Endothelial monocyte-activating polypeptide-2 is increased in pregnancy but is not further increased in preeclampsia
Endothelial monocyte-activating polypeptide-2 (EMAP-2) is a novel protein that demonstrates potent proinflammatory activity in vivo and in vitro. The purpose of this study was to investigate the expression of EMAP-2 in normal pregnancy and in pregnancies complicated with preeclampsia and to test the...
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| Veröffentlicht in: | Journal of the Society for Gynecologic Investigation 1999-05, Vol.6 (3), p.142-146 |
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| container_title | Journal of the Society for Gynecologic Investigation |
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| creator | WELLINGS, R. P LASH, G. E MURRAY, J. C TAS, M WARD, W TREW, A. J BAKER, P. N |
| description | Endothelial monocyte-activating polypeptide-2 (EMAP-2) is a novel protein that demonstrates potent proinflammatory activity in vivo and in vitro. The purpose of this study was to investigate the expression of EMAP-2 in normal pregnancy and in pregnancies complicated with preeclampsia and to test the hypothesis that EMAP-2 is a causative agent of the endothelial activation of preeclampsia.
Expression of EMAP-2 in the placenta was investigated using reverse transcriptase-polymerase chain reaction to detect mRNA, and immunohistochemistry with an EMAP-2-specific polyclonal antiserum was carried out to detect protein. Levels of circulating EMAP-2 protein were measured in the blood of nonpregnant, normal pregnant, and preeclamptic individuals using a specific enzyme-linked immunoabsorbent assay and the molecular forms present assessed by Western blotting.
EMAP-2 is transcribed and translated by the placenta troughout pregnancy and in preeclampsia and can be detected in the plasma of nonpregnant and pregnant individuals. Levels of circulating EMAP-2 antigen are raised in pregnancy compared with nonpregnant controls; however, levels in patients with preeclampsia are identical to those in normal pregnant individuals.
While circulating levels of EMAP-2 are increased in pregnancy, there is no evidence that EMAP-2 is involved directly in the pathogenesis of preeclampsia. |
| doi_str_mv | 10.1016/S1071-5576(99)00011-8 |
| format | Article |
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Expression of EMAP-2 in the placenta was investigated using reverse transcriptase-polymerase chain reaction to detect mRNA, and immunohistochemistry with an EMAP-2-specific polyclonal antiserum was carried out to detect protein. Levels of circulating EMAP-2 protein were measured in the blood of nonpregnant, normal pregnant, and preeclamptic individuals using a specific enzyme-linked immunoabsorbent assay and the molecular forms present assessed by Western blotting.
EMAP-2 is transcribed and translated by the placenta troughout pregnancy and in preeclampsia and can be detected in the plasma of nonpregnant and pregnant individuals. Levels of circulating EMAP-2 antigen are raised in pregnancy compared with nonpregnant controls; however, levels in patients with preeclampsia are identical to those in normal pregnant individuals.
While circulating levels of EMAP-2 are increased in pregnancy, there is no evidence that EMAP-2 is involved directly in the pathogenesis of preeclampsia.</description><identifier>ISSN: 1071-5576</identifier><identifier>EISSN: 1556-7117</identifier><identifier>DOI: 10.1016/S1071-5576(99)00011-8</identifier><identifier>PMID: 10376270</identifier><language>eng</language><publisher>New York, NY: Elsevier</publisher><subject>Adult ; Biological and medical sciences ; Blotting, Western ; Cytokines ; Diseases of mother, fetus and pregnancy ; Enzyme-Linked Immunosorbent Assay ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Medical sciences ; Neoplasm Proteins - blood ; Neoplasm Proteins - metabolism ; Placenta - metabolism ; Pre-Eclampsia - blood ; Pre-Eclampsia - metabolism ; Pregnancy ; Pregnancy Trimesters ; Pregnancy. Fetus. Placenta ; Reverse Transcriptase Polymerase Chain Reaction ; RNA-Binding Proteins - blood ; RNA-Binding Proteins - metabolism</subject><ispartof>Journal of the Society for Gynecologic Investigation, 1999-05, Vol.6 (3), p.142-146</ispartof><rights>1999 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-8d6f290f4f654054afe524e83db943079f3d4292a3a4ba8bf924938f34ac70223</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1800673$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10376270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WELLINGS, R. P</creatorcontrib><creatorcontrib>LASH, G. E</creatorcontrib><creatorcontrib>MURRAY, J. C</creatorcontrib><creatorcontrib>TAS, M</creatorcontrib><creatorcontrib>WARD, W</creatorcontrib><creatorcontrib>TREW, A. J</creatorcontrib><creatorcontrib>BAKER, P. N</creatorcontrib><title>Endothelial monocyte-activating polypeptide-2 is increased in pregnancy but is not further increased in preeclampsia</title><title>Journal of the Society for Gynecologic Investigation</title><addtitle>J Soc Gynecol Investig</addtitle><description>Endothelial monocyte-activating polypeptide-2 (EMAP-2) is a novel protein that demonstrates potent proinflammatory activity in vivo and in vitro. The purpose of this study was to investigate the expression of EMAP-2 in normal pregnancy and in pregnancies complicated with preeclampsia and to test the hypothesis that EMAP-2 is a causative agent of the endothelial activation of preeclampsia.
Expression of EMAP-2 in the placenta was investigated using reverse transcriptase-polymerase chain reaction to detect mRNA, and immunohistochemistry with an EMAP-2-specific polyclonal antiserum was carried out to detect protein. Levels of circulating EMAP-2 protein were measured in the blood of nonpregnant, normal pregnant, and preeclamptic individuals using a specific enzyme-linked immunoabsorbent assay and the molecular forms present assessed by Western blotting.
EMAP-2 is transcribed and translated by the placenta troughout pregnancy and in preeclampsia and can be detected in the plasma of nonpregnant and pregnant individuals. Levels of circulating EMAP-2 antigen are raised in pregnancy compared with nonpregnant controls; however, levels in patients with preeclampsia are identical to those in normal pregnant individuals.
While circulating levels of EMAP-2 are increased in pregnancy, there is no evidence that EMAP-2 is involved directly in the pathogenesis of preeclampsia.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cytokines</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Neoplasm Proteins - blood</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Placenta - metabolism</subject><subject>Pre-Eclampsia - blood</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy Trimesters</subject><subject>Pregnancy. Fetus. 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Placenta</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA-Binding Proteins - blood</topic><topic>RNA-Binding Proteins - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>WELLINGS, R. P</creatorcontrib><creatorcontrib>LASH, G. E</creatorcontrib><creatorcontrib>MURRAY, J. C</creatorcontrib><creatorcontrib>TAS, M</creatorcontrib><creatorcontrib>WARD, W</creatorcontrib><creatorcontrib>TREW, A. J</creatorcontrib><creatorcontrib>BAKER, P. 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N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelial monocyte-activating polypeptide-2 is increased in pregnancy but is not further increased in preeclampsia</atitle><jtitle>Journal of the Society for Gynecologic Investigation</jtitle><addtitle>J Soc Gynecol Investig</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>6</volume><issue>3</issue><spage>142</spage><epage>146</epage><pages>142-146</pages><issn>1071-5576</issn><eissn>1556-7117</eissn><abstract>Endothelial monocyte-activating polypeptide-2 (EMAP-2) is a novel protein that demonstrates potent proinflammatory activity in vivo and in vitro. The purpose of this study was to investigate the expression of EMAP-2 in normal pregnancy and in pregnancies complicated with preeclampsia and to test the hypothesis that EMAP-2 is a causative agent of the endothelial activation of preeclampsia.
Expression of EMAP-2 in the placenta was investigated using reverse transcriptase-polymerase chain reaction to detect mRNA, and immunohistochemistry with an EMAP-2-specific polyclonal antiserum was carried out to detect protein. Levels of circulating EMAP-2 protein were measured in the blood of nonpregnant, normal pregnant, and preeclamptic individuals using a specific enzyme-linked immunoabsorbent assay and the molecular forms present assessed by Western blotting.
EMAP-2 is transcribed and translated by the placenta troughout pregnancy and in preeclampsia and can be detected in the plasma of nonpregnant and pregnant individuals. Levels of circulating EMAP-2 antigen are raised in pregnancy compared with nonpregnant controls; however, levels in patients with preeclampsia are identical to those in normal pregnant individuals.
While circulating levels of EMAP-2 are increased in pregnancy, there is no evidence that EMAP-2 is involved directly in the pathogenesis of preeclampsia.</abstract><cop>New York, NY</cop><pub>Elsevier</pub><pmid>10376270</pmid><doi>10.1016/S1071-5576(99)00011-8</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of the Society for Gynecologic Investigation, 1999-05, Vol.6 (3), p.142-146 |
| issn | 1071-5576 1556-7117 |
| language | eng |
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| source | MEDLINE; SAGE Complete; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Blotting, Western Cytokines Diseases of mother, fetus and pregnancy Enzyme-Linked Immunosorbent Assay Female Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Medical sciences Neoplasm Proteins - blood Neoplasm Proteins - metabolism Placenta - metabolism Pre-Eclampsia - blood Pre-Eclampsia - metabolism Pregnancy Pregnancy Trimesters Pregnancy. Fetus. Placenta Reverse Transcriptase Polymerase Chain Reaction RNA-Binding Proteins - blood RNA-Binding Proteins - metabolism |
| title | Endothelial monocyte-activating polypeptide-2 is increased in pregnancy but is not further increased in preeclampsia |
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