Mother-to-infant transmission of hepatitis C virus: molecular evidence of superinfection by homologous virus in children
Background/Aim: Vertical transmission of hepatitis C virus (HCV) is well established but its incidence is low. To assess the molecular evidence of mother-to-infant transmission or intrafamilial transmission of HCV, the NS5 B region and the hypervariable region 1 (HVR1) of the E2/NS1 region of the HC...
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| Veröffentlicht in: | Journal of hepatology 1999-06, Vol.30 (6), p.970-978 |
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| container_title | Journal of hepatology |
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| creator | Halfon, Philippe Quentin, Yves Roquelaure, Bertrand Sarles, Jacques Halimi, Gilles Gerolami, Victoria Khiri, Hascene Bourlière, Marc Cartouzou, Guy |
| description | Background/Aim: Vertical transmission of hepatitis C virus (HCV) is well established but its incidence is low. To assess the molecular evidence of mother-to-infant transmission or intrafamilial transmission of HCV, the NS5 B region and the hypervariable region 1 (HVR1) of the E2/NS1 region of the HCV genome from each member of a family were investigated.
Methods: A 35-year-old mother with chronic hepatitis C virus infection and her four infected boys were studied. The same HCV 1a genotype was found in all five. Phylogenetic analysis was done by the neighbor-joining, the maximum likelihood, and the maximum parsimony methods.
Results: Comparison of the phylogenetic trees in the NS5B and HVR1 regions showed that the sequences in the children were more closely related to the population of variants of their own mother than to any genotype 1a sequence available in the databases. However, four HVR1 clones from two brothers (E2 and E3) had a strong homology, but were significantly divergent from the variants of the mother.
Conclusions: These results suggest that a cluster of HCV strains exists in the family and that E3 could have been superinfected by E2 HCV strains and reciprocally. In conclusion, phylogenetic analysis through variable regions of the genome suggests that at least two modes of transmission are involved in this family: perinatal and horizontal. |
| doi_str_mv | 10.1016/S0168-8278(99)80248-7 |
| format | Article |
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Methods: A 35-year-old mother with chronic hepatitis C virus infection and her four infected boys were studied. The same HCV 1a genotype was found in all five. Phylogenetic analysis was done by the neighbor-joining, the maximum likelihood, and the maximum parsimony methods.
Results: Comparison of the phylogenetic trees in the NS5B and HVR1 regions showed that the sequences in the children were more closely related to the population of variants of their own mother than to any genotype 1a sequence available in the databases. However, four HVR1 clones from two brothers (E2 and E3) had a strong homology, but were significantly divergent from the variants of the mother.
Conclusions: These results suggest that a cluster of HCV strains exists in the family and that E3 could have been superinfected by E2 HCV strains and reciprocally. In conclusion, phylogenetic analysis through variable regions of the genome suggests that at least two modes of transmission are involved in this family: perinatal and horizontal.</description><identifier>ISSN: 0168-8278</identifier><identifier>EISSN: 1600-0641</identifier><identifier>DOI: 10.1016/S0168-8278(99)80248-7</identifier><identifier>PMID: 10406172</identifier><identifier>CODEN: JOHEEC</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Adult ; Biological and medical sciences ; Child ; Child, Preschool ; Disease Transmission, Infectious ; Female ; Hepatitis B virus - genetics ; Hepatitis C virus ; Hepatitis C, Chronic - etiology ; Hepatitis C, Chronic - virology ; Human viral diseases ; Humans ; Infectious Disease Transmission, Vertical ; Infectious diseases ; Intrafamilial transmission ; Male ; Medical sciences ; Mother-to-infant transmission ; Quasispecies ; RNA, Viral - analysis ; Sequence Homology ; Superinfection ; Viral diseases ; Viral hepatitis</subject><ispartof>Journal of hepatology, 1999-06, Vol.30 (6), p.970-978</ispartof><rights>1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-fd7d8ff3eb1c7a882dfd2d4eb7bd9791b65537b63c172fc86c7017a485c1f6243</citedby><cites>FETCH-LOGICAL-c390t-fd7d8ff3eb1c7a882dfd2d4eb7bd9791b65537b63c172fc86c7017a485c1f6243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168827899802487$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1804976$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10406172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halfon, Philippe</creatorcontrib><creatorcontrib>Quentin, Yves</creatorcontrib><creatorcontrib>Roquelaure, Bertrand</creatorcontrib><creatorcontrib>Sarles, Jacques</creatorcontrib><creatorcontrib>Halimi, Gilles</creatorcontrib><creatorcontrib>Gerolami, Victoria</creatorcontrib><creatorcontrib>Khiri, Hascene</creatorcontrib><creatorcontrib>Bourlière, Marc</creatorcontrib><creatorcontrib>Cartouzou, Guy</creatorcontrib><title>Mother-to-infant transmission of hepatitis C virus: molecular evidence of superinfection by homologous virus in children</title><title>Journal of hepatology</title><addtitle>J Hepatol</addtitle><description>Background/Aim: Vertical transmission of hepatitis C virus (HCV) is well established but its incidence is low. To assess the molecular evidence of mother-to-infant transmission or intrafamilial transmission of HCV, the NS5 B region and the hypervariable region 1 (HVR1) of the E2/NS1 region of the HCV genome from each member of a family were investigated.
Methods: A 35-year-old mother with chronic hepatitis C virus infection and her four infected boys were studied. The same HCV 1a genotype was found in all five. Phylogenetic analysis was done by the neighbor-joining, the maximum likelihood, and the maximum parsimony methods.
Results: Comparison of the phylogenetic trees in the NS5B and HVR1 regions showed that the sequences in the children were more closely related to the population of variants of their own mother than to any genotype 1a sequence available in the databases. However, four HVR1 clones from two brothers (E2 and E3) had a strong homology, but were significantly divergent from the variants of the mother.
Conclusions: These results suggest that a cluster of HCV strains exists in the family and that E3 could have been superinfected by E2 HCV strains and reciprocally. In conclusion, phylogenetic analysis through variable regions of the genome suggests that at least two modes of transmission are involved in this family: perinatal and horizontal.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Disease Transmission, Infectious</subject><subject>Female</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis C virus</subject><subject>Hepatitis C, Chronic - etiology</subject><subject>Hepatitis C, Chronic - virology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious Disease Transmission, Vertical</subject><subject>Infectious diseases</subject><subject>Intrafamilial transmission</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mother-to-infant transmission</subject><subject>Quasispecies</subject><subject>RNA, Viral - analysis</subject><subject>Sequence Homology</subject><subject>Superinfection</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0168-8278</issn><issn>1600-0641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAURq0K1A6lP6HIC4RgEbATjx_doGrUAlIRC2BtOfY1Y5SJB9sZ0X9fpxkBOzb3bs53HwehS0reUkL5u6-1yEa2Qr5W6o0kLZONOEEryglpCGf0CVr9Qc7Qs5x_EkI6otgpOqOEEU5Fu0K_P8eyhdSU2ITRm7HgksyYdyHnEEccPd7C3pRQQsYbfAhpyld4Fwew02AShkNwMFqYwTztIdUhYMsc7e_xNlYy_ohTXpI4jNhuw-ASjM_RU2-GDBfHfo6-395823xs7r58-LS5vmtsp0hpvBNOet9BT60wUrbOu9Yx6EXvlFC05-t1J3re2fqOt5JbQagwTK4t9bxl3Tl6tczdp_hrglx0_c3CMJgR6mGaKykFa3kF1wtoU8w5gdf7FHYm3WtK9KxcPyrXs0-tlH5UrkXNvTgumPoduH9Si-MKvDwCJlsz-OrXhvyXk4QpMe9_v2BQbRwCJJ1tmN26kKpS7WL4zyUP53GgiA</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Halfon, Philippe</creator><creator>Quentin, Yves</creator><creator>Roquelaure, Bertrand</creator><creator>Sarles, Jacques</creator><creator>Halimi, Gilles</creator><creator>Gerolami, Victoria</creator><creator>Khiri, Hascene</creator><creator>Bourlière, Marc</creator><creator>Cartouzou, Guy</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Mother-to-infant transmission of hepatitis C virus: molecular evidence of superinfection by homologous virus in children</title><author>Halfon, Philippe ; Quentin, Yves ; Roquelaure, Bertrand ; Sarles, Jacques ; Halimi, Gilles ; Gerolami, Victoria ; Khiri, Hascene ; Bourlière, Marc ; Cartouzou, Guy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-fd7d8ff3eb1c7a882dfd2d4eb7bd9791b65537b63c172fc86c7017a485c1f6243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Disease Transmission, Infectious</topic><topic>Female</topic><topic>Hepatitis B virus - genetics</topic><topic>Hepatitis C virus</topic><topic>Hepatitis C, Chronic - etiology</topic><topic>Hepatitis C, Chronic - virology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious Disease Transmission, Vertical</topic><topic>Infectious diseases</topic><topic>Intrafamilial transmission</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mother-to-infant transmission</topic><topic>Quasispecies</topic><topic>RNA, Viral - analysis</topic><topic>Sequence Homology</topic><topic>Superinfection</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halfon, Philippe</creatorcontrib><creatorcontrib>Quentin, Yves</creatorcontrib><creatorcontrib>Roquelaure, Bertrand</creatorcontrib><creatorcontrib>Sarles, Jacques</creatorcontrib><creatorcontrib>Halimi, Gilles</creatorcontrib><creatorcontrib>Gerolami, Victoria</creatorcontrib><creatorcontrib>Khiri, Hascene</creatorcontrib><creatorcontrib>Bourlière, Marc</creatorcontrib><creatorcontrib>Cartouzou, Guy</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halfon, Philippe</au><au>Quentin, Yves</au><au>Roquelaure, Bertrand</au><au>Sarles, Jacques</au><au>Halimi, Gilles</au><au>Gerolami, Victoria</au><au>Khiri, Hascene</au><au>Bourlière, Marc</au><au>Cartouzou, Guy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mother-to-infant transmission of hepatitis C virus: molecular evidence of superinfection by homologous virus in children</atitle><jtitle>Journal of hepatology</jtitle><addtitle>J Hepatol</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>30</volume><issue>6</issue><spage>970</spage><epage>978</epage><pages>970-978</pages><issn>0168-8278</issn><eissn>1600-0641</eissn><coden>JOHEEC</coden><abstract>Background/Aim: Vertical transmission of hepatitis C virus (HCV) is well established but its incidence is low. To assess the molecular evidence of mother-to-infant transmission or intrafamilial transmission of HCV, the NS5 B region and the hypervariable region 1 (HVR1) of the E2/NS1 region of the HCV genome from each member of a family were investigated.
Methods: A 35-year-old mother with chronic hepatitis C virus infection and her four infected boys were studied. The same HCV 1a genotype was found in all five. Phylogenetic analysis was done by the neighbor-joining, the maximum likelihood, and the maximum parsimony methods.
Results: Comparison of the phylogenetic trees in the NS5B and HVR1 regions showed that the sequences in the children were more closely related to the population of variants of their own mother than to any genotype 1a sequence available in the databases. However, four HVR1 clones from two brothers (E2 and E3) had a strong homology, but were significantly divergent from the variants of the mother.
Conclusions: These results suggest that a cluster of HCV strains exists in the family and that E3 could have been superinfected by E2 HCV strains and reciprocally. In conclusion, phylogenetic analysis through variable regions of the genome suggests that at least two modes of transmission are involved in this family: perinatal and horizontal.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>10406172</pmid><doi>10.1016/S0168-8278(99)80248-7</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of hepatology, 1999-06, Vol.30 (6), p.970-978 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Biological and medical sciences Child Child, Preschool Disease Transmission, Infectious Female Hepatitis B virus - genetics Hepatitis C virus Hepatitis C, Chronic - etiology Hepatitis C, Chronic - virology Human viral diseases Humans Infectious Disease Transmission, Vertical Infectious diseases Intrafamilial transmission Male Medical sciences Mother-to-infant transmission Quasispecies RNA, Viral - analysis Sequence Homology Superinfection Viral diseases Viral hepatitis |
| title | Mother-to-infant transmission of hepatitis C virus: molecular evidence of superinfection by homologous virus in children |
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