A Distinctive RNA Fold: The Solution Structure of an Analogue of the Yeast tRNAPhe TΨC Domain
The structure of an analogue of the yeast tRNAPhe TΨC stem−loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified TΨC stem−loop is the produ...
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Veröffentlicht in: | Biochemistry (Easton) 1999-07, Vol.38 (27), p.8647-8656 |
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creator | Koshlap, Karl M Guenther, Richard Sochacka, Elzbieta Malkiewicz, Andrzej Agris, Paul F |
description | The structure of an analogue of the yeast tRNAPhe TΨC stem−loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified TΨC stem−loop is the product of the m5U54-tRNA methyltransferase, but is not a substrate for the m1A58-tRNA methyltransferase. Site-specific substitutions and 15N labels were used to confirm the assignment of NOESY cross-peaks critical in defining the global fold of the molecule. The structure is unusual in that the loop folds far over into the major groove of the curved stem. This conformation is stabilized by both stacking interactions and hydrogen bond formation. Furthermore, this conformation appears to be unique among RNA hairpins of similar size. There is, however, a considerable resemblance to the analogous domain in the crystal structure of the full-length yeast tRNAPhe. We believe, therefore, that the structure we have determined may represent an intermediate in the folding pathway during the maturation of tRNA. |
doi_str_mv | 10.1021/bi990118w |
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The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified TΨC stem−loop is the product of the m5U54-tRNA methyltransferase, but is not a substrate for the m1A58-tRNA methyltransferase. Site-specific substitutions and 15N labels were used to confirm the assignment of NOESY cross-peaks critical in defining the global fold of the molecule. The structure is unusual in that the loop folds far over into the major groove of the curved stem. This conformation is stabilized by both stacking interactions and hydrogen bond formation. Furthermore, this conformation appears to be unique among RNA hairpins of similar size. There is, however, a considerable resemblance to the analogous domain in the crystal structure of the full-length yeast tRNAPhe. We believe, therefore, that the structure we have determined may represent an intermediate in the folding pathway during the maturation of tRNA.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi990118w</identifier><identifier>PMID: 10393540</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Anticodon - chemistry ; Base Sequence ; Crystallography, X-Ray ; Models, Molecular ; Molecular Sequence Data ; Nuclear Magnetic Resonance, Biomolecular - methods ; Nucleic Acid Conformation ; Pseudouridine - chemistry ; RNA, Fungal - chemistry ; RNA, Transfer, Phe - chemistry ; Saccharomyces cerevisiae - chemistry ; Solutions ; Uridine - analogs & derivatives ; Uridine - chemistry</subject><ispartof>Biochemistry (Easton), 1999-07, Vol.38 (27), p.8647-8656</ispartof><rights>Copyright © 1999 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi990118w$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi990118w$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10393540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koshlap, Karl M</creatorcontrib><creatorcontrib>Guenther, Richard</creatorcontrib><creatorcontrib>Sochacka, Elzbieta</creatorcontrib><creatorcontrib>Malkiewicz, Andrzej</creatorcontrib><creatorcontrib>Agris, Paul F</creatorcontrib><title>A Distinctive RNA Fold: The Solution Structure of an Analogue of the Yeast tRNAPhe TΨC Domain</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>The structure of an analogue of the yeast tRNAPhe TΨC stem−loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified TΨC stem−loop is the product of the m5U54-tRNA methyltransferase, but is not a substrate for the m1A58-tRNA methyltransferase. Site-specific substitutions and 15N labels were used to confirm the assignment of NOESY cross-peaks critical in defining the global fold of the molecule. The structure is unusual in that the loop folds far over into the major groove of the curved stem. This conformation is stabilized by both stacking interactions and hydrogen bond formation. Furthermore, this conformation appears to be unique among RNA hairpins of similar size. There is, however, a considerable resemblance to the analogous domain in the crystal structure of the full-length yeast tRNAPhe. We believe, therefore, that the structure we have determined may represent an intermediate in the folding pathway during the maturation of tRNA.</description><subject>Anticodon - chemistry</subject><subject>Base Sequence</subject><subject>Crystallography, X-Ray</subject><subject>Models, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Magnetic Resonance, Biomolecular - methods</subject><subject>Nucleic Acid Conformation</subject><subject>Pseudouridine - chemistry</subject><subject>RNA, Fungal - chemistry</subject><subject>RNA, Transfer, Phe - chemistry</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Solutions</subject><subject>Uridine - analogs & derivatives</subject><subject>Uridine - chemistry</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1OwzAUhC0EoqWw4ALIG9gFbMdxYnZVSwtSVUpbBKwsJ3EgkMYldvjZseUmnIZDcBJcWrp6Gr1Po9EMAPsYHWNE8Emcc44wjl43QBMHBHmU82ATNBFCzCOcoQbYMebRSYpCug0aGPncDyhqAtmG3dzYvExs_qLgeNiGPV2kpz8fn3D6oOBEF7XNdQkntqoTW1cK6gzKErZLWej7-k9aB94paSy0zmDk1PT7qwO7eibzchdsZbIwam91W-C6dzbtnHuDy_5Fpz3wJCaLyCHDzKdZzBTHscpS6mPG4jTkKY59khCSoUgpHEUBY1imiGYBlVEShgTFmHK_BY6WvvNKP9fKWDHLTaKKQpZK10YwHrGAE-TAgxVYxzOVinmVz2T1Lv5LcYC3BFwx6m39l9WTYKEfBmI6mojBTciv-sOxuHX84ZKXiRGPuq5cNcbZicU4Yj2O_wuciXzj</recordid><startdate>19990706</startdate><enddate>19990706</enddate><creator>Koshlap, Karl M</creator><creator>Guenther, Richard</creator><creator>Sochacka, Elzbieta</creator><creator>Malkiewicz, Andrzej</creator><creator>Agris, Paul F</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19990706</creationdate><title>A Distinctive RNA Fold: The Solution Structure of an Analogue of the Yeast tRNAPhe TΨC Domain</title><author>Koshlap, Karl M ; Guenther, Richard ; Sochacka, Elzbieta ; Malkiewicz, Andrzej ; Agris, Paul F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a1220-4761634fb6e91befd43166bd79d1b32c22f08ee1885661ad04f54a8c7720b1493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Anticodon - chemistry</topic><topic>Base Sequence</topic><topic>Crystallography, X-Ray</topic><topic>Models, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Magnetic Resonance, Biomolecular - methods</topic><topic>Nucleic Acid Conformation</topic><topic>Pseudouridine - chemistry</topic><topic>RNA, Fungal - chemistry</topic><topic>RNA, Transfer, Phe - chemistry</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Solutions</topic><topic>Uridine - analogs & derivatives</topic><topic>Uridine - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koshlap, Karl M</creatorcontrib><creatorcontrib>Guenther, Richard</creatorcontrib><creatorcontrib>Sochacka, Elzbieta</creatorcontrib><creatorcontrib>Malkiewicz, Andrzej</creatorcontrib><creatorcontrib>Agris, Paul F</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koshlap, Karl M</au><au>Guenther, Richard</au><au>Sochacka, Elzbieta</au><au>Malkiewicz, Andrzej</au><au>Agris, Paul F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Distinctive RNA Fold: The Solution Structure of an Analogue of the Yeast tRNAPhe TΨC Domain</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1999-07-06</date><risdate>1999</risdate><volume>38</volume><issue>27</issue><spage>8647</spage><epage>8656</epage><pages>8647-8656</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>The structure of an analogue of the yeast tRNAPhe TΨC stem−loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified TΨC stem−loop is the product of the m5U54-tRNA methyltransferase, but is not a substrate for the m1A58-tRNA methyltransferase. Site-specific substitutions and 15N labels were used to confirm the assignment of NOESY cross-peaks critical in defining the global fold of the molecule. The structure is unusual in that the loop folds far over into the major groove of the curved stem. This conformation is stabilized by both stacking interactions and hydrogen bond formation. Furthermore, this conformation appears to be unique among RNA hairpins of similar size. There is, however, a considerable resemblance to the analogous domain in the crystal structure of the full-length yeast tRNAPhe. We believe, therefore, that the structure we have determined may represent an intermediate in the folding pathway during the maturation of tRNA.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>10393540</pmid><doi>10.1021/bi990118w</doi><tpages>10</tpages></addata></record> |
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subjects | Anticodon - chemistry Base Sequence Crystallography, X-Ray Models, Molecular Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular - methods Nucleic Acid Conformation Pseudouridine - chemistry RNA, Fungal - chemistry RNA, Transfer, Phe - chemistry Saccharomyces cerevisiae - chemistry Solutions Uridine - analogs & derivatives Uridine - chemistry |
title | A Distinctive RNA Fold: The Solution Structure of an Analogue of the Yeast tRNAPhe TΨC Domain |
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