In vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of Malassezia species
Abstract Malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. We hypothesize that the lipid-rich capsule of Malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. To test this, preparations of cap...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2008-11, Vol.54 (2), p.203-214 |
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| creator | Thomas, Daniel S. Ingham, Eileen Bojar, Richard A. Holland, Keith T. |
| description | Abstract
Malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. We hypothesize that the lipid-rich capsule of Malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. To test this, preparations of capsulated or acapsular [10% (v/v) Triton X-100 treated], viable and nonviable, exponential or stationary phase Malassezia furfur, Malassezia globosa, Malassezia obtusa, Malassezia restricta, Malassezia slooffiae and Malassezia sympodialis, were incubated with normal human keratinocytes. Proinflammatory (IL-6, IL-8, IL-1α and tumour necrosis factor-α) and anti-inflammatory cytokine (IL-10) release and intracellular IL-10 concentrations were quantified using enzyme-linked immunosorbent assays. Capsulated Malassezia yeasts stimulated limited or no production of inflammatory cytokines, and increased intracellular IL-10 (P |
| doi_str_mv | 10.1111/j.1574-695X.2008.00468.x |
| format | Article |
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Malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. We hypothesize that the lipid-rich capsule of Malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. To test this, preparations of capsulated or acapsular [10% (v/v) Triton X-100 treated], viable and nonviable, exponential or stationary phase Malassezia furfur, Malassezia globosa, Malassezia obtusa, Malassezia restricta, Malassezia slooffiae and Malassezia sympodialis, were incubated with normal human keratinocytes. Proinflammatory (IL-6, IL-8, IL-1α and tumour necrosis factor-α) and anti-inflammatory cytokine (IL-10) release and intracellular IL-10 concentrations were quantified using enzyme-linked immunosorbent assays. Capsulated Malassezia yeasts stimulated limited or no production of inflammatory cytokines, and increased intracellular IL-10 (P<0.05). Removal of the capsule of many Malassezia preparations caused a significantly increased production of IL-6, IL-8 and IL-1α, and a decrease in intracellular IL-10. Notably, acapsular viable, stationary phase M. globosa caused a 66-fold increase in IL-8 production (P<0.001) and acapsular nonviable, stationary phase M. furfur caused a 38-fold increase in IL-6 production (P<0.001) and a 12-fold decrease in intracellular IL-10 (P<0.001). These results support the hypothesis that the lipid layer of Malassezia spp. modulates cytokine production by keratinocytes. This has implications in the pathogenesis of seborrhoeic dermatitis.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.2008.00468.x</identifier><identifier>PMID: 18752620</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Analysis of Variance ; Biological and medical sciences ; capsule ; Cell Survival ; Cell Wall - physiology ; Cells, Cultured ; Cytokines ; Depletion ; Dermatitis ; Dermatomycoses - microbiology ; Fundamental and applied biological sciences. Psychology ; Fungi ; Humans ; Immunoassays ; immunoevasion ; Inflammation ; Inflammatory response ; Interleukin 10 ; Interleukin 6 ; Interleukin 8 ; Interleukins - biosynthesis ; Intracellular ; Keratinocytes ; Keratinocytes - cytology ; Keratinocytes - metabolism ; Lipids ; Lipids - physiology ; Malassezia ; Malassezia - physiology ; Malassezia furfur ; Malassezia sympodialis ; Masks ; Microbiology ; Microscopy, Electron, Transmission ; Miscellaneous ; Mycology ; Pathogenesis ; seborrheic dermatitis ; Skin - microbiology ; Stationary phase ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumor necrosis factor-α ; Tumors ; Yeast ; Yeasts</subject><ispartof>FEMS immunology and medical microbiology, 2008-11, Vol.54 (2), p.203-214</ispartof><rights>2008 Federation of European Microbiological Societies. 2008</rights><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2008 INIST-CNRS</rights><rights>2008 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4998-2e438fb9e5450b896b07b5daa07673e227e8ff41d0948d4f99c762d740977da3</citedby><cites>FETCH-LOGICAL-c4998-2e438fb9e5450b896b07b5daa07673e227e8ff41d0948d4f99c762d740977da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.2008.00468.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.2008.00468.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20759685$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18752620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, Daniel S.</creatorcontrib><creatorcontrib>Ingham, Eileen</creatorcontrib><creatorcontrib>Bojar, Richard A.</creatorcontrib><creatorcontrib>Holland, Keith T.</creatorcontrib><title>In vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of Malassezia species</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Abstract
Malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. We hypothesize that the lipid-rich capsule of Malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. To test this, preparations of capsulated or acapsular [10% (v/v) Triton X-100 treated], viable and nonviable, exponential or stationary phase Malassezia furfur, Malassezia globosa, Malassezia obtusa, Malassezia restricta, Malassezia slooffiae and Malassezia sympodialis, were incubated with normal human keratinocytes. Proinflammatory (IL-6, IL-8, IL-1α and tumour necrosis factor-α) and anti-inflammatory cytokine (IL-10) release and intracellular IL-10 concentrations were quantified using enzyme-linked immunosorbent assays. Capsulated Malassezia yeasts stimulated limited or no production of inflammatory cytokines, and increased intracellular IL-10 (P<0.05). Removal of the capsule of many Malassezia preparations caused a significantly increased production of IL-6, IL-8 and IL-1α, and a decrease in intracellular IL-10. Notably, acapsular viable, stationary phase M. globosa caused a 66-fold increase in IL-8 production (P<0.001) and acapsular nonviable, stationary phase M. furfur caused a 38-fold increase in IL-6 production (P<0.001) and a 12-fold decrease in intracellular IL-10 (P<0.001). These results support the hypothesis that the lipid layer of Malassezia spp. modulates cytokine production by keratinocytes. This has implications in the pathogenesis of seborrhoeic dermatitis.</description><subject>Analysis of Variance</subject><subject>Biological and medical sciences</subject><subject>capsule</subject><subject>Cell Survival</subject><subject>Cell Wall - physiology</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>Depletion</subject><subject>Dermatitis</subject><subject>Dermatomycoses - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Humans</subject><subject>Immunoassays</subject><subject>immunoevasion</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 10</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Interleukins - biosynthesis</subject><subject>Intracellular</subject><subject>Keratinocytes</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - metabolism</subject><subject>Lipids</subject><subject>Lipids - physiology</subject><subject>Malassezia</subject><subject>Malassezia - physiology</subject><subject>Malassezia furfur</subject><subject>Malassezia sympodialis</subject><subject>Masks</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Transmission</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Pathogenesis</subject><subject>seborrheic dermatitis</subject><subject>Skin - microbiology</subject><subject>Stationary phase</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumor necrosis factor-α</subject><subject>Tumors</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9r1TAYxoMo7jj9ChIQvWtN0_wFb2Q4PbDhzS68C2maspy1SU3auQr77kvPOUxQRAMhL3l_z5M3PADACpVVXu93ZUU5KZik30qMkCgRIkyUd0_A5rHxFGyQxKIQmJAT8CKlHcqUROg5OKkEp5hhtAH3Ww9v3RQDHEI793pywcPQwet50B7e2JhvfDDLZOEYQwG1b_OeXOF81-th0FOIC8z9cOP8nmlnszdpFjhdW2j0mOberp6Xutcp2Z9OwzRa42x6CZ51uk_21fE8BVfnn67OvhQXXz9vzz5eFIZIKQpsSS26RlpKKGqEZA3iDW21Rpzx2mLMreg6UrVIEtGSTkrDGW45QZLzVten4N3BNo_3fbZpUoNLxva99jbMSTEpWM1J9U-wkjUWlIgMvvkN3IU5-vwHhWvEMJeUr3biQJkYUoq2U2N0g46LqpBag1Q7teal1rzUGqTaB6nusvT18YG5GWz7S3hMLgNvj4BORvdd1N649MhhxKlkgmbuw4H74Xq7_PcA6nx7mYssrw_yMI9_ERd_Tv8AePjJmA</recordid><startdate>200811</startdate><enddate>200811</enddate><creator>Thomas, Daniel S.</creator><creator>Ingham, Eileen</creator><creator>Bojar, Richard A.</creator><creator>Holland, Keith T.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200811</creationdate><title>In vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of Malassezia species</title><author>Thomas, Daniel S. ; Ingham, Eileen ; Bojar, Richard A. ; Holland, Keith T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4998-2e438fb9e5450b896b07b5daa07673e227e8ff41d0948d4f99c762d740977da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Analysis of Variance</topic><topic>Biological and medical sciences</topic><topic>capsule</topic><topic>Cell Survival</topic><topic>Cell Wall - physiology</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>Depletion</topic><topic>Dermatitis</topic><topic>Dermatomycoses - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Humans</topic><topic>Immunoassays</topic><topic>immunoevasion</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Interleukin 10</topic><topic>Interleukin 6</topic><topic>Interleukin 8</topic><topic>Interleukins - biosynthesis</topic><topic>Intracellular</topic><topic>Keratinocytes</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - metabolism</topic><topic>Lipids</topic><topic>Lipids - physiology</topic><topic>Malassezia</topic><topic>Malassezia - physiology</topic><topic>Malassezia furfur</topic><topic>Malassezia sympodialis</topic><topic>Masks</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Transmission</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Pathogenesis</topic><topic>seborrheic dermatitis</topic><topic>Skin - microbiology</topic><topic>Stationary phase</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumor necrosis factor-α</topic><topic>Tumors</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, Daniel S.</creatorcontrib><creatorcontrib>Ingham, Eileen</creatorcontrib><creatorcontrib>Bojar, Richard A.</creatorcontrib><creatorcontrib>Holland, Keith T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, Daniel S.</au><au>Ingham, Eileen</au><au>Bojar, Richard A.</au><au>Holland, Keith T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of Malassezia species</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2008-11</date><risdate>2008</risdate><volume>54</volume><issue>2</issue><spage>203</spage><epage>214</epage><pages>203-214</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Abstract
Malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. We hypothesize that the lipid-rich capsule of Malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. To test this, preparations of capsulated or acapsular [10% (v/v) Triton X-100 treated], viable and nonviable, exponential or stationary phase Malassezia furfur, Malassezia globosa, Malassezia obtusa, Malassezia restricta, Malassezia slooffiae and Malassezia sympodialis, were incubated with normal human keratinocytes. Proinflammatory (IL-6, IL-8, IL-1α and tumour necrosis factor-α) and anti-inflammatory cytokine (IL-10) release and intracellular IL-10 concentrations were quantified using enzyme-linked immunosorbent assays. Capsulated Malassezia yeasts stimulated limited or no production of inflammatory cytokines, and increased intracellular IL-10 (P<0.05). Removal of the capsule of many Malassezia preparations caused a significantly increased production of IL-6, IL-8 and IL-1α, and a decrease in intracellular IL-10. Notably, acapsular viable, stationary phase M. globosa caused a 66-fold increase in IL-8 production (P<0.001) and acapsular nonviable, stationary phase M. furfur caused a 38-fold increase in IL-6 production (P<0.001) and a 12-fold decrease in intracellular IL-10 (P<0.001). These results support the hypothesis that the lipid layer of Malassezia spp. modulates cytokine production by keratinocytes. This has implications in the pathogenesis of seborrhoeic dermatitis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18752620</pmid><doi>10.1111/j.1574-695X.2008.00468.x</doi><tpages>12</tpages></addata></record> |
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| subjects | Analysis of Variance Biological and medical sciences capsule Cell Survival Cell Wall - physiology Cells, Cultured Cytokines Depletion Dermatitis Dermatomycoses - microbiology Fundamental and applied biological sciences. Psychology Fungi Humans Immunoassays immunoevasion Inflammation Inflammatory response Interleukin 10 Interleukin 6 Interleukin 8 Interleukins - biosynthesis Intracellular Keratinocytes Keratinocytes - cytology Keratinocytes - metabolism Lipids Lipids - physiology Malassezia Malassezia - physiology Malassezia furfur Malassezia sympodialis Masks Microbiology Microscopy, Electron, Transmission Miscellaneous Mycology Pathogenesis seborrheic dermatitis Skin - microbiology Stationary phase Tumor Necrosis Factor-alpha - biosynthesis Tumor necrosis factor-α Tumors Yeast Yeasts |
| title | In vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of Malassezia species |
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