Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3)
We have generated rat monoclonal antibodies specific for the mouse eotaxin receptor, C‐C chemokine receptor 3 (CCR3). Several anti‐CCR3 mAbs proved to be useful for in vivo depletion of CCR3‐expressing cells and immunofluorescent staining. In vivo CCR3 mAbs of the IgG2b isotype substantially deplete...
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| Veröffentlicht in: | Journal of leukocyte biology 1999-06, Vol.65 (6), p.846-853 |
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| creator | Grimaldi, J. Christopher Yu, Nai‐Xuan Grunig, Gabrielle Seymour, Brian W. P. Cottrez, Françoise Robinson, Douglas S. Hosken, Nancy Ferlin, Walter G. Wu, Xiaoyan Soto, Hortensia O'Garra, Anne Howard, Maureen C. Coffman, Robert L. |
| description | We have generated rat monoclonal antibodies specific for the mouse eotaxin receptor, C‐C chemokine receptor 3 (CCR3). Several anti‐CCR3 mAbs proved to be useful for in vivo depletion of CCR3‐expressing cells and immunofluorescent staining. In vivo CCR3 mAbs of the IgG2b isotype substantially depleted blood eosinophil levels in Nippostrongyus brasiliensis‐infected mice. Repeated anti‐CCR3 mAb treatment in these mice significantly reduced tissue eosinophilia in the lung tissue and bronchoalveolar lavage fluid. Flow cytometry revealed that mCCR3 was expressed on eosinophils but not on stem cells, dendritic cells, or cells from the thymus, lymph node, or spleen of normal mice. Unlike human Th2 cells, mouse Th2 cells did not express detectable levels of CCR3 nor did they give a measurable response to eotaxin. None of the mAbs were antagonists or agonists of CCR3 calcium mobilization. To our knowledge, the antibodies described here are the first mAbs reported to be specific for mouse eosinophils and to be readily applicable for the detection, isolation, and in vivo depletion of eosinophils. J. Leukoc. Biol. 65: 846–853; 1999. |
| doi_str_mv | 10.1002/jlb.65.6.846 |
| format | Article |
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Christopher ; Yu, Nai‐Xuan ; Grunig, Gabrielle ; Seymour, Brian W. P. ; Cottrez, Françoise ; Robinson, Douglas S. ; Hosken, Nancy ; Ferlin, Walter G. ; Wu, Xiaoyan ; Soto, Hortensia ; O'Garra, Anne ; Howard, Maureen C. ; Coffman, Robert L.</creator><creatorcontrib>Grimaldi, J. Christopher ; Yu, Nai‐Xuan ; Grunig, Gabrielle ; Seymour, Brian W. P. ; Cottrez, Françoise ; Robinson, Douglas S. ; Hosken, Nancy ; Ferlin, Walter G. ; Wu, Xiaoyan ; Soto, Hortensia ; O'Garra, Anne ; Howard, Maureen C. ; Coffman, Robert L.</creatorcontrib><description>We have generated rat monoclonal antibodies specific for the mouse eotaxin receptor, C‐C chemokine receptor 3 (CCR3). Several anti‐CCR3 mAbs proved to be useful for in vivo depletion of CCR3‐expressing cells and immunofluorescent staining. In vivo CCR3 mAbs of the IgG2b isotype substantially depleted blood eosinophil levels in Nippostrongyus brasiliensis‐infected mice. Repeated anti‐CCR3 mAb treatment in these mice significantly reduced tissue eosinophilia in the lung tissue and bronchoalveolar lavage fluid. Flow cytometry revealed that mCCR3 was expressed on eosinophils but not on stem cells, dendritic cells, or cells from the thymus, lymph node, or spleen of normal mice. Unlike human Th2 cells, mouse Th2 cells did not express detectable levels of CCR3 nor did they give a measurable response to eotaxin. None of the mAbs were antagonists or agonists of CCR3 calcium mobilization. To our knowledge, the antibodies described here are the first mAbs reported to be specific for mouse eosinophils and to be readily applicable for the detection, isolation, and in vivo depletion of eosinophils. J. Leukoc. Biol. 65: 846–853; 1999.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1002/jlb.65.6.846</identifier><identifier>PMID: 10380909</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>3T3 Cells ; AIDS/HIV ; Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - pharmacology ; Antibody Formation ; Antibody Specificity ; Bronchoalveolar Lavage Fluid - parasitology ; Disease Models, Animal ; DNA, Complementary - biosynthesis ; Eosinophils - cytology ; Epitope Mapping ; FACS ; Humans ; Leukocyte Count - drug effects ; Lung - parasitology ; Mice ; Molecular Sequence Data ; Nippostrongylus ; Rats ; Receptors, CCR3 ; Receptors, Chemokine - genetics ; Receptors, Chemokine - immunology ; rodent ; Strongylida Infections ; Th1 ; Th2 ; Th2 Cells - immunology ; Tumor Cells, Cultured</subject><ispartof>Journal of leukocyte biology, 1999-06, Vol.65 (6), p.846-853</ispartof><rights>1999 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4946-3b831f71126945f9ef3898fd3a57978aef6547b1d92c16125f6d43c0c5fb433b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjlb.65.6.846$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjlb.65.6.846$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10380909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grimaldi, J. Christopher</creatorcontrib><creatorcontrib>Yu, Nai‐Xuan</creatorcontrib><creatorcontrib>Grunig, Gabrielle</creatorcontrib><creatorcontrib>Seymour, Brian W. P.</creatorcontrib><creatorcontrib>Cottrez, Françoise</creatorcontrib><creatorcontrib>Robinson, Douglas S.</creatorcontrib><creatorcontrib>Hosken, Nancy</creatorcontrib><creatorcontrib>Ferlin, Walter G.</creatorcontrib><creatorcontrib>Wu, Xiaoyan</creatorcontrib><creatorcontrib>Soto, Hortensia</creatorcontrib><creatorcontrib>O'Garra, Anne</creatorcontrib><creatorcontrib>Howard, Maureen C.</creatorcontrib><creatorcontrib>Coffman, Robert L.</creatorcontrib><title>Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3)</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>We have generated rat monoclonal antibodies specific for the mouse eotaxin receptor, C‐C chemokine receptor 3 (CCR3). Several anti‐CCR3 mAbs proved to be useful for in vivo depletion of CCR3‐expressing cells and immunofluorescent staining. In vivo CCR3 mAbs of the IgG2b isotype substantially depleted blood eosinophil levels in Nippostrongyus brasiliensis‐infected mice. Repeated anti‐CCR3 mAb treatment in these mice significantly reduced tissue eosinophilia in the lung tissue and bronchoalveolar lavage fluid. Flow cytometry revealed that mCCR3 was expressed on eosinophils but not on stem cells, dendritic cells, or cells from the thymus, lymph node, or spleen of normal mice. Unlike human Th2 cells, mouse Th2 cells did not express detectable levels of CCR3 nor did they give a measurable response to eotaxin. None of the mAbs were antagonists or agonists of CCR3 calcium mobilization. To our knowledge, the antibodies described here are the first mAbs reported to be specific for mouse eosinophils and to be readily applicable for the detection, isolation, and in vivo depletion of eosinophils. J. Leukoc. Biol. 65: 846–853; 1999.</description><subject>3T3 Cells</subject><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antibody Formation</subject><subject>Antibody Specificity</subject><subject>Bronchoalveolar Lavage Fluid - parasitology</subject><subject>Disease Models, Animal</subject><subject>DNA, Complementary - biosynthesis</subject><subject>Eosinophils - cytology</subject><subject>Epitope Mapping</subject><subject>FACS</subject><subject>Humans</subject><subject>Leukocyte Count - drug effects</subject><subject>Lung - parasitology</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nippostrongylus</subject><subject>Rats</subject><subject>Receptors, CCR3</subject><subject>Receptors, Chemokine - genetics</subject><subject>Receptors, Chemokine - immunology</subject><subject>rodent</subject><subject>Strongylida Infections</subject><subject>Th1</subject><subject>Th2</subject><subject>Th2 Cells - immunology</subject><subject>Tumor Cells, Cultured</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1TAUhoMoznV051qyURTsNWk-miy1M35xQRBdhzY9mWYmbWrSUubf26GDuNLVgcNznhfOi9BzSo6UkPLddWiPUhzlUXH5AB2oZqpgsmIP0YFUnBaCE3KGnuR8TQhhpSSP0RklTBFN9AHdXMAUYPZxxNFhiNmPcep9yNiPePAW8NynuFz12wS8ZLjDmnH2bew8ZJwnsN55i11MuC5qbHsY4o0fASewMM3bmuHXdf2dvXmKHrkmZHh2P8_Rz4-XP-rPxenbpy_1-1NhueayYK1i1FWUllJz4TQ4prRyHWtEpSvVgJOCVy3tdGmppKVwsuPMEitcyxlr2Tl6tXunFH8tkGcz-GwhhGaEuGQjtWJaKf5fkFallkJUG_h2B22KOSdwZkp-aNKtocTctWC2FowURpqthQ1_ce9d2gG6v-D97RtAd2D1AW7_KTNfTx_ILn253_T-ql99ApOHJoQtojTruv4J_w2opp3g</recordid><startdate>199906</startdate><enddate>199906</enddate><creator>Grimaldi, J. Christopher</creator><creator>Yu, Nai‐Xuan</creator><creator>Grunig, Gabrielle</creator><creator>Seymour, Brian W. P.</creator><creator>Cottrez, Françoise</creator><creator>Robinson, Douglas S.</creator><creator>Hosken, Nancy</creator><creator>Ferlin, Walter G.</creator><creator>Wu, Xiaoyan</creator><creator>Soto, Hortensia</creator><creator>O'Garra, Anne</creator><creator>Howard, Maureen C.</creator><creator>Coffman, Robert L.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199906</creationdate><title>Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3)</title><author>Grimaldi, J. Christopher ; Yu, Nai‐Xuan ; Grunig, Gabrielle ; Seymour, Brian W. P. ; Cottrez, Françoise ; Robinson, Douglas S. ; Hosken, Nancy ; Ferlin, Walter G. ; Wu, Xiaoyan ; Soto, Hortensia ; O'Garra, Anne ; Howard, Maureen C. ; Coffman, Robert L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4946-3b831f71126945f9ef3898fd3a57978aef6547b1d92c16125f6d43c0c5fb433b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>3T3 Cells</topic><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antibody Formation</topic><topic>Antibody Specificity</topic><topic>Bronchoalveolar Lavage Fluid - parasitology</topic><topic>Disease Models, Animal</topic><topic>DNA, Complementary - biosynthesis</topic><topic>Eosinophils - cytology</topic><topic>Epitope Mapping</topic><topic>FACS</topic><topic>Humans</topic><topic>Leukocyte Count - drug effects</topic><topic>Lung - parasitology</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Nippostrongylus</topic><topic>Rats</topic><topic>Receptors, CCR3</topic><topic>Receptors, Chemokine - genetics</topic><topic>Receptors, Chemokine - immunology</topic><topic>rodent</topic><topic>Strongylida Infections</topic><topic>Th1</topic><topic>Th2</topic><topic>Th2 Cells - immunology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grimaldi, J. Christopher</creatorcontrib><creatorcontrib>Yu, Nai‐Xuan</creatorcontrib><creatorcontrib>Grunig, Gabrielle</creatorcontrib><creatorcontrib>Seymour, Brian W. 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Christopher</au><au>Yu, Nai‐Xuan</au><au>Grunig, Gabrielle</au><au>Seymour, Brian W. P.</au><au>Cottrez, Françoise</au><au>Robinson, Douglas S.</au><au>Hosken, Nancy</au><au>Ferlin, Walter G.</au><au>Wu, Xiaoyan</au><au>Soto, Hortensia</au><au>O'Garra, Anne</au><au>Howard, Maureen C.</au><au>Coffman, Robert L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3)</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>1999-06</date><risdate>1999</risdate><volume>65</volume><issue>6</issue><spage>846</spage><epage>853</epage><pages>846-853</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>We have generated rat monoclonal antibodies specific for the mouse eotaxin receptor, C‐C chemokine receptor 3 (CCR3). Several anti‐CCR3 mAbs proved to be useful for in vivo depletion of CCR3‐expressing cells and immunofluorescent staining. In vivo CCR3 mAbs of the IgG2b isotype substantially depleted blood eosinophil levels in Nippostrongyus brasiliensis‐infected mice. Repeated anti‐CCR3 mAb treatment in these mice significantly reduced tissue eosinophilia in the lung tissue and bronchoalveolar lavage fluid. Flow cytometry revealed that mCCR3 was expressed on eosinophils but not on stem cells, dendritic cells, or cells from the thymus, lymph node, or spleen of normal mice. Unlike human Th2 cells, mouse Th2 cells did not express detectable levels of CCR3 nor did they give a measurable response to eotaxin. None of the mAbs were antagonists or agonists of CCR3 calcium mobilization. To our knowledge, the antibodies described here are the first mAbs reported to be specific for mouse eosinophils and to be readily applicable for the detection, isolation, and in vivo depletion of eosinophils. J. Leukoc. Biol. 65: 846–853; 1999.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>10380909</pmid><doi>10.1002/jlb.65.6.846</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of leukocyte biology, 1999-06, Vol.65 (6), p.846-853 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 3T3 Cells AIDS/HIV Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology Antibody Formation Antibody Specificity Bronchoalveolar Lavage Fluid - parasitology Disease Models, Animal DNA, Complementary - biosynthesis Eosinophils - cytology Epitope Mapping FACS Humans Leukocyte Count - drug effects Lung - parasitology Mice Molecular Sequence Data Nippostrongylus Rats Receptors, CCR3 Receptors, Chemokine - genetics Receptors, Chemokine - immunology rodent Strongylida Infections Th1 Th2 Th2 Cells - immunology Tumor Cells, Cultured |
| title | Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3) |
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