Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism
We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reac...
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| Veröffentlicht in: | Molecular and cellular probes 2008-10, Vol.22 (5), p.301-304 |
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| creator | Gromadzka, Beata Smietanka, Krzysztof Dragun, Jakub Minta, Zenon Gora-Sochacka, Anna Szewczyk, Boguslaw |
| description | We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to electrophoresis. After silver staining, ssDNA band patterns characteristic for each genotype were observed. Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster. |
| doi_str_mv | 10.1016/j.mcp.2008.09.003 |
| format | Article |
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Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster.</description><subject>Animals</subject><subject>Avian influenza</subject><subject>Birds - virology</subject><subject>DNA, Complementary - genetics</subject><subject>Genome, Viral</subject><subject>Genotype</subject><subject>Humans</subject><subject>Influenza A virus</subject><subject>Influenza A virus - genetics</subject><subject>Influenza in Birds - classification</subject><subject>Influenza in Birds - diagnosis</subject><subject>Influenza in Birds - genetics</subject><subject>Phylogeny</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Sequence amplification</subject><subject>Single-stranded conformational polymorphism</subject><subject>Temperature</subject><issn>0890-8508</issn><issn>1096-1194</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P0zAQhi0EYrsLP4AL8olbyjhxHFuc0PKxSCtxgbPl2uOuq9gOdlKp_HpStRI3OM1o9LzvYR5C3jDYMmDi_WEb7bRtAeQW1Bage0Y2DJRoGFP8OdmAVNDIHuQNua31AACKg3xJbphU_dC1sCHHTzijnUNONHtqn0zaY6UhUXMMJq2LHxdMvw3dY8oRqS9mHzHNle5ONC7jHGaMExYzLwVpDWk_YlPnYpKjNiefSzTndjPSKY-nmMv0FGp8RV54M1Z8fZ135OeXzz_uH5rH71-_3X98bGwn27kxCtF6OSAziO3A7dAqIVRv_aA6K3onW6e8c9yBUB0YLqWQTjm280z16-mOvLv0TiX_WrDOOoZqcRxNwrxULZTkwLn4L9jC0A1c8BVkF9CWXGtBr6cSoiknzUCfreiDXq3osxUNSq9W1szba_myi-j-Jq4aVuDDBcD1F8eARVcbMFl0oax2tMvhH_V_ADG7oJQ</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Gromadzka, Beata</creator><creator>Smietanka, Krzysztof</creator><creator>Dragun, Jakub</creator><creator>Minta, Zenon</creator><creator>Gora-Sochacka, Anna</creator><creator>Szewczyk, Boguslaw</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism</title><author>Gromadzka, Beata ; 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A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to electrophoresis. After silver staining, ssDNA band patterns characteristic for each genotype were observed. Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18957320</pmid><doi>10.1016/j.mcp.2008.09.003</doi><tpages>4</tpages></addata></record> |
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| ispartof | Molecular and cellular probes, 2008-10, Vol.22 (5), p.301-304 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Avian influenza Birds - virology DNA, Complementary - genetics Genome, Viral Genotype Humans Influenza A virus Influenza A virus - genetics Influenza in Birds - classification Influenza in Birds - diagnosis Influenza in Birds - genetics Phylogeny Polymorphism, Single-Stranded Conformational Reverse Transcriptase Polymerase Chain Reaction - methods Sequence amplification Single-stranded conformational polymorphism Temperature |
| title | Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism |
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