Gene transfer and living release of transforming growth factor-beta3 for cartilage tissue engineering applications
Cartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors...
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| Veröffentlicht in: | Tissue engineering. Part C, Methods Methods, 2008-12, Vol.14 (4), p.273-280 |
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| container_title | Tissue engineering. Part C, Methods |
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| creator | Hao, Jinghua Yao, Yongchang Varshney, Rohan R Wang, Laicheng Prakash, Celine Li, Hao Wang, Dong-An |
| description | Cartilage restoration continues to present a tremendous clinical challenge due to its nonvascular nature. Many studies have demonstrated that chondrogenesis of progenitor cells can be achieved in vitro by manual dose of growth factors; however, it remains a vital difficulty in feeding growth factors to implanted therapeutic cells in vivo. Herein, we constructed recombinant adenovirus encoding human transforming growth factor-beta3 (hTGF-beta3) and practiced it in rat bone marrow-derived mesenchymal stromal cells and articular chondrocytes for cartilage regeneration. Optimal viable transduction and transgenic hTGF-beta3 production were achieved; consequently, positive expression of cartilage marker-collagen type II was enabled in the infected progenitors. We thus conclude that recombinant adenovirus encoding TGF-beta3 gene has been successfully established and validated for cartilage tissue engineering applications. |
| doi_str_mv | 10.1089/ten.tec.2008.0163 |
| format | Article |
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| ispartof | Tissue engineering. Part C, Methods, 2008-12, Vol.14 (4), p.273-280 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Bone Marrow Cells - cytology Cartilage - cytology Cartilage - metabolism Cell Differentiation Cell Line Gene Transfer Techniques Humans Male Models, Biological Rats Rats, Wistar Stromal Cells - cytology Tissue Engineering - methods Transforming Growth Factor beta3 - metabolism Transgenes |
| title | Gene transfer and living release of transforming growth factor-beta3 for cartilage tissue engineering applications |
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