In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system
We demonstrate the feasibility of detecting individual tumor‐infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high‐resolution surface coil. Using a recently developed high‐temperature superconducting (HTS) surface coil, single cel...
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| Veröffentlicht in: | Magnetic resonance in medicine 2008-12, Vol.60 (6), p.1292-1297 |
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| container_title | Magnetic resonance in medicine |
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| creator | Smirnov, Pierre Poirier-Quinot, Marie Wilhelm, Claire Lavergne, Elise Ginefri, Jean-Christophe Combadière, Béhazine Clément, Olivier Darrasse, Luc Gazeau, Florence |
| description | We demonstrate the feasibility of detecting individual tumor‐infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high‐resolution surface coil. Using a recently developed high‐temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 μm)3 at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin‐expressing tumors by transferred OVA antigen‐specific cytotoxic lymphocytes with low iron load. Magn Reson Med 60:1292–1297, 2008. © 2008 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/mrm.21812 |
| format | Article |
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Reson. Med</addtitle><description>We demonstrate the feasibility of detecting individual tumor‐infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high‐resolution surface coil. Using a recently developed high‐temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 μm)3 at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin‐expressing tumors by transferred OVA antigen‐specific cytotoxic lymphocytes with low iron load. 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Reson. Med</addtitle><date>2008-12</date><risdate>2008</risdate><volume>60</volume><issue>6</issue><spage>1292</spage><epage>1297</epage><pages>1292-1297</pages><issn>0740-3194</issn><eissn>1522-2594</eissn><abstract>We demonstrate the feasibility of detecting individual tumor‐infiltrating cells in vivo, by means of cellular magnetic labeling and a 1.5 Tesla clinical MRI device equipped with a high‐resolution surface coil. Using a recently developed high‐temperature superconducting (HTS) surface coil, single cells were detected in vitro in voxels of (60 μm)3 at magnetic loads as low as 0.2 pg of iron per cell. The same imaging protocol was used in vivo to monitor infiltration of ovalbumin‐expressing tumors by transferred OVA antigen‐specific cytotoxic lymphocytes with low iron load. 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| subjects | Animals cancer cell imaging Cell Line Contrast Media Equipment Design Equipment Failure Analysis Feasibility Studies Ferric Compounds high resolution MRI high-temperature superconducting coil Image Enhancement - instrumentation izon oxide nanoparticles Magnetic Resonance Imaging - instrumentation Magnetic Resonance Imaging - methods Magnetics - instrumentation Magnetics - methods Mice Mice, Inbred C57BL Neoplasms - pathology Neutrophil Infiltration T-Lymphocytes - pathology |
| title | In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system |
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