Analysis of the in vivo interaction between a basic repressor and an acidic activator
The artificial basic repressor SSB24 represses transcription of a reporter construct activated by GCN4. We show that the positively charged SSB24 and the negatively charged acidic activator GCN4 interact in vitro and in vivo. However, deleting the interaction domain from the GCN4 activator does not...
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| Veröffentlicht in: | FEBS letters 1999-06, Vol.453 (3), p.299-304 |
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| container_title | FEBS letters |
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| creator | Wellhausen, Anne Lehming, Norbert |
| description | The artificial basic repressor SSB24 represses transcription of a reporter construct activated by GCN4. We show that the positively charged SSB24 and the negatively charged acidic activator GCN4 interact in vitro and in vivo. However, deleting the interaction domain from the GCN4 activator does not result in loss of repression by SSB24. Similarly, transcription activated by the holoenzyme component SRB2 is repressed, although SSB24 and SRB2 do not interact. Repression by SSB24 therefore does not depend on the observed protein-protein interaction between SSB24 and GCN4. |
| doi_str_mv | 10.1016/S0014-5793(99)00718-8 |
| format | Article |
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| ispartof | FEBS letters, 1999-06, Vol.453 (3), p.299-304 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acids - metabolism Alkalies - metabolism DNA Mutational Analysis DNA-Binding Proteins Fungal Proteins - metabolism GCN4 Gene Expression Regulation Mediator Complex Protein Binding Protein Kinases - metabolism Recombinant Proteins Repression Repressor Proteins - metabolism Saccharomyces cerevisiae Proteins Sequence Deletion Split ubiquitin assay SSB24 Trans-Activators - metabolism Transcription Factors - metabolism Ubiquitins - metabolism |
| title | Analysis of the in vivo interaction between a basic repressor and an acidic activator |
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