Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells
Background: Transplantation of bone marrow mesenchymal stem cells (MSCs) is believed to be a new modality for periodontal tissue regeneration. However, little is known about the factors that influence the functions of the transplanted MSCs. Periodontal ligament cells may be implicated in regulating...
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| Veröffentlicht in: | Journal of periodontology (1970) 2008-12, Vol.79 (12), p.2361-2370 |
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| container_title | Journal of periodontology (1970) |
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| creator | Mizuno, Noriyoshi Ozeki, Yoshitaka Shiba, Hideki Kajiya, Mikihito Nagahara, Takayoshi Takeda, Katsuhiro Kawaguchi, Hiroyuki Abiko, Yoshimitsu Kurihara, Hidemi |
| description | Background: Transplantation of bone marrow mesenchymal stem cells (MSCs) is believed to be a new modality for periodontal tissue regeneration. However, little is known about the factors that influence the functions of the transplanted MSCs. Periodontal ligament cells may be implicated in regulating MSC functions.
Methods: To examine the effect of humoral factors (HFs) produced by human periodontal ligament (HPL) cells on human MSC (hMSC) gene expression, hMSCs were cocultured separately with HPL cells. The gene expression of the hMSCs was analyzed by microarray. Moreover, the effect of conditioned medium (CM) from cultures of HPL cells (CM‐HPL cells) on the proliferation and mineralizing capacity of hMSCs was examined.
Results: Thirty‐five genes whose expressions were upregulated more than two‐fold and 32 genes whose expressions were downregulated more than two‐fold were identified in hMSCs cocultured separately with HPL cells. CM‐HPL cells prevented calcification in hMSCs cultured with bone morphogenetic protein‐2 but not dexamethasone. CM‐HPL cells stimulated cell proliferation in hMSCs, whereas CM from culture of human osteoblasts did not influence the proliferation.
Conclusions: To the best of our knowledge, this is the first study on the global gene expression of MSCs cocultured with periodontal ligament cells. A particular humoral factor released from periodontal ligament cells is suggested to affect differentiation and proliferation in MSCs. |
| doi_str_mv | 10.1902/jop.2008.070577 |
| format | Article |
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Methods: To examine the effect of humoral factors (HFs) produced by human periodontal ligament (HPL) cells on human MSC (hMSC) gene expression, hMSCs were cocultured separately with HPL cells. The gene expression of the hMSCs was analyzed by microarray. Moreover, the effect of conditioned medium (CM) from cultures of HPL cells (CM‐HPL cells) on the proliferation and mineralizing capacity of hMSCs was examined.
Results: Thirty‐five genes whose expressions were upregulated more than two‐fold and 32 genes whose expressions were downregulated more than two‐fold were identified in hMSCs cocultured separately with HPL cells. CM‐HPL cells prevented calcification in hMSCs cultured with bone morphogenetic protein‐2 but not dexamethasone. CM‐HPL cells stimulated cell proliferation in hMSCs, whereas CM from culture of human osteoblasts did not influence the proliferation.
Conclusions: To the best of our knowledge, this is the first study on the global gene expression of MSCs cocultured with periodontal ligament cells. A particular humoral factor released from periodontal ligament cells is suggested to affect differentiation and proliferation in MSCs.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1902/jop.2008.070577</identifier><identifier>PMID: 19053928</identifier><language>eng</language><publisher>Chicago, IL: American Academy of Periodontology</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Bone marrow ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - physiology ; Bone Morphogenetic Protein 2 - pharmacology ; Calcification, Physiologic - drug effects ; Calcification, Physiologic - physiology ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Coculture Techniques ; Culture Media, Conditioned ; Dentistry ; Dexamethasone - pharmacology ; Down-Regulation ; Female ; Gene Expression ; Glucocorticoids - pharmacology ; Humans ; Inhibitor of Differentiation Protein 1 - analysis ; Inhibitor of Differentiation Proteins - analysis ; Leptin - analysis ; Male ; Medical sciences ; mesenchymal stem cells ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - physiology ; Microarray Analysis ; Neoplasm Proteins - analysis ; Osteoblasts - drug effects ; Osteoblasts - physiology ; Otorhinolaryngology. Stomatology ; periodontal ligament ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Periodontal Ligament - physiology ; Up-Regulation</subject><ispartof>Journal of periodontology (1970), 2008-12, Vol.79 (12), p.2361-2370</ispartof><rights>2008 American Academy of Periodontology</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4371-fe118f9aeae835886d094111b00f67751a06de39d8bb24c9482741e4a387ad2b3</citedby><cites>FETCH-LOGICAL-c4371-fe118f9aeae835886d094111b00f67751a06de39d8bb24c9482741e4a387ad2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1902%2Fjop.2008.070577$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1902%2Fjop.2008.070577$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20971514$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19053928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizuno, Noriyoshi</creatorcontrib><creatorcontrib>Ozeki, Yoshitaka</creatorcontrib><creatorcontrib>Shiba, Hideki</creatorcontrib><creatorcontrib>Kajiya, Mikihito</creatorcontrib><creatorcontrib>Nagahara, Takayoshi</creatorcontrib><creatorcontrib>Takeda, Katsuhiro</creatorcontrib><creatorcontrib>Kawaguchi, Hiroyuki</creatorcontrib><creatorcontrib>Abiko, Yoshimitsu</creatorcontrib><creatorcontrib>Kurihara, Hidemi</creatorcontrib><title>Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background: Transplantation of bone marrow mesenchymal stem cells (MSCs) is believed to be a new modality for periodontal tissue regeneration. However, little is known about the factors that influence the functions of the transplanted MSCs. Periodontal ligament cells may be implicated in regulating MSC functions.
Methods: To examine the effect of humoral factors (HFs) produced by human periodontal ligament (HPL) cells on human MSC (hMSC) gene expression, hMSCs were cocultured separately with HPL cells. The gene expression of the hMSCs was analyzed by microarray. Moreover, the effect of conditioned medium (CM) from cultures of HPL cells (CM‐HPL cells) on the proliferation and mineralizing capacity of hMSCs was examined.
Results: Thirty‐five genes whose expressions were upregulated more than two‐fold and 32 genes whose expressions were downregulated more than two‐fold were identified in hMSCs cocultured separately with HPL cells. CM‐HPL cells prevented calcification in hMSCs cultured with bone morphogenetic protein‐2 but not dexamethasone. CM‐HPL cells stimulated cell proliferation in hMSCs, whereas CM from culture of human osteoblasts did not influence the proliferation.
Conclusions: To the best of our knowledge, this is the first study on the global gene expression of MSCs cocultured with periodontal ligament cells. A particular humoral factor released from periodontal ligament cells is suggested to affect differentiation and proliferation in MSCs.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - physiology</subject><subject>Bone Morphogenetic Protein 2 - pharmacology</subject><subject>Calcification, Physiologic - drug effects</subject><subject>Calcification, Physiologic - physiology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Culture Media, Conditioned</subject><subject>Dentistry</subject><subject>Dexamethasone - pharmacology</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Glucocorticoids - pharmacology</subject><subject>Humans</subject><subject>Inhibitor of Differentiation Protein 1 - analysis</subject><subject>Inhibitor of Differentiation Proteins - analysis</subject><subject>Leptin - analysis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Microarray Analysis</subject><subject>Neoplasm Proteins - analysis</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - physiology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Periodontal Ligament - physiology</subject><subject>Up-Regulation</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EosPAmh3yhu4y9U8SO0sYdWjRVIwKrKMb5wZcOfZgJ6rmPXhgXGUES1aW7fOda_kj5C1nG94wcfUQjhvBmN4wxSqlnpEVb0pZyFqx52TFmBCFLBtxQV6l9JC3vJTsJbnI2Uo2Qq_I75t5DBEc3YGZQkz0Hh1Cwp7uYhhpvgVPDxht6IOfMre3P2BEP9EtOpforR_cjN4g3YIzdrAGJhs8Bd_TQwzODhiXE-vPto_BI72DGMMjvcOUwz9PYzZ_nXBcrK_JiwFcwjfndU2-766_bW-K_ZdPt9sP-8KUUvFiQM710AACallpXfesKTnnHWNDrVTFgdU9yqbXXSdK05RaqJJjCVIr6EUn1-Ry8R5j-DVjmtrRJpNfAB7DnNq60bXUUmXwagFNDClFHNpjtCPEU8tZ-1REm4ton4polyJy4t1ZPXcj9v_4889n4P0ZgGTADRG8sekvJ1ijeJXrWpNq4R6tw9P_5rafD9f3QtZc_gEIE6LN</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Mizuno, Noriyoshi</creator><creator>Ozeki, Yoshitaka</creator><creator>Shiba, Hideki</creator><creator>Kajiya, Mikihito</creator><creator>Nagahara, Takayoshi</creator><creator>Takeda, Katsuhiro</creator><creator>Kawaguchi, Hiroyuki</creator><creator>Abiko, Yoshimitsu</creator><creator>Kurihara, Hidemi</creator><general>American Academy of Periodontology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200812</creationdate><title>Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells</title><author>Mizuno, Noriyoshi ; Ozeki, Yoshitaka ; Shiba, Hideki ; Kajiya, Mikihito ; Nagahara, Takayoshi ; Takeda, Katsuhiro ; Kawaguchi, Hiroyuki ; Abiko, Yoshimitsu ; Kurihara, Hidemi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4371-fe118f9aeae835886d094111b00f67751a06de39d8bb24c9482741e4a387ad2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - physiology</topic><topic>Bone Morphogenetic Protein 2 - pharmacology</topic><topic>Calcification, Physiologic - drug effects</topic><topic>Calcification, Physiologic - physiology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Culture Media, Conditioned</topic><topic>Dentistry</topic><topic>Dexamethasone - pharmacology</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Glucocorticoids - pharmacology</topic><topic>Humans</topic><topic>Inhibitor of Differentiation Protein 1 - analysis</topic><topic>Inhibitor of Differentiation Proteins - analysis</topic><topic>Leptin - analysis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Microarray Analysis</topic><topic>Neoplasm Proteins - analysis</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - physiology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Periodontal Ligament - physiology</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizuno, Noriyoshi</creatorcontrib><creatorcontrib>Ozeki, Yoshitaka</creatorcontrib><creatorcontrib>Shiba, Hideki</creatorcontrib><creatorcontrib>Kajiya, Mikihito</creatorcontrib><creatorcontrib>Nagahara, Takayoshi</creatorcontrib><creatorcontrib>Takeda, Katsuhiro</creatorcontrib><creatorcontrib>Kawaguchi, Hiroyuki</creatorcontrib><creatorcontrib>Abiko, Yoshimitsu</creatorcontrib><creatorcontrib>Kurihara, Hidemi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizuno, Noriyoshi</au><au>Ozeki, Yoshitaka</au><au>Shiba, Hideki</au><au>Kajiya, Mikihito</au><au>Nagahara, Takayoshi</au><au>Takeda, Katsuhiro</au><au>Kawaguchi, Hiroyuki</au><au>Abiko, Yoshimitsu</au><au>Kurihara, Hidemi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2008-12</date><risdate>2008</risdate><volume>79</volume><issue>12</issue><spage>2361</spage><epage>2370</epage><pages>2361-2370</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background: Transplantation of bone marrow mesenchymal stem cells (MSCs) is believed to be a new modality for periodontal tissue regeneration. However, little is known about the factors that influence the functions of the transplanted MSCs. Periodontal ligament cells may be implicated in regulating MSC functions.
Methods: To examine the effect of humoral factors (HFs) produced by human periodontal ligament (HPL) cells on human MSC (hMSC) gene expression, hMSCs were cocultured separately with HPL cells. The gene expression of the hMSCs was analyzed by microarray. Moreover, the effect of conditioned medium (CM) from cultures of HPL cells (CM‐HPL cells) on the proliferation and mineralizing capacity of hMSCs was examined.
Results: Thirty‐five genes whose expressions were upregulated more than two‐fold and 32 genes whose expressions were downregulated more than two‐fold were identified in hMSCs cocultured separately with HPL cells. CM‐HPL cells prevented calcification in hMSCs cultured with bone morphogenetic protein‐2 but not dexamethasone. CM‐HPL cells stimulated cell proliferation in hMSCs, whereas CM from culture of human osteoblasts did not influence the proliferation.
Conclusions: To the best of our knowledge, this is the first study on the global gene expression of MSCs cocultured with periodontal ligament cells. A particular humoral factor released from periodontal ligament cells is suggested to affect differentiation and proliferation in MSCs.</abstract><cop>Chicago, IL</cop><pub>American Academy of Periodontology</pub><pmid>19053928</pmid><doi>10.1902/jop.2008.070577</doi><tpages>10</tpages></addata></record> |
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| subjects | Adolescent Adult Biological and medical sciences Bone marrow Bone Marrow Cells - drug effects Bone Marrow Cells - physiology Bone Morphogenetic Protein 2 - pharmacology Calcification, Physiologic - drug effects Calcification, Physiologic - physiology Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Coculture Techniques Culture Media, Conditioned Dentistry Dexamethasone - pharmacology Down-Regulation Female Gene Expression Glucocorticoids - pharmacology Humans Inhibitor of Differentiation Protein 1 - analysis Inhibitor of Differentiation Proteins - analysis Leptin - analysis Male Medical sciences mesenchymal stem cells Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - physiology Microarray Analysis Neoplasm Proteins - analysis Osteoblasts - drug effects Osteoblasts - physiology Otorhinolaryngology. Stomatology periodontal ligament Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - physiology Up-Regulation |
| title | Humoral Factors Released From Human Periodontal Ligament Cells Influence Calcification and Proliferation in Human Bone Marrow Mesenchymal Stem Cells |
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