Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp
Narp (neuronal activity-regulated pentraxin) is a secreted immediate-early gene (IEG) regulated by synaptic activity in brain. In this study, we demonstrate that Narp possesses several properties that make it likely to play a key role in excitatory synaptogenesis. Narp is shown to be selectively enr...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1999-06, Vol.23 (2), p.309-323 |
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| creator | O'Brien, R J Xu, D Petralia, R S Steward, O Huganir, R L Worley, P |
| description | Narp (neuronal activity-regulated pentraxin) is a secreted immediate-early gene (IEG) regulated by synaptic activity in brain. In this study, we demonstrate that Narp possesses several properties that make it likely to play a key role in excitatory synaptogenesis. Narp is shown to be selectively enriched at excitatory synapses on neurons from both the hippocampus and spinal cord. Overexpression of recombinant Narp increases the number of excitatory but not inhibitory synapses in cultured spinal neurons. In transfected HEK 293T cells, Narp interacts with itself, forming large surface clusters that coaggregate AMPA receptor subunits. Moreover, Narp-expressing HEK 293T cells can induce the aggregation of neuronal AMPA receptors. These studies support a model in which Narp functions as an extracellular aggregating factor for AMPA receptors. |
| doi_str_mv | 10.1016/s0896-6273(00)80782-5 |
| format | Article |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Axons - metabolism Blotting, Western C-Reactive Protein - biosynthesis C-Reactive Protein - physiology Cell Line Cells, Cultured Dendrites - metabolism Extracellular Space - metabolism Extracellular Space - physiology Hippocampus - cytology Immediate-Early Proteins - biosynthesis Immediate-Early Proteins - physiology Immunohistochemistry Microscopy, Electron Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - physiology Neurons - cytology Neurons - metabolism Rats Rats, Sprague-Dawley Receptors, AMPA - biosynthesis Receptors, AMPA - metabolism Receptors, AMPA - physiology Spinal Cord - cytology Synapses - metabolism Synapses - physiology Transfection |
| title | Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp |
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