Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells
Abstract MSCs are known to have an extensive proliferative potential and ability to differentiate in various cell types. Osteoblastic differentiation from mesenchymal progenitor cells is an important step of bone formation, though the pattern of gene expression during differentiation is not yet well...
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| Veröffentlicht in: | Bone (New York, N.Y.) N.Y.), 2008-12, Vol.43 (6), p.1084-1092 |
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| creator | Valenti, Maria Teresa Carbonare, Luca Dalle Donatelli, Luca Bertoldo, Francesco Zanatta, Mirko Lo Cascio, Vincenzo |
| description | Abstract MSCs are known to have an extensive proliferative potential and ability to differentiate in various cell types. Osteoblastic differentiation from mesenchymal progenitor cells is an important step of bone formation, though the pattern of gene expression during differentiation is not yet well understood. Here, to investigate the possibility to obtain a model for in vitro bone differentiation using mesenchymal stem cells (hMSCs) from human subjects non-invasively, we developed a method to obtain hMSCs-like cells from peripheral blood by a two step method that included an enrichment of mononuclear cells followed by depletion of unwanted cells. Using these cells, we analyzed the expression of transcription factor genes (runt-related transcription factor 2 ( RUNX2 ) and osterix ( SP7 )) and bone related genes (osteopontin ( SPP1 ), osteonectin ( SPARC ) and collagen, type I, alpha 1 ( COLIA1 )) during osteoblastic differentiation. Our results demonstrated that hMSCs can be obtained from peripheral blood and that they are able to generate CFU-F and to differentiate in osteoblast and adipocyte; in this study, we also identified a possible gene expression timing during osteoblastic differentiation that provided a powerful tool to study bone physiology. |
| doi_str_mv | 10.1016/j.bone.2008.07.252 |
| format | Article |
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Osteoblastic differentiation from mesenchymal progenitor cells is an important step of bone formation, though the pattern of gene expression during differentiation is not yet well understood. Here, to investigate the possibility to obtain a model for in vitro bone differentiation using mesenchymal stem cells (hMSCs) from human subjects non-invasively, we developed a method to obtain hMSCs-like cells from peripheral blood by a two step method that included an enrichment of mononuclear cells followed by depletion of unwanted cells. Using these cells, we analyzed the expression of transcription factor genes (runt-related transcription factor 2 ( RUNX2 ) and osterix ( SP7 )) and bone related genes (osteopontin ( SPP1 ), osteonectin ( SPARC ) and collagen, type I, alpha 1 ( COLIA1 )) during osteoblastic differentiation. Our results demonstrated that hMSCs can be obtained from peripheral blood and that they are able to generate CFU-F and to differentiate in osteoblast and adipocyte; in this study, we also identified a possible gene expression timing during osteoblastic differentiation that provided a powerful tool to study bone physiology.</description><identifier>ISSN: 8756-3282</identifier><identifier>EISSN: 1873-2763</identifier><identifier>DOI: 10.1016/j.bone.2008.07.252</identifier><identifier>PMID: 18761114</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Differentiation ; Cells, Cultured ; CFU-F ; Differentiation ; Diseases of the osteoarticular system ; Fundamental and applied biological sciences. Psychology ; Gene expression analysis ; Gene Expression Profiling ; Humans ; Immunohistochemistry ; Medical sciences ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Orthopedics ; Osteoblast ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription Factors - genetics ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Bone (New York, N.Y.), 2008-12, Vol.43 (6), p.1084-1092</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-36dee14d236677788c76dcc6c53ea9cfb5b1e6c3595a72d011dee414e80990a33</citedby><cites>FETCH-LOGICAL-c536t-36dee14d236677788c76dcc6c53ea9cfb5b1e6c3595a72d011dee414e80990a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bone.2008.07.252$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20971975$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18761114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valenti, Maria Teresa</creatorcontrib><creatorcontrib>Carbonare, Luca Dalle</creatorcontrib><creatorcontrib>Donatelli, Luca</creatorcontrib><creatorcontrib>Bertoldo, Francesco</creatorcontrib><creatorcontrib>Zanatta, Mirko</creatorcontrib><creatorcontrib>Lo Cascio, Vincenzo</creatorcontrib><title>Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells</title><title>Bone (New York, N.Y.)</title><addtitle>Bone</addtitle><description>Abstract MSCs are known to have an extensive proliferative potential and ability to differentiate in various cell types. Osteoblastic differentiation from mesenchymal progenitor cells is an important step of bone formation, though the pattern of gene expression during differentiation is not yet well understood. Here, to investigate the possibility to obtain a model for in vitro bone differentiation using mesenchymal stem cells (hMSCs) from human subjects non-invasively, we developed a method to obtain hMSCs-like cells from peripheral blood by a two step method that included an enrichment of mononuclear cells followed by depletion of unwanted cells. Using these cells, we analyzed the expression of transcription factor genes (runt-related transcription factor 2 ( RUNX2 ) and osterix ( SP7 )) and bone related genes (osteopontin ( SPP1 ), osteonectin ( SPARC ) and collagen, type I, alpha 1 ( COLIA1 )) during osteoblastic differentiation. Our results demonstrated that hMSCs can be obtained from peripheral blood and that they are able to generate CFU-F and to differentiate in osteoblast and adipocyte; in this study, we also identified a possible gene expression timing during osteoblastic differentiation that provided a powerful tool to study bone physiology.</description><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>CFU-F</subject><subject>Differentiation</subject><subject>Diseases of the osteoarticular system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression analysis</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Orthopedics</subject><subject>Osteoblast</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transcription Factors - genetics</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>8756-3282</issn><issn>1873-2763</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkGLFDEQhYMo7uzqH_AgfdFbt5VkknRAhGXRVVjwoJ5DOl3NZuzujKkecf69aWZYwYOeAsn3qirvFWMvODQcuH6za7o0YyMA2gZMI5R4xDa8NbIWRsvHbNMapWspWnHBLol2ACCt4U_ZRYE053y7YXiLM1b4a5-RKKa58rMfjxSpinOVaMHUjZ6WGKo-DgNmnJfolxUccpqqPea4v8fsx6obU-qrCQnncH-cyk1RT1XAcaRn7MngR8Ln5_OKffvw_uvNx_ru8-2nm-u7Oiipl1rqHpFveyG1Nsa0bTC6D0GXV_Q2DJ3qOOoglVXeiB44L_yWb7EFa8FLecVen-ruc_pxQFrcFGmdwM-YDuS0bbWSFv4Lcmus0lYVUJzAkBNRxsHtc5x8PjoObk3B7dyagltTcGBcSaGIXp6rH7oJ-z-Ss-0FeHUGPAU_DtnPIdIDJ6DEZM3a_e2Jw2Laz4jZUYjFX-xjxrC4PsV_z_HuL3kY4xxLx-94RNqlQy5pl986Eg7cl3Vf1nWBFkADKPkbNY-8VA</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Valenti, Maria Teresa</creator><creator>Carbonare, Luca Dalle</creator><creator>Donatelli, Luca</creator><creator>Bertoldo, Francesco</creator><creator>Zanatta, Mirko</creator><creator>Lo Cascio, Vincenzo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells</title><author>Valenti, Maria Teresa ; Carbonare, Luca Dalle ; Donatelli, Luca ; Bertoldo, Francesco ; Zanatta, Mirko ; Lo Cascio, Vincenzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-36dee14d236677788c76dcc6c53ea9cfb5b1e6c3595a72d011dee414e80990a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>CFU-F</topic><topic>Differentiation</topic><topic>Diseases of the osteoarticular system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression analysis</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Orthopedics</topic><topic>Osteoblast</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transcription Factors - genetics</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valenti, Maria Teresa</creatorcontrib><creatorcontrib>Carbonare, Luca Dalle</creatorcontrib><creatorcontrib>Donatelli, Luca</creatorcontrib><creatorcontrib>Bertoldo, Francesco</creatorcontrib><creatorcontrib>Zanatta, Mirko</creatorcontrib><creatorcontrib>Lo Cascio, Vincenzo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bone (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valenti, Maria Teresa</au><au>Carbonare, Luca Dalle</au><au>Donatelli, Luca</au><au>Bertoldo, Francesco</au><au>Zanatta, Mirko</au><au>Lo Cascio, Vincenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells</atitle><jtitle>Bone (New York, N.Y.)</jtitle><addtitle>Bone</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>43</volume><issue>6</issue><spage>1084</spage><epage>1092</epage><pages>1084-1092</pages><issn>8756-3282</issn><eissn>1873-2763</eissn><abstract>Abstract MSCs are known to have an extensive proliferative potential and ability to differentiate in various cell types. Osteoblastic differentiation from mesenchymal progenitor cells is an important step of bone formation, though the pattern of gene expression during differentiation is not yet well understood. Here, to investigate the possibility to obtain a model for in vitro bone differentiation using mesenchymal stem cells (hMSCs) from human subjects non-invasively, we developed a method to obtain hMSCs-like cells from peripheral blood by a two step method that included an enrichment of mononuclear cells followed by depletion of unwanted cells. Using these cells, we analyzed the expression of transcription factor genes (runt-related transcription factor 2 ( RUNX2 ) and osterix ( SP7 )) and bone related genes (osteopontin ( SPP1 ), osteonectin ( SPARC ) and collagen, type I, alpha 1 ( COLIA1 )) during osteoblastic differentiation. 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| subjects | Biological and medical sciences Cell Differentiation Cells, Cultured CFU-F Differentiation Diseases of the osteoarticular system Fundamental and applied biological sciences. Psychology Gene expression analysis Gene Expression Profiling Humans Immunohistochemistry Medical sciences Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Orthopedics Osteoblast Osteoblasts - cytology Osteoblasts - metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription Factors - genetics Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Gene expression analysis in osteoblastic differentiation from peripheral blood mesenchymal stem cells |
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