Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: Preparation, characterization and delivery of a model protein
Purpose: To prepare and characterize polyethylenglycol-co-poly-D,L-lactide (PEG-D,L-PLA) multiblock copolymer microspheres containing ovalbumin. Microsphere batches made of Poly-D,L-lactide (PLA) homopolymers were prepared in order to evaluate how the presence of PEG segments into PEG-D,L-PLA copoly...
Gespeichert in:
| Veröffentlicht in: | Journal of microencapsulation 2008-01, Vol.25 (5), p.330-338 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 338 |
|---|---|
| container_issue | 5 |
| container_start_page | 330 |
| container_title | Journal of microencapsulation |
| container_volume | 25 |
| creator | Dorati, R. Genta, I. Tomasi, C. Modena, T. Colonna, C. Pavanetto, F. Perugini, P. Conti, B. |
| description | Purpose: To prepare and characterize polyethylenglycol-co-poly-D,L-lactide (PEG-D,L-PLA) multiblock copolymer microspheres containing ovalbumin. Microsphere batches made of Poly-D,L-lactide (PLA) homopolymers were prepared in order to evaluate how the presence of PEG segments into PEG-D,L-PLA copolymer could affect the behaviour of microspheres as carrier of protein drugs.
Methods: The PEG-D,L-PLA and PLA microspheres, loaded with the model protein ovalbumin, were prepared using double emulsion solvent evaporation method. The effect of PEG segments in the microparticles matrix, on the morphology, size distribution, encapsulation efficiency and release behaviour was studied.
Results: According to the results, PEG-D,L-PLA microspheres were more hydrophilic than PLA microparticles and with lower glass transition temperature. The surface of PEG-D,L-PLA microspheres was not as smooth as that of PLA microparticles, the mean diameter of PEG-D,L-PLA microparticles was bigger than that of PLA microspheres. Protein release from the microspheres was affected by the morphological structure of PEG-D,L-PLA microspheres and properties of PEG-D,L-PLA copolymer. This study suggests that PEG-D,L-PLA multiblock copolymer may be used as carrier in protein delivery systems for different purposes. |
| doi_str_mv | 10.1080/02652040801996763 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_69873616</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69873616</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-55587be8b353c4df34b8d93330b1e8023cf87f7703ded2e1a5a317e32bfdbb0c3</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhS0EotPCD2CDvIFVA37EjwAbVJ7SSHQB68ixb4grJw52BpTu-Od4mAGEkMrK18ffub72QegBJU8o0eQpYVIwUpeSNo1Ukt9CG1rLuhKslrfRZn9eFUCfoNOcrwghotHsLjqhupaCE7FB3y9jWGEZ1gDT57DaGCobq7mI1avzbRWMXbwDbONeGiHhzmRwePQ2xTwPkCA_w5cJZpPM4uN0ju1QSrtA8tc_FWwmhx0E_xXSimOPDR5j2eM5xQX8dA_d6U3IcP-4nqFPb15_vHhXbT-8fX_xclvZMutSCSG06kB3XHBbu57XnXYN55x0FDRh3PZa9UoR7sAxoEYYThVw1vWu64jlZ-jxoW-598sO8tKOPlsIwUwQd7mVjVZcUvlfkBEiVaN1AekB3P9FTtC3c_KjSWtLSbsPqP0noOJ5eGy-60ZwfxzHRArw6AiYbE3ok5msz785RoRQTa0K9-LA-amPaTTfYgquXcwaYvpl4jfN8fwv-wAmLIM1CdqruEtTSeKGV_wAA8S_6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20067988</pqid></control><display><type>article</type><title>Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: Preparation, characterization and delivery of a model protein</title><source>Taylor & Francis</source><source>MEDLINE</source><source>Taylor & Francis Medical Library - CRKN</source><creator>Dorati, R. ; Genta, I. ; Tomasi, C. ; Modena, T. ; Colonna, C. ; Pavanetto, F. ; Perugini, P. ; Conti, B.</creator><creatorcontrib>Dorati, R. ; Genta, I. ; Tomasi, C. ; Modena, T. ; Colonna, C. ; Pavanetto, F. ; Perugini, P. ; Conti, B.</creatorcontrib><description>Purpose: To prepare and characterize polyethylenglycol-co-poly-D,L-lactide (PEG-D,L-PLA) multiblock copolymer microspheres containing ovalbumin. Microsphere batches made of Poly-D,L-lactide (PLA) homopolymers were prepared in order to evaluate how the presence of PEG segments into PEG-D,L-PLA copolymer could affect the behaviour of microspheres as carrier of protein drugs.
Methods: The PEG-D,L-PLA and PLA microspheres, loaded with the model protein ovalbumin, were prepared using double emulsion solvent evaporation method. The effect of PEG segments in the microparticles matrix, on the morphology, size distribution, encapsulation efficiency and release behaviour was studied.
Results: According to the results, PEG-D,L-PLA microspheres were more hydrophilic than PLA microparticles and with lower glass transition temperature. The surface of PEG-D,L-PLA microspheres was not as smooth as that of PLA microparticles, the mean diameter of PEG-D,L-PLA microparticles was bigger than that of PLA microspheres. Protein release from the microspheres was affected by the morphological structure of PEG-D,L-PLA microspheres and properties of PEG-D,L-PLA copolymer. This study suggests that PEG-D,L-PLA multiblock copolymer may be used as carrier in protein delivery systems for different purposes.</description><identifier>ISSN: 0265-2048</identifier><identifier>EISSN: 1464-5246</identifier><identifier>DOI: 10.1080/02652040801996763</identifier><identifier>PMID: 18465305</identifier><identifier>CODEN: JOMIEF</identifier><language>eng</language><publisher>Colchester: Informa UK Ltd</publisher><subject>Biological and medical sciences ; Chemistry, Pharmaceutical - methods ; Drug Carriers ; Drug Compounding - methods ; Drug Delivery Systems ; Drug Design ; General pharmacology ; Hydrogen-Ion Concentration ; Lactates - chemistry ; Medical sciences ; Microscopy, Electron, Scanning ; Microspheres ; Molecular Weight ; multiblock copolymers ; ovalbumin ; Ovalbumin - chemistry ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Polyethylene Glycols - chemistry ; polyethylenglycol ; polylactide ; Polymers - chemistry ; Sodium Chloride - chemistry ; Temperature</subject><ispartof>Journal of microencapsulation, 2008-01, Vol.25 (5), p.330-338</ispartof><rights>2008 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-55587be8b353c4df34b8d93330b1e8023cf87f7703ded2e1a5a317e32bfdbb0c3</citedby><cites>FETCH-LOGICAL-c465t-55587be8b353c4df34b8d93330b1e8023cf87f7703ded2e1a5a317e32bfdbb0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/02652040801996763$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/02652040801996763$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20557947$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18465305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dorati, R.</creatorcontrib><creatorcontrib>Genta, I.</creatorcontrib><creatorcontrib>Tomasi, C.</creatorcontrib><creatorcontrib>Modena, T.</creatorcontrib><creatorcontrib>Colonna, C.</creatorcontrib><creatorcontrib>Pavanetto, F.</creatorcontrib><creatorcontrib>Perugini, P.</creatorcontrib><creatorcontrib>Conti, B.</creatorcontrib><title>Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: Preparation, characterization and delivery of a model protein</title><title>Journal of microencapsulation</title><addtitle>J Microencapsul</addtitle><description>Purpose: To prepare and characterize polyethylenglycol-co-poly-D,L-lactide (PEG-D,L-PLA) multiblock copolymer microspheres containing ovalbumin. Microsphere batches made of Poly-D,L-lactide (PLA) homopolymers were prepared in order to evaluate how the presence of PEG segments into PEG-D,L-PLA copolymer could affect the behaviour of microspheres as carrier of protein drugs.
Methods: The PEG-D,L-PLA and PLA microspheres, loaded with the model protein ovalbumin, were prepared using double emulsion solvent evaporation method. The effect of PEG segments in the microparticles matrix, on the morphology, size distribution, encapsulation efficiency and release behaviour was studied.
Results: According to the results, PEG-D,L-PLA microspheres were more hydrophilic than PLA microparticles and with lower glass transition temperature. The surface of PEG-D,L-PLA microspheres was not as smooth as that of PLA microparticles, the mean diameter of PEG-D,L-PLA microparticles was bigger than that of PLA microspheres. Protein release from the microspheres was affected by the morphological structure of PEG-D,L-PLA microspheres and properties of PEG-D,L-PLA copolymer. This study suggests that PEG-D,L-PLA multiblock copolymer may be used as carrier in protein delivery systems for different purposes.</description><subject>Biological and medical sciences</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>Drug Carriers</subject><subject>Drug Compounding - methods</subject><subject>Drug Delivery Systems</subject><subject>Drug Design</subject><subject>General pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lactates - chemistry</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microspheres</subject><subject>Molecular Weight</subject><subject>multiblock copolymers</subject><subject>ovalbumin</subject><subject>Ovalbumin - chemistry</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyethylene Glycols - chemistry</subject><subject>polyethylenglycol</subject><subject>polylactide</subject><subject>Polymers - chemistry</subject><subject>Sodium Chloride - chemistry</subject><subject>Temperature</subject><issn>0265-2048</issn><issn>1464-5246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EotPCD2CDvIFVA37EjwAbVJ7SSHQB68ixb4grJw52BpTu-Od4mAGEkMrK18ffub72QegBJU8o0eQpYVIwUpeSNo1Ukt9CG1rLuhKslrfRZn9eFUCfoNOcrwghotHsLjqhupaCE7FB3y9jWGEZ1gDT57DaGCobq7mI1avzbRWMXbwDbONeGiHhzmRwePQ2xTwPkCA_w5cJZpPM4uN0ju1QSrtA8tc_FWwmhx0E_xXSimOPDR5j2eM5xQX8dA_d6U3IcP-4nqFPb15_vHhXbT-8fX_xclvZMutSCSG06kB3XHBbu57XnXYN55x0FDRh3PZa9UoR7sAxoEYYThVw1vWu64jlZ-jxoW-598sO8tKOPlsIwUwQd7mVjVZcUvlfkBEiVaN1AekB3P9FTtC3c_KjSWtLSbsPqP0noOJ5eGy-60ZwfxzHRArw6AiYbE3ok5msz785RoRQTa0K9-LA-amPaTTfYgquXcwaYvpl4jfN8fwv-wAmLIM1CdqruEtTSeKGV_wAA8S_6g</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Dorati, R.</creator><creator>Genta, I.</creator><creator>Tomasi, C.</creator><creator>Modena, T.</creator><creator>Colonna, C.</creator><creator>Pavanetto, F.</creator><creator>Perugini, P.</creator><creator>Conti, B.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Informa</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: Preparation, characterization and delivery of a model protein</title><author>Dorati, R. ; Genta, I. ; Tomasi, C. ; Modena, T. ; Colonna, C. ; Pavanetto, F. ; Perugini, P. ; Conti, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-55587be8b353c4df34b8d93330b1e8023cf87f7703ded2e1a5a317e32bfdbb0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>Drug Carriers</topic><topic>Drug Compounding - methods</topic><topic>Drug Delivery Systems</topic><topic>Drug Design</topic><topic>General pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lactates - chemistry</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microspheres</topic><topic>Molecular Weight</topic><topic>multiblock copolymers</topic><topic>ovalbumin</topic><topic>Ovalbumin - chemistry</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyethylene Glycols - chemistry</topic><topic>polyethylenglycol</topic><topic>polylactide</topic><topic>Polymers - chemistry</topic><topic>Sodium Chloride - chemistry</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dorati, R.</creatorcontrib><creatorcontrib>Genta, I.</creatorcontrib><creatorcontrib>Tomasi, C.</creatorcontrib><creatorcontrib>Modena, T.</creatorcontrib><creatorcontrib>Colonna, C.</creatorcontrib><creatorcontrib>Pavanetto, F.</creatorcontrib><creatorcontrib>Perugini, P.</creatorcontrib><creatorcontrib>Conti, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microencapsulation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dorati, R.</au><au>Genta, I.</au><au>Tomasi, C.</au><au>Modena, T.</au><au>Colonna, C.</au><au>Pavanetto, F.</au><au>Perugini, P.</au><au>Conti, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: Preparation, characterization and delivery of a model protein</atitle><jtitle>Journal of microencapsulation</jtitle><addtitle>J Microencapsul</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>25</volume><issue>5</issue><spage>330</spage><epage>338</epage><pages>330-338</pages><issn>0265-2048</issn><eissn>1464-5246</eissn><coden>JOMIEF</coden><abstract>Purpose: To prepare and characterize polyethylenglycol-co-poly-D,L-lactide (PEG-D,L-PLA) multiblock copolymer microspheres containing ovalbumin. Microsphere batches made of Poly-D,L-lactide (PLA) homopolymers were prepared in order to evaluate how the presence of PEG segments into PEG-D,L-PLA copolymer could affect the behaviour of microspheres as carrier of protein drugs.
Methods: The PEG-D,L-PLA and PLA microspheres, loaded with the model protein ovalbumin, were prepared using double emulsion solvent evaporation method. The effect of PEG segments in the microparticles matrix, on the morphology, size distribution, encapsulation efficiency and release behaviour was studied.
Results: According to the results, PEG-D,L-PLA microspheres were more hydrophilic than PLA microparticles and with lower glass transition temperature. The surface of PEG-D,L-PLA microspheres was not as smooth as that of PLA microparticles, the mean diameter of PEG-D,L-PLA microparticles was bigger than that of PLA microspheres. Protein release from the microspheres was affected by the morphological structure of PEG-D,L-PLA microspheres and properties of PEG-D,L-PLA copolymer. This study suggests that PEG-D,L-PLA multiblock copolymer may be used as carrier in protein delivery systems for different purposes.</abstract><cop>Colchester</cop><pub>Informa UK Ltd</pub><pmid>18465305</pmid><doi>10.1080/02652040801996763</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0265-2048 |
| ispartof | Journal of microencapsulation, 2008-01, Vol.25 (5), p.330-338 |
| issn | 0265-2048 1464-5246 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69873616 |
| source | Taylor & Francis; MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Biological and medical sciences Chemistry, Pharmaceutical - methods Drug Carriers Drug Compounding - methods Drug Delivery Systems Drug Design General pharmacology Hydrogen-Ion Concentration Lactates - chemistry Medical sciences Microscopy, Electron, Scanning Microspheres Molecular Weight multiblock copolymers ovalbumin Ovalbumin - chemistry Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Polyethylene Glycols - chemistry polyethylenglycol polylactide Polymers - chemistry Sodium Chloride - chemistry Temperature |
| title | Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: Preparation, characterization and delivery of a model protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T07%3A32%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Polyethylenglycol-co-poly-D,L-lactide%20copolymer%20based%20microspheres:%20Preparation,%20characterization%20and%20delivery%20of%20a%20model%20protein&rft.jtitle=Journal%20of%20microencapsulation&rft.au=Dorati,%20R.&rft.date=2008-01-01&rft.volume=25&rft.issue=5&rft.spage=330&rft.epage=338&rft.pages=330-338&rft.issn=0265-2048&rft.eissn=1464-5246&rft.coden=JOMIEF&rft_id=info:doi/10.1080/02652040801996763&rft_dat=%3Cproquest_pasca%3E69873616%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20067988&rft_id=info:pmid/18465305&rfr_iscdi=true |