Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase
Kinetic microplate-based assays for both mitochondrial NADH:ubiquinone oxidoreductase (complex I) and succinate:cytochrome c oxidoreductase using insect submitochondrial particles as the source of the enzyme activities have been developed. These assays have been used to design high-throughput screen...
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| Veröffentlicht in: | Analytical biochemistry 1999-07, Vol.272 (1), p.56-63 |
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| container_title | Analytical biochemistry |
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| creator | Jewess, P.J. Devonshire, A.L. |
| description | Kinetic microplate-based assays for both mitochondrial NADH:ubiquinone oxidoreductase (complex I) and succinate:cytochrome c oxidoreductase using insect submitochondrial particles as the source of the enzyme activities have been developed. These assays have been used to design high-throughput screens for inhibitors of these mitochondrial electron transfer activities to assess their intrinsic in vitro efficacies as potential pesticides. These methods can be used to test up to 60 compounds per day without the use of automated sample handling and diluting technology. The accuracy, specificity, and reproducibility of the microplate methods compared well with conventional spectrophotometer-based assays. |
| doi_str_mv | 10.1006/abio.1999.4168 |
| format | Article |
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These assays have been used to design high-throughput screens for inhibitors of these mitochondrial electron transfer activities to assess their intrinsic in vitro efficacies as potential pesticides. These methods can be used to test up to 60 compounds per day without the use of automated sample handling and diluting technology. The accuracy, specificity, and reproducibility of the microplate methods compared well with conventional spectrophotometer-based assays.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.1999.4168</identifier><identifier>PMID: 10405293</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>96-well ; Animals ; assay ; complex I ; complex III ; Diptera - enzymology ; Drug Evaluation, Preclinical ; Enzyme Inhibitors - analysis ; Evaluation Studies as Topic ; In Vitro Techniques ; insect ; Kinetics ; microplate ; mitochondria ; Mitochondria, Muscle - enzymology ; NAD(P)H Dehydrogenase (Quinone) - antagonists & inhibitors ; NADH ; Reproducibility of Results ; Spectrophotometry ; Submitochondrial Particles - enzymology ; Succinate Cytochrome c Oxidoreductase - antagonists & inhibitors</subject><ispartof>Analytical biochemistry, 1999-07, Vol.272 (1), p.56-63</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-2ee3243dfca09ec791587616441876acbe7e08e226215c1df96f6a5c575a9ccb3</citedby><cites>FETCH-LOGICAL-c340t-2ee3243dfca09ec791587616441876acbe7e08e226215c1df96f6a5c575a9ccb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003269799941684$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10405293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jewess, P.J.</creatorcontrib><creatorcontrib>Devonshire, A.L.</creatorcontrib><title>Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Kinetic microplate-based assays for both mitochondrial NADH:ubiquinone oxidoreductase (complex I) and succinate:cytochrome c oxidoreductase using insect submitochondrial particles as the source of the enzyme activities have been developed. These assays have been used to design high-throughput screens for inhibitors of these mitochondrial electron transfer activities to assess their intrinsic in vitro efficacies as potential pesticides. These methods can be used to test up to 60 compounds per day without the use of automated sample handling and diluting technology. The accuracy, specificity, and reproducibility of the microplate methods compared well with conventional spectrophotometer-based assays.</description><subject>96-well</subject><subject>Animals</subject><subject>assay</subject><subject>complex I</subject><subject>complex III</subject><subject>Diptera - enzymology</subject><subject>Drug Evaluation, Preclinical</subject><subject>Enzyme Inhibitors - analysis</subject><subject>Evaluation Studies as Topic</subject><subject>In Vitro Techniques</subject><subject>insect</subject><subject>Kinetics</subject><subject>microplate</subject><subject>mitochondria</subject><subject>Mitochondria, Muscle - enzymology</subject><subject>NAD(P)H Dehydrogenase (Quinone) - antagonists & inhibitors</subject><subject>NADH</subject><subject>Reproducibility of Results</subject><subject>Spectrophotometry</subject><subject>Submitochondrial Particles - enzymology</subject><subject>Succinate Cytochrome c Oxidoreductase - antagonists & inhibitors</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1P3DAQhi1UVLa0V46VT1V7yGLnw4m5bbdQVkA5tJwtZzwRrhJ7sZOK_R_8YBwthwqJw2guzzyjmZeQE86WnDFxqlvrl1xKuSy5aA7IgjMpMlYw-Y4sGGNFlgtZH5EPMf5ljPOyEu_JEWclq3JZLMjTlXU4WqA3FoLf9nrE7LuOaOgqRr2LtPOBbty9be3oQ6S-S-To4d47E6zu6a_Vj8uzu9Y-TNZ5h_T20Rof0EwwJg39uvbDtsdHuvlGtTP09wRgXdpytt7NmuAHpPBq6iM57HQf8dNLPyZ3F-d_1pfZ9e3PzXp1nUFRsjHLEYu8LEwHmkmEWvKqqQUXZclT19BijazBPBc5r4CbTopO6AqqutISoC2OyZe9dxv8w4RxVIONgH2vHfopKiEbyVMlcLkH049iDNipbbCDDjvFmZpzUHMOas5BzTmkgc8v5qkd0PyH7x-fgGYPYLrvn8WgIlh0gMYGhFEZb99yPwNnHpmU</recordid><startdate>19990715</startdate><enddate>19990715</enddate><creator>Jewess, P.J.</creator><creator>Devonshire, A.L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990715</creationdate><title>Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase</title><author>Jewess, P.J. ; Devonshire, A.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-2ee3243dfca09ec791587616441876acbe7e08e226215c1df96f6a5c575a9ccb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>96-well</topic><topic>Animals</topic><topic>assay</topic><topic>complex I</topic><topic>complex III</topic><topic>Diptera - enzymology</topic><topic>Drug Evaluation, Preclinical</topic><topic>Enzyme Inhibitors - analysis</topic><topic>Evaluation Studies as Topic</topic><topic>In Vitro Techniques</topic><topic>insect</topic><topic>Kinetics</topic><topic>microplate</topic><topic>mitochondria</topic><topic>Mitochondria, Muscle - enzymology</topic><topic>NAD(P)H Dehydrogenase (Quinone) - antagonists & inhibitors</topic><topic>NADH</topic><topic>Reproducibility of Results</topic><topic>Spectrophotometry</topic><topic>Submitochondrial Particles - enzymology</topic><topic>Succinate Cytochrome c Oxidoreductase - antagonists & inhibitors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jewess, P.J.</creatorcontrib><creatorcontrib>Devonshire, A.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jewess, P.J.</au><au>Devonshire, A.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1999-07-15</date><risdate>1999</risdate><volume>272</volume><issue>1</issue><spage>56</spage><epage>63</epage><pages>56-63</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Kinetic microplate-based assays for both mitochondrial NADH:ubiquinone oxidoreductase (complex I) and succinate:cytochrome c oxidoreductase using insect submitochondrial particles as the source of the enzyme activities have been developed. These assays have been used to design high-throughput screens for inhibitors of these mitochondrial electron transfer activities to assess their intrinsic in vitro efficacies as potential pesticides. These methods can be used to test up to 60 compounds per day without the use of automated sample handling and diluting technology. The accuracy, specificity, and reproducibility of the microplate methods compared well with conventional spectrophotometer-based assays.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10405293</pmid><doi>10.1006/abio.1999.4168</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1999-07, Vol.272 (1), p.56-63 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69891989 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 96-well Animals assay complex I complex III Diptera - enzymology Drug Evaluation, Preclinical Enzyme Inhibitors - analysis Evaluation Studies as Topic In Vitro Techniques insect Kinetics microplate mitochondria Mitochondria, Muscle - enzymology NAD(P)H Dehydrogenase (Quinone) - antagonists & inhibitors NADH Reproducibility of Results Spectrophotometry Submitochondrial Particles - enzymology Succinate Cytochrome c Oxidoreductase - antagonists & inhibitors |
| title | Kinetic Microplate-Based Assays for Inhibitors of Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I) and Succinate:Cytochrome c Oxidoreductase |
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