Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen
Anaerobic fungi (Neocallimastigales) are active degraders of fibrous plant material in the rumen. However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by ana...
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| Veröffentlicht in: | FEMS microbiology ecology 2008-12, Vol.66 (3), p.537-545 |
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| creator | Edwards, Joan E Kingston-Smith, Alison H Jimenez, Hugo R Huws, Sharon A Skøt, Kirsten P Griffith, Gareth W McEwan, Neil R Theodorou, Michael K |
| description | Anaerobic fungi (Neocallimastigales) are active degraders of fibrous plant material in the rumen. However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. Neocallimastigales-specific molecular techniques were optimized to ensure maximal coverage before application to assess the population size (quantitative PCR) and composition (automated ribosomal intergenic spacer analysis) of the colonizing anaerobic fungi. Colonization of perennial ryegrass (PRG) was evident within 5 min, with no consistent effect of time or wash procedure on fungal population composition. Wash procedure had no effect on population size unlike time, which had a significant effect. Colonizing fungal population size continued to increase over the incubation period after an initial lag of c. 4 min. This dynamic differs from that reported previously for rumen bacteria, where substantial colonization of PRG occurred within 5 min. The observed delay in colonization of plant material by anaerobic fungi is suggested to be primarily mediated by the time taken for fungal zoospores to locate, attach and encyst on plant material. |
| doi_str_mv | 10.1111/j.1574-6941.2008.00563.x |
| format | Article |
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However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. Neocallimastigales-specific molecular techniques were optimized to ensure maximal coverage before application to assess the population size (quantitative PCR) and composition (automated ribosomal intergenic spacer analysis) of the colonizing anaerobic fungi. Colonization of perennial ryegrass (PRG) was evident within 5 min, with no consistent effect of time or wash procedure on fungal population composition. Wash procedure had no effect on population size unlike time, which had a significant effect. Colonizing fungal population size continued to increase over the incubation period after an initial lag of c. 4 min. This dynamic differs from that reported previously for rumen bacteria, where substantial colonization of PRG occurred within 5 min. The observed delay in colonization of plant material by anaerobic fungi is suggested to be primarily mediated by the time taken for fungal zoospores to locate, attach and encyst on plant material.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2008.00563.x</identifier><identifier>PMID: 18673390</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>5.8S rRNA gene ; Animals ; Cattle - metabolism ; Cattle - microbiology ; Cluster Analysis ; Colonization ; Colony Count, Microbial ; Composition ; Ecology ; Female ; Fungi ; Gastrointestinal Contents - microbiology ; ITS1 ; Lolium - microbiology ; Lolium perenne ; Microbiology ; Microorganisms ; Molecular Sequence Data ; Neocallimastigales ; Neocallimastigales - growth & development ; Neocallimastigales - isolation & purification ; Neocallimastigales - physiology ; Polymerase Chain Reaction ; Population ; Population number ; Reproducibility of Results ; Response time ; Rumen ; Rumen - metabolism ; Rumen - microbiology ; Zoospores</subject><ispartof>FEMS microbiology ecology, 2008-12, Vol.66 (3), p.537-545</ispartof><rights>2008 Federation of European Microbiological Societies 2008</rights><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2008 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5603-4dc85d8fd631e00f51896642353f8f68cebec0c53277d5bf23742180e2e7dd413</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2008.00563.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2008.00563.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18673390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Edwards, Joan E</creatorcontrib><creatorcontrib>Kingston-Smith, Alison H</creatorcontrib><creatorcontrib>Jimenez, Hugo R</creatorcontrib><creatorcontrib>Huws, Sharon A</creatorcontrib><creatorcontrib>Skøt, Kirsten P</creatorcontrib><creatorcontrib>Griffith, Gareth W</creatorcontrib><creatorcontrib>McEwan, Neil R</creatorcontrib><creatorcontrib>Theodorou, Michael K</creatorcontrib><title>Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Anaerobic fungi (Neocallimastigales) are active degraders of fibrous plant material in the rumen. However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. Neocallimastigales-specific molecular techniques were optimized to ensure maximal coverage before application to assess the population size (quantitative PCR) and composition (automated ribosomal intergenic spacer analysis) of the colonizing anaerobic fungi. Colonization of perennial ryegrass (PRG) was evident within 5 min, with no consistent effect of time or wash procedure on fungal population composition. Wash procedure had no effect on population size unlike time, which had a significant effect. Colonizing fungal population size continued to increase over the incubation period after an initial lag of c. 4 min. This dynamic differs from that reported previously for rumen bacteria, where substantial colonization of PRG occurred within 5 min. The observed delay in colonization of plant material by anaerobic fungi is suggested to be primarily mediated by the time taken for fungal zoospores to locate, attach and encyst on plant material.</description><subject>5.8S rRNA gene</subject><subject>Animals</subject><subject>Cattle - metabolism</subject><subject>Cattle - microbiology</subject><subject>Cluster Analysis</subject><subject>Colonization</subject><subject>Colony Count, Microbial</subject><subject>Composition</subject><subject>Ecology</subject><subject>Female</subject><subject>Fungi</subject><subject>Gastrointestinal Contents - microbiology</subject><subject>ITS1</subject><subject>Lolium - microbiology</subject><subject>Lolium perenne</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Molecular Sequence Data</subject><subject>Neocallimastigales</subject><subject>Neocallimastigales - growth & development</subject><subject>Neocallimastigales - isolation & purification</subject><subject>Neocallimastigales - physiology</subject><subject>Polymerase Chain Reaction</subject><subject>Population</subject><subject>Population number</subject><subject>Reproducibility of Results</subject><subject>Response time</subject><subject>Rumen</subject><subject>Rumen - metabolism</subject><subject>Rumen - microbiology</subject><subject>Zoospores</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkl2L1DAUhoso7rj6FzQgeNd60nw0BW9k3VVhxQvd65Cmp2NKJxmT6e7WX2_qrAqKmJuEnOc5kLynKAiFiub1cqyoaHgpW06rGkBVAEKy6vZesflVuF9sgEpVSt7Kk-JRSiMAFYzDw-KEKtkw1sKmGN8s3uycTSQMxHl3cGYiNkzBu2_m4IJf733wNviE8Rp7sseI3q9YXHAbTUqkW4jxBmPonCXD7LcutyKHL0i6cO08kjjv0D8uHgxmSvjkbj8tri7OP5-9Ky8_vn1_9vqytEICK3lvlejV0EtGEWAQVLVS8poJNqhBKosdWrCC1U3Ti26oWcNrqgBrbPqeU3ZavDj23cfwdcZ00DuXLE6T8RjmpGWrJG_E_0HaNm0DQmTw-R_gGObo8yN0zUAKDgJW6ukdNXc77PU-up2Ji_752Rl4dQRu3ITL7zroNVQ96jU7vWan11D1j1D1rb44_5APWWdHPcz7f8jlX3K2nh2twQRtttElffWpBsryMEiaR4J9B_9OrN8</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Edwards, Joan E</creator><creator>Kingston-Smith, Alison H</creator><creator>Jimenez, Hugo R</creator><creator>Huws, Sharon A</creator><creator>Skøt, Kirsten P</creator><creator>Griffith, Gareth W</creator><creator>McEwan, Neil R</creator><creator>Theodorou, Michael K</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200812</creationdate><title>Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen</title><author>Edwards, Joan E ; Kingston-Smith, Alison H ; Jimenez, Hugo R ; Huws, Sharon A ; Skøt, Kirsten P ; Griffith, Gareth W ; McEwan, Neil R ; Theodorou, Michael K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5603-4dc85d8fd631e00f51896642353f8f68cebec0c53277d5bf23742180e2e7dd413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>5.8S rRNA gene</topic><topic>Animals</topic><topic>Cattle - 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Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Edwards, Joan E</au><au>Kingston-Smith, Alison H</au><au>Jimenez, Hugo R</au><au>Huws, Sharon A</au><au>Skøt, Kirsten P</au><au>Griffith, Gareth W</au><au>McEwan, Neil R</au><au>Theodorou, Michael K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2008-12</date><risdate>2008</risdate><volume>66</volume><issue>3</issue><spage>537</spage><epage>545</epage><pages>537-545</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Anaerobic fungi (Neocallimastigales) are active degraders of fibrous plant material in the rumen. However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. Neocallimastigales-specific molecular techniques were optimized to ensure maximal coverage before application to assess the population size (quantitative PCR) and composition (automated ribosomal intergenic spacer analysis) of the colonizing anaerobic fungi. Colonization of perennial ryegrass (PRG) was evident within 5 min, with no consistent effect of time or wash procedure on fungal population composition. Wash procedure had no effect on population size unlike time, which had a significant effect. Colonizing fungal population size continued to increase over the incubation period after an initial lag of c. 4 min. This dynamic differs from that reported previously for rumen bacteria, where substantial colonization of PRG occurred within 5 min. The observed delay in colonization of plant material by anaerobic fungi is suggested to be primarily mediated by the time taken for fungal zoospores to locate, attach and encyst on plant material.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18673390</pmid><doi>10.1111/j.1574-6941.2008.00563.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford Journals Open Access Collection |
| subjects | 5.8S rRNA gene Animals Cattle - metabolism Cattle - microbiology Cluster Analysis Colonization Colony Count, Microbial Composition Ecology Female Fungi Gastrointestinal Contents - microbiology ITS1 Lolium - microbiology Lolium perenne Microbiology Microorganisms Molecular Sequence Data Neocallimastigales Neocallimastigales - growth & development Neocallimastigales - isolation & purification Neocallimastigales - physiology Polymerase Chain Reaction Population Population number Reproducibility of Results Response time Rumen Rumen - metabolism Rumen - microbiology Zoospores |
| title | Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen |
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