Immunohistochemical localization of carbonic anhydrase isozyme II in the gustatory epithelium of the adult rat
The distribution of carbonic anhydrase isozyme II (CA II)-like immunoreactivity (-LI) in the gustatory epithelium was examined in the adult rat. In the circumvallate and foliate papillae, CA II-LI was observed in the cytoplasm of the spindle-shaped taste bud cells, with weak immunoreaction in the su...
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| Veröffentlicht in: | Chemical senses 1999-06, Vol.24 (3), p.255-261 |
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| creator | DAIKOKU, H MORISAKI, I OGAWA, Y MAEDA, T KURISU, K WAKISAKA, S |
| description | The distribution of carbonic anhydrase isozyme II (CA II)-like immunoreactivity (-LI) in the gustatory epithelium was examined in the adult rat. In the circumvallate and foliate papillae, CA II-LI was observed in the cytoplasm of the spindle-shaped taste bud cells, with weak immunoreaction in the surface of the gustatory epithelium. No neuronal elements displayed CA II-LI in these papillae. There was no apparent difference in the distribution pattern between the anterior and posterior portions of the foliate papillae. In immunoelectron microscopy, immunoreaction products for CA II were diffusely distributed in the entire cytoplasm of the taste bud cells having dense round granules at the periphery of the cells. No taste bud cells displaying CA II-LI were detected in the fungiform papillae, but a few thick nerve fibers displayed CA II-LI. In the taste buds of the palatal epithelium, neither taste bud cells nor neuronal elements exhibited CA II-LI. The present results indicate that CA II was localized in the type I cells designated as supporting cells in the taste buds located in the posterior lingual papillae of the adult animal. |
| doi_str_mv | 10.1093/chemse/24.3.255 |
| format | Article |
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In the circumvallate and foliate papillae, CA II-LI was observed in the cytoplasm of the spindle-shaped taste bud cells, with weak immunoreaction in the surface of the gustatory epithelium. No neuronal elements displayed CA II-LI in these papillae. There was no apparent difference in the distribution pattern between the anterior and posterior portions of the foliate papillae. In immunoelectron microscopy, immunoreaction products for CA II were diffusely distributed in the entire cytoplasm of the taste bud cells having dense round granules at the periphery of the cells. No taste bud cells displaying CA II-LI were detected in the fungiform papillae, but a few thick nerve fibers displayed CA II-LI. In the taste buds of the palatal epithelium, neither taste bud cells nor neuronal elements exhibited CA II-LI. The present results indicate that CA II was localized in the type I cells designated as supporting cells in the taste buds located in the posterior lingual papillae of the adult animal.</description><identifier>ISSN: 0379-864X</identifier><identifier>ISSN: 1464-3553</identifier><identifier>EISSN: 1464-3553</identifier><identifier>DOI: 10.1093/chemse/24.3.255</identifier><identifier>PMID: 10400443</identifier><identifier>CODEN: CHSED8</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Biological and medical sciences ; carbonic anhydrase ; carbonic anhydrase II ; Carbonic Anhydrases - analysis ; Carbonic Anhydrases - chemistry ; Epithelium - enzymology ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Isoenzymes - analysis ; Isoenzymes - chemistry ; lingual epithelium ; Male ; Olfactory system and olfaction. Gustatory system and gustation ; Palate - enzymology ; Palate, Soft - enzymology ; Rats ; Rats, Sprague-Dawley ; Taste Buds - enzymology ; Tongue - enzymology ; Vertebrates: nervous system and sense organs</subject><ispartof>Chemical senses, 1999-06, Vol.24 (3), p.255-261</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-a1ae6f820a78e262acb4a7415ffd796ebaeca6d46080a1f07571f59923e078113</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1792506$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10400443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DAIKOKU, H</creatorcontrib><creatorcontrib>MORISAKI, I</creatorcontrib><creatorcontrib>OGAWA, Y</creatorcontrib><creatorcontrib>MAEDA, T</creatorcontrib><creatorcontrib>KURISU, K</creatorcontrib><creatorcontrib>WAKISAKA, S</creatorcontrib><title>Immunohistochemical localization of carbonic anhydrase isozyme II in the gustatory epithelium of the adult rat</title><title>Chemical senses</title><addtitle>Chem Senses</addtitle><description>The distribution of carbonic anhydrase isozyme II (CA II)-like immunoreactivity (-LI) in the gustatory epithelium was examined in the adult rat. In the circumvallate and foliate papillae, CA II-LI was observed in the cytoplasm of the spindle-shaped taste bud cells, with weak immunoreaction in the surface of the gustatory epithelium. No neuronal elements displayed CA II-LI in these papillae. There was no apparent difference in the distribution pattern between the anterior and posterior portions of the foliate papillae. In immunoelectron microscopy, immunoreaction products for CA II were diffusely distributed in the entire cytoplasm of the taste bud cells having dense round granules at the periphery of the cells. No taste bud cells displaying CA II-LI were detected in the fungiform papillae, but a few thick nerve fibers displayed CA II-LI. In the taste buds of the palatal epithelium, neither taste bud cells nor neuronal elements exhibited CA II-LI. The present results indicate that CA II was localized in the type I cells designated as supporting cells in the taste buds located in the posterior lingual papillae of the adult animal.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>carbonic anhydrase</subject><subject>carbonic anhydrase II</subject><subject>Carbonic Anhydrases - analysis</subject><subject>Carbonic Anhydrases - chemistry</subject><subject>Epithelium - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Isoenzymes - analysis</subject><subject>Isoenzymes - chemistry</subject><subject>lingual epithelium</subject><subject>Male</subject><subject>Olfactory system and olfaction. Gustatory system and gustation</subject><subject>Palate - enzymology</subject><subject>Palate, Soft - enzymology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Taste Buds - enzymology</subject><subject>Tongue - enzymology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0379-864X</issn><issn>1464-3553</issn><issn>1464-3553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1rHDEQxUVwiM9OandGhUm3d_pafZTGOPGBIU0C6cScVsrJ7K7OkrY4__XZ5Q6cLs0MzPzeK95D6IaSNSWGb9zeD8VvmFjzNWvbD2hFhRQNb1t-gVaEK9NoKX5foqtSXgihgjP9CV1SIggRgq_QuB2GaUz7WGpazKKDHvdpnvENakwjTgE7yLs0Rodh3B-7DMXjWNLbcfB4u8VxxHXv8Z-pVKgpH7E_xPnQx2lYxMsPuqmvOEP9jD4G6Iv_ct7X6Ne3x58PT83zj-_bh_vnxglqagMUvAyaEVDaM8nA7QQoQdsQOmWk34F3IDshiSZAA1GtoqE1hnFPlKaUX6OvJ99DTq-TL9UOsTjf9zD6NBUrjdZCS_1fkCquiDALuDmBLqdSsg_2kOMA-WgpsUsX9tSFZcJyO3cxK27P1tNu8N0__Cn8Gbg7A1DmwEOG0cXyzinDWiL5X39plOs</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>DAIKOKU, H</creator><creator>MORISAKI, I</creator><creator>OGAWA, Y</creator><creator>MAEDA, T</creator><creator>KURISU, K</creator><creator>WAKISAKA, S</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Immunohistochemical localization of carbonic anhydrase isozyme II in the gustatory epithelium of the adult rat</title><author>DAIKOKU, H ; MORISAKI, I ; OGAWA, Y ; MAEDA, T ; KURISU, K ; WAKISAKA, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-a1ae6f820a78e262acb4a7415ffd796ebaeca6d46080a1f07571f59923e078113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>carbonic anhydrase</topic><topic>carbonic anhydrase II</topic><topic>Carbonic Anhydrases - analysis</topic><topic>Carbonic Anhydrases - chemistry</topic><topic>Epithelium - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Isoenzymes - analysis</topic><topic>Isoenzymes - chemistry</topic><topic>lingual epithelium</topic><topic>Male</topic><topic>Olfactory system and olfaction. Gustatory system and gustation</topic><topic>Palate - enzymology</topic><topic>Palate, Soft - enzymology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Taste Buds - enzymology</topic><topic>Tongue - enzymology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAIKOKU, H</creatorcontrib><creatorcontrib>MORISAKI, I</creatorcontrib><creatorcontrib>OGAWA, Y</creatorcontrib><creatorcontrib>MAEDA, T</creatorcontrib><creatorcontrib>KURISU, K</creatorcontrib><creatorcontrib>WAKISAKA, S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical senses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DAIKOKU, H</au><au>MORISAKI, I</au><au>OGAWA, Y</au><au>MAEDA, T</au><au>KURISU, K</au><au>WAKISAKA, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical localization of carbonic anhydrase isozyme II in the gustatory epithelium of the adult rat</atitle><jtitle>Chemical senses</jtitle><addtitle>Chem Senses</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>24</volume><issue>3</issue><spage>255</spage><epage>261</epage><pages>255-261</pages><issn>0379-864X</issn><issn>1464-3553</issn><eissn>1464-3553</eissn><coden>CHSED8</coden><abstract>The distribution of carbonic anhydrase isozyme II (CA II)-like immunoreactivity (-LI) in the gustatory epithelium was examined in the adult rat. In the circumvallate and foliate papillae, CA II-LI was observed in the cytoplasm of the spindle-shaped taste bud cells, with weak immunoreaction in the surface of the gustatory epithelium. No neuronal elements displayed CA II-LI in these papillae. There was no apparent difference in the distribution pattern between the anterior and posterior portions of the foliate papillae. In immunoelectron microscopy, immunoreaction products for CA II were diffusely distributed in the entire cytoplasm of the taste bud cells having dense round granules at the periphery of the cells. No taste bud cells displaying CA II-LI were detected in the fungiform papillae, but a few thick nerve fibers displayed CA II-LI. In the taste buds of the palatal epithelium, neither taste bud cells nor neuronal elements exhibited CA II-LI. The present results indicate that CA II was localized in the type I cells designated as supporting cells in the taste buds located in the posterior lingual papillae of the adult animal.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10400443</pmid><doi>10.1093/chemse/24.3.255</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological and medical sciences carbonic anhydrase carbonic anhydrase II Carbonic Anhydrases - analysis Carbonic Anhydrases - chemistry Epithelium - enzymology Fundamental and applied biological sciences. Psychology Immunohistochemistry Isoenzymes - analysis Isoenzymes - chemistry lingual epithelium Male Olfactory system and olfaction. Gustatory system and gustation Palate - enzymology Palate, Soft - enzymology Rats Rats, Sprague-Dawley Taste Buds - enzymology Tongue - enzymology Vertebrates: nervous system and sense organs |
| title | Immunohistochemical localization of carbonic anhydrase isozyme II in the gustatory epithelium of the adult rat |
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