Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism

We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reac...

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Veröffentlicht in:	Molecular and cellular probes 2008-10, Vol.22 (5), p.301-304
Hauptverfasser:	Gromadzka, Beata, Smietanka, Krzysztof, Dragun, Jakub, Minta, Zenon, Gora-Sochacka, Anna, Szewczyk, Boguslaw
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Avian influenza Birds - virology DNA, Complementary - genetics Genome, Viral Genotype Humans Influenza A virus Influenza A virus - genetics Influenza in Birds - classification Influenza in Birds - diagnosis Influenza in Birds - genetics Phylogeny Polymorphism, Single-Stranded Conformational Reverse Transcriptase Polymerase Chain Reaction - methods Sequence amplification Single-stranded conformational polymorphism Temperature
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