Membrane Binding by tBid Initiates an Ordered Series of Events Culminating in Membrane Permeabilization by Bax
In normal circumstances, the Bcl-2 family dutifully governs when cells die. However, the rules of engagement between the pro- and antiapoptotic family members are still contested, and how Bax is transformed from a cytosolic monomer to an outer mitochondrial membrane-permeabilizing oligomer is unclea...
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| Veröffentlicht in: | Cell 2008-12, Vol.135 (6), p.1074-1084 |
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| container_issue | 6 |
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| container_title | Cell |
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| creator | Lovell, Jonathan F. Billen, Lieven P. Bindner, Scott Shamas-Din, Aisha Fradin, Cecile Leber, Brian Andrews, David W. |
| description | In normal circumstances, the Bcl-2 family dutifully governs when cells die. However, the rules of engagement between the pro- and antiapoptotic family members are still contested, and how Bax is transformed from a cytosolic monomer to an outer mitochondrial membrane-permeabilizing oligomer is unclear. With fluorescence techniques and an in vitro system, the combination of tBid and Bax produced dramatic membrane permeabilization. The membrane is not a passive partner in this process beause membranes are required for the protein-protein interactions to occur. Simultaneous measurements of these interactions revealed an ordered series of steps required for outer membrane permeabilization: (1) tBid rapidly binds to membranes, where (2) tBid interacts with Bax, causing (3) Bax insertion into membranes and (4) oligomerization, culminating in (5) membrane permeabilization. Bcl-XL prevents membrane-bound tBid from binding Bax. Bad releases tBid from Bcl-XL, restoring both tBid binding to Bax and membrane permeabilization. |
| doi_str_mv | 10.1016/j.cell.2008.11.010 |
| format | Article |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Apoptosis bcl-2-Associated X Protein - metabolism BH3 Interacting Domain Death Agonist Protein - metabolism Cattle CELLBIO Liposomes - metabolism Mitochondrial Membranes - metabolism SIGNALING |
| title | Membrane Binding by tBid Initiates an Ordered Series of Events Culminating in Membrane Permeabilization by Bax |
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