Defining a transcriptional fingerprint of murine splenic B-cell development
B-cell development occurs in a stepwise fashion that can be followed by the expression of B cell-specific surface markers. In this study, we wished to identify proteins that could contribute to the changes in expression of such markers. By using RNA from freshly isolated B220+ cells, we hoped to red...
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| Veröffentlicht in: | Genes and immunity 2008-12, Vol.9 (8), p.706-720 |
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| creator | Debnath, I Roundy, K M Dunn, D M Weiss, R B Weis, J J Weis, J H |
| description | B-cell development occurs in a stepwise fashion that can be followed by the expression of B cell-specific surface markers. In this study, we wished to identify proteins that could contribute to the changes in expression of such markers. By using RNA from freshly isolated B220+ cells, we hoped to reduce the effect of artifacts that occur during the isolation and amplification steps necessary to use flow cytometry analysis-sorted subsets in microarray experiments. Analyses comparing expression patterns from B220+ 2-week bone marrow (pro-B, pre-B, immature B cells), 2-week spleen (predominantly transitional cells) and 8-week spleen (mainly mature B cells) yielded hundreds of genes. We also examined the B cell-activating factor (BAFF)-dependent effects on immature splenic B cells by comparing expression patterns in the spleen between 2-week A/J vs 2-week A/WySnJ mice, which lack functional BAFF receptor signaling. Genes that showed the expression differences between spleen and bone marrow samples were then analyzed through quantitative PCR on B-cell subsets isolated using two different sorting protocols. A comparison of the results from our study with the results from other analyses showed not only some overlap of preferentially expressed genes but also an expansion of other genes potentially involved in B-cell development. |
| doi_str_mv | 10.1038/gene.2008.70 |
| format | Article |
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In this study, we wished to identify proteins that could contribute to the changes in expression of such markers. By using RNA from freshly isolated B220+ cells, we hoped to reduce the effect of artifacts that occur during the isolation and amplification steps necessary to use flow cytometry analysis-sorted subsets in microarray experiments. Analyses comparing expression patterns from B220+ 2-week bone marrow (pro-B, pre-B, immature B cells), 2-week spleen (predominantly transitional cells) and 8-week spleen (mainly mature B cells) yielded hundreds of genes. We also examined the B cell-activating factor (BAFF)-dependent effects on immature splenic B cells by comparing expression patterns in the spleen between 2-week A/J vs 2-week A/WySnJ mice, which lack functional BAFF receptor signaling. Genes that showed the expression differences between spleen and bone marrow samples were then analyzed through quantitative PCR on B-cell subsets isolated using two different sorting protocols. A comparison of the results from our study with the results from other analyses showed not only some overlap of preferentially expressed genes but also an expansion of other genes potentially involved in B-cell development.</description><identifier>ISSN: 1466-4879</identifier><identifier>EISSN: 1476-5470</identifier><identifier>DOI: 10.1038/gene.2008.70</identifier><identifier>PMID: 18784731</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; B cells ; B-Cell Activating Factor - genetics ; B-Cell Activating Factor - immunology ; B-Cell Activating Factor - metabolism ; B-Cell Activation Factor Receptor - genetics ; B-Cell Activation Factor Receptor - immunology ; B-Cell Activation Factor Receptor - metabolism ; B-Lymphocyte Subsets - immunology ; B-Lymphocyte Subsets - metabolism ; Biomedical and Life Sciences ; Biomedicine ; BLyS protein ; Bone marrow ; Bone Marrow Cells - immunology ; Bone Marrow Cells - metabolism ; Cancer Research ; Cell Differentiation - genetics ; Cell Differentiation - immunology ; Cells ; DNA microarrays ; Flow cytometry ; Gene Expression ; Gene Expression Profiling ; Genetic research ; Genetic transcription ; Human Genetics ; Immunology ; Kinases ; Lymphocytes B ; Mice ; Mice, Inbred Strains ; Oligonucleotide Array Sequence Analysis ; original-article ; Properties ; Proteins ; Signal Transduction ; Spleen ; Spleen - immunology ; Spleen - metabolism ; Surface markers ; Transcription ; Transcription factors ; Transcription, Genetic ; Tumor necrosis factor-TNF</subject><ispartof>Genes and immunity, 2008-12, Vol.9 (8), p.706-720</ispartof><rights>Macmillan Publishers Limited 2008</rights><rights>COPYRIGHT 2008 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Dec 2008</rights><rights>Macmillan Publishers Limited 2008.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-9a5af6995b71ad33ab9a1ed74c9f90a9a30d8e4e05e53d68ed949d2ce84ce17e3</citedby><cites>FETCH-LOGICAL-c539t-9a5af6995b71ad33ab9a1ed74c9f90a9a30d8e4e05e53d68ed949d2ce84ce17e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/gene.2008.70$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/gene.2008.70$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18784731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Debnath, I</creatorcontrib><creatorcontrib>Roundy, K M</creatorcontrib><creatorcontrib>Dunn, D M</creatorcontrib><creatorcontrib>Weiss, R B</creatorcontrib><creatorcontrib>Weis, J J</creatorcontrib><creatorcontrib>Weis, J H</creatorcontrib><title>Defining a transcriptional fingerprint of murine splenic B-cell development</title><title>Genes and immunity</title><addtitle>Genes Immun</addtitle><addtitle>Genes Immun</addtitle><description>B-cell development occurs in a stepwise fashion that can be followed by the expression of B cell-specific surface markers. In this study, we wished to identify proteins that could contribute to the changes in expression of such markers. By using RNA from freshly isolated B220+ cells, we hoped to reduce the effect of artifacts that occur during the isolation and amplification steps necessary to use flow cytometry analysis-sorted subsets in microarray experiments. Analyses comparing expression patterns from B220+ 2-week bone marrow (pro-B, pre-B, immature B cells), 2-week spleen (predominantly transitional cells) and 8-week spleen (mainly mature B cells) yielded hundreds of genes. We also examined the B cell-activating factor (BAFF)-dependent effects on immature splenic B cells by comparing expression patterns in the spleen between 2-week A/J vs 2-week A/WySnJ mice, which lack functional BAFF receptor signaling. Genes that showed the expression differences between spleen and bone marrow samples were then analyzed through quantitative PCR on B-cell subsets isolated using two different sorting protocols. A comparison of the results from our study with the results from other analyses showed not only some overlap of preferentially expressed genes but also an expansion of other genes potentially involved in B-cell development.</description><subject>Animals</subject><subject>B cells</subject><subject>B-Cell Activating Factor - genetics</subject><subject>B-Cell Activating Factor - immunology</subject><subject>B-Cell Activating Factor - metabolism</subject><subject>B-Cell Activation Factor Receptor - genetics</subject><subject>B-Cell Activation Factor Receptor - immunology</subject><subject>B-Cell Activation Factor Receptor - metabolism</subject><subject>B-Lymphocyte Subsets - immunology</subject><subject>B-Lymphocyte Subsets - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>BLyS protein</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - immunology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cancer Research</subject><subject>Cell Differentiation - 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genetics</topic><topic>B-Cell Activating Factor - immunology</topic><topic>B-Cell Activating Factor - metabolism</topic><topic>B-Cell Activation Factor Receptor - genetics</topic><topic>B-Cell Activation Factor Receptor - immunology</topic><topic>B-Cell Activation Factor Receptor - metabolism</topic><topic>B-Lymphocyte Subsets - immunology</topic><topic>B-Lymphocyte Subsets - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>BLyS protein</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - immunology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cancer Research</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Differentiation - immunology</topic><topic>Cells</topic><topic>DNA microarrays</topic><topic>Flow cytometry</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Genetic research</topic><topic>Genetic transcription</topic><topic>Human Genetics</topic><topic>Immunology</topic><topic>Kinases</topic><topic>Lymphocytes B</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>original-article</topic><topic>Properties</topic><topic>Proteins</topic><topic>Signal Transduction</topic><topic>Spleen</topic><topic>Spleen - 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Academic</collection><jtitle>Genes and immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debnath, I</au><au>Roundy, K M</au><au>Dunn, D M</au><au>Weiss, R B</au><au>Weis, J J</au><au>Weis, J H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defining a transcriptional fingerprint of murine splenic B-cell development</atitle><jtitle>Genes and immunity</jtitle><stitle>Genes Immun</stitle><addtitle>Genes Immun</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>9</volume><issue>8</issue><spage>706</spage><epage>720</epage><pages>706-720</pages><issn>1466-4879</issn><eissn>1476-5470</eissn><abstract>B-cell development occurs in a stepwise fashion that can be followed by the expression of B cell-specific surface markers. In this study, we wished to identify proteins that could contribute to the changes in expression of such markers. By using RNA from freshly isolated B220+ cells, we hoped to reduce the effect of artifacts that occur during the isolation and amplification steps necessary to use flow cytometry analysis-sorted subsets in microarray experiments. Analyses comparing expression patterns from B220+ 2-week bone marrow (pro-B, pre-B, immature B cells), 2-week spleen (predominantly transitional cells) and 8-week spleen (mainly mature B cells) yielded hundreds of genes. We also examined the B cell-activating factor (BAFF)-dependent effects on immature splenic B cells by comparing expression patterns in the spleen between 2-week A/J vs 2-week A/WySnJ mice, which lack functional BAFF receptor signaling. Genes that showed the expression differences between spleen and bone marrow samples were then analyzed through quantitative PCR on B-cell subsets isolated using two different sorting protocols. A comparison of the results from our study with the results from other analyses showed not only some overlap of preferentially expressed genes but also an expansion of other genes potentially involved in B-cell development.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>18784731</pmid><doi>10.1038/gene.2008.70</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | Animals B cells B-Cell Activating Factor - genetics B-Cell Activating Factor - immunology B-Cell Activating Factor - metabolism B-Cell Activation Factor Receptor - genetics B-Cell Activation Factor Receptor - immunology B-Cell Activation Factor Receptor - metabolism B-Lymphocyte Subsets - immunology B-Lymphocyte Subsets - metabolism Biomedical and Life Sciences Biomedicine BLyS protein Bone marrow Bone Marrow Cells - immunology Bone Marrow Cells - metabolism Cancer Research Cell Differentiation - genetics Cell Differentiation - immunology Cells DNA microarrays Flow cytometry Gene Expression Gene Expression Profiling Genetic research Genetic transcription Human Genetics Immunology Kinases Lymphocytes B Mice Mice, Inbred Strains Oligonucleotide Array Sequence Analysis original-article Properties Proteins Signal Transduction Spleen Spleen - immunology Spleen - metabolism Surface markers Transcription Transcription factors Transcription, Genetic Tumor necrosis factor-TNF |
| title | Defining a transcriptional fingerprint of murine splenic B-cell development |
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