creatorcontrib><creatorcontrib>McCormack, A.K.</creatorcontrib><creatorcontrib>DeSouza, C.P.C.</creatorcontrib><creatorcontrib>Sinnamon, J.M.</creatorcontrib><creatorcontrib>Tonks, I.D.</creatorcontrib><creatorcontrib>Hayward, N.K.</creatorcontrib><creatorcontrib>Ellem, K.A.O.</creatorcontrib><creatorcontrib>Gabrielli, B.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forrest, A.R.R.</au><au>McCormack, A.K.</au><au>DeSouza, C.P.C.</au><au>Sinnamon, J.M.</au><au>Tonks, I.D.</au><au>Hayward, N.K.</au><au>Ellem, K.A.O.</au><au>Gabrielli, B.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiple Splicing Variants of cdc25B Regulate G2/M Progression</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1999-07-05</date><risdate>1999</risdate><volume>260</volume><issue>2</issue><spage>510</spage><epage>515</epage><pages>510-515</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Progression through G2 phase into mitosis is regulated by the activation of the mitotic cyclin/cdk complexes, which are in turn activated cdc25B and cdc25C phosphatases. 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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | cdc25 Phosphatases Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics Cell Cycle Proteins - physiology Cell Line G2 Phase - genetics Humans Mitosis - genetics Molecular Weight Phosphoprotein Phosphatases - chemistry Phosphoprotein Phosphatases - genetics Phosphoprotein Phosphatases - physiology Protein Isoforms - chemistry Protein Isoforms - genetics Protein Isoforms - physiology RNA Splicing |
| title | Multiple Splicing Variants of cdc25B Regulate G2/M Progression |
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