Construction of spectral sensitivity function using polychromatic UV sources

A procedure is presented for constructing the spectral sensitivity functions of biological dosimeters, using five polychromatic UV sources possessing different emission spectra. Phage T7 and uracil biological dosimeters have been used for measuring the dose rates of the lamps. Their spectral sensiti...

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Veröffentlicht in:Journal of photochemistry and photobiology. B, Biology Biology, 1999-04, Vol.49 (2), p.171-176
Hauptverfasser: Modos, K., Gaspar, S., Kirsch, P., Gay, M., Ronto, Gy
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container_issue 2
container_start_page 171
container_title Journal of photochemistry and photobiology. B, Biology
container_volume 49
creator Modos, K.
Gaspar, S.
Kirsch, P.
Gay, M.
Ronto, Gy
description A procedure is presented for constructing the spectral sensitivity functions of biological dosimeters, using five polychromatic UV sources possessing different emission spectra. Phage T7 and uracil biological dosimeters have been used for measuring the dose rates of the lamps. Their spectral sensitivity functions consisting of two exponential terms have been constructed. The parameters of the spectral sensitivity functions have been determined by comparing the directly measured and calculated dose-rate values. The parameters of the sensitivity function are accepted as correct values when the deviation of the measured and calculated values is a minimum. Based on the deviations between the constructed and the experimentally determined spectral sensitivities with monochromatic sources, the differences between the measured and calculated results are interpreted. The importance of the correct spectral sensitivity data is demonstrated through the effectiveness spectra of a TL 01 amp for phage T7 killing, uracil dimerization and erythema induction.
doi_str_mv 10.1016/S1011-1344(99)00052-4
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subjects Bacteriophage T7 - radiation effects
Calibration
Construction of sensitivity function
Effectiveness spectrum
Light
Polychromatic UV sources
Radiometry - standards
Spectral sensitivity of biological dosimeters
Spectrophotometry, Ultraviolet
Ultraviolet Rays
Uracil - radiation effects
title Construction of spectral sensitivity function using polychromatic UV sources
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