Age-related changes of the nucleolar organizer regions without neuron loss in medial mamillary bodies (hypothalamus) in old rats

Age-related morphological and functional changes were studied in the medial mamillary nuclei of the hypothalamus (MMN). The number of nerve cells and the volume of MMN were estimated in both groups of Wistar rats, adult and old (3 and 22 months, respectively) using stereologic methods such as the op...

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Veröffentlicht in:	Mechanisms of ageing and development 1999-05, Vol.108 (2), p.113-125
Hauptverfasser:	Begega, A, Cuesta, M, Rubio, S, Santı́n, L.J, Arias, J.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Ag-NORs Aging - physiology Animals Cavalieri principle Hypothalamus - physiology Male Mamillary bodies Mammillary Bodies - physiology Neurons - physiology Nucleolus Organizer Region - physiology Optical fractionator Rats Rats, Wistar
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container_title	Mechanisms of ageing and development
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creator	Begega, A Cuesta, M Rubio, S Santı́n, L.J Arias, J.L
description	Age-related morphological and functional changes were studied in the medial mamillary nuclei of the hypothalamus (MMN). The number of nerve cells and the volume of MMN were estimated in both groups of Wistar rats, adult and old (3 and 22 months, respectively) using stereologic methods such as the optical fractionator and Cavalieri’s method respectively. The number of neurons and glial cells remain inalterable with ageing but there was an age-dependent reduction in MMN volume. In the second experiment, functional changes in the MMN neurons were observed although there was no loss in neuron number. Several functional parameters of the Ag-NORs were quantified by a computer analysis system: area and number of Ag-NORs per neuron; number of neurons with one or several Ag-NORs and also surface of neuronal nucleus. The present study shows how ageing causes volumetric and functional changes in the MMN whereas the number of neurons and glial cells remain unchanged in the mamillary region. All these results confirm the effects of age on proteic synthesis activity in neurons of the MMN, showing a decreased neuronal activity in this region.
doi_str_mv	10.1016/S0047-6374(98)00157-2
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subjects	Ag-NORs Aging - physiology Animals Cavalieri principle Hypothalamus - physiology Male Mamillary bodies Mammillary Bodies - physiology Neurons - physiology Nucleolus Organizer Region - physiology Optical fractionator Rats Rats, Wistar
title	Age-related changes of the nucleolar organizer regions without neuron loss in medial mamillary bodies (hypothalamus) in old rats
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