A bacterial single-chain Fv antibody fragment that inhibits binding of its parental anti- E-Selectin monoclonal antibody to activated human endothelial cells

Using the polymerase chain reaction, we cloned, modified, and linked antibody variable (V) region coding genes from a mouse hybridoma, and produced a bacterial single-chain Fv (scFv) antibody fragment specific for E-Selectin. A vector of pBR322 origin, bearing the tryptophan promoter and the ompA ba...

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Veröffentlicht in:	Journal of biotechnology 1999-06, Vol.72 (1), p.1-12
Hauptverfasser:	Casalvilla, Racmar, Dueñas, Marta, Ayala, Marta, Cruz, Silian, Cruz, Liset, Buurman, Wim A., Gavilondo, Jorge V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antibody fragments Bacteria Base Sequence Binding Sites, Antibody Bioassay Biological and medical sciences Biotechnology Cells, Cultured Cloning, Molecular DNA, Recombinant E-Selectin E-Selectin - immunology Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - immunology Enzyme-Linked Immunosorbent Assay Enzymes Escherichia coli - genetics Fractionation Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Humans Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Industrial applications and implications. Economical aspects Mice Molecular Sequence Data Monoclonal antibodies Polymerase Chain Reaction Production of active biomolecules Recombinant Proteins - genetics Recombinant Proteins - immunology ScFv Solubility Transmission electron microscopy Tumor Necrosis Factor-alpha - pharmacology
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