Suppression of the Heterotrimeric G Protein Causes Abnormal Morphology, including Dwarfism, in Rice
Transgenic rice containing an antisense cDNA for the α subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the α subunit was abundant in the internod...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-06, Vol.96 (13), p.7575-7580 |
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| creator | Fujisawa, Yukiko Kato, Teruhisa Ohki, Shizuka Ishikawa, Atsushi Kitano, Hidemi Sasaki, Takuji Asahi, Tadashi Iwasaki, Yukimoto |
| description | Transgenic rice containing an antisense cDNA for the α subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the α subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the α -subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the α subunit by antisense technology. Analysis of the cDNAs for the α subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the α -subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice. |
| doi_str_mv | 10.1073/pnas.96.13.7575 |
| format | Article |
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In normal rice, the mRNA for the α subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the α -subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the α subunit by antisense technology. Analysis of the cDNAs for the α subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the α -subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.96.13.7575</identifier><identifier>PMID: 10377457</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Alleles ; Base Sequence ; Biological Sciences ; Botany ; Complementary DNA ; Deoxyribonucleic acid ; DNA ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant ; Genetic engineering ; GTP-Binding Proteins - genetics ; Internodes ; Messenger RNA ; Molecular Sequence Data ; Oryza - anatomy & histology ; Oryza - genetics ; Oryza sativa ; Phenotypes ; Plant Proteins - genetics ; Plants ; Plants, Genetically Modified ; Proteins ; Ribonucleic acid ; Rice ; RNA ; Seeds ; Transgenic plants</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1999-06, Vol.96 (13), p.7575-7580</ispartof><rights>Copyright 1993-1999 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Jun 22, 1999</rights><rights>Copyright © 1999, The National Academy of Sciences 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c585t-1a4fe12d9fe515c37c804fcd8b615f908d157438dad74588ccd94351f6720c6a3</citedby><cites>FETCH-LOGICAL-c585t-1a4fe12d9fe515c37c804fcd8b615f908d157438dad74588ccd94351f6720c6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/96/13.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48101$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48101$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10377457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujisawa, Yukiko</creatorcontrib><creatorcontrib>Kato, Teruhisa</creatorcontrib><creatorcontrib>Ohki, Shizuka</creatorcontrib><creatorcontrib>Ishikawa, Atsushi</creatorcontrib><creatorcontrib>Kitano, Hidemi</creatorcontrib><creatorcontrib>Sasaki, Takuji</creatorcontrib><creatorcontrib>Asahi, Tadashi</creatorcontrib><creatorcontrib>Iwasaki, Yukimoto</creatorcontrib><title>Suppression of the Heterotrimeric G Protein Causes Abnormal Morphology, including Dwarfism, in Rice</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Transgenic rice containing an antisense cDNA for the α subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the α subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the α -subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the α subunit by antisense technology. Analysis of the cDNAs for the α subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the α -subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice.</description><subject>Alleles</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Botany</subject><subject>Complementary DNA</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genetic engineering</subject><subject>GTP-Binding Proteins - genetics</subject><subject>Internodes</subject><subject>Messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Oryza - anatomy & histology</subject><subject>Oryza - genetics</subject><subject>Oryza sativa</subject><subject>Phenotypes</subject><subject>Plant Proteins - genetics</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>Rice</subject><subject>RNA</subject><subject>Seeds</subject><subject>Transgenic plants</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxSMEokvhjMQBWRzgQrb-jG2JS7VAW6kIxMfZ8jr2rleJndoJ0P8eR9tWWw5wGnnm90bz_KrqOYJLBDk5GYLOS9ksEVlyxtmDaoGgRHVDJXxYLSDEvBYU06PqSc47CKFkAj6ujhAknFPGF5X5Ng1Dsjn7GEB0YNxacG5Hm-KYfG-TN-AMfCkv6wNY6SnbDE7XIaZed-BTTMM2dnFz_Rb4YLqp9WED3v_Syfnczz3w1Rv7tHrkdJfts5t6XP34-OH76ry-_Hx2sTq9rA0TbKyRps4i3EpnGWKGcCMgdaYV6wYxJ6FoEeOUiFa35XYhjGklJQy5hmNoGk2Oq3f7vcO07m1rbBiT7tRQjOh0raL26v4k-K3axJ8KY4RFkb--kad4Ndk8qt5nY7tOBxunrBopCBeI_RdEHDcMElzAV3-BuzilUP5AYYiIkBDSAp3sIZNizsm6u4MRVHPIag5ZyUYhouaQi-Lloc8Dfp_qATArb8f3Nrz5J6Dc1HWj_T0W8sWe3OUxpjuUClQM_AH_LsUH</recordid><startdate>19990622</startdate><enddate>19990622</enddate><creator>Fujisawa, Yukiko</creator><creator>Kato, Teruhisa</creator><creator>Ohki, Shizuka</creator><creator>Ishikawa, Atsushi</creator><creator>Kitano, Hidemi</creator><creator>Sasaki, Takuji</creator><creator>Asahi, Tadashi</creator><creator>Iwasaki, Yukimoto</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990622</creationdate><title>Suppression of the Heterotrimeric G Protein Causes Abnormal Morphology, including Dwarfism, in Rice</title><author>Fujisawa, Yukiko ; Kato, Teruhisa ; Ohki, Shizuka ; Ishikawa, Atsushi ; Kitano, Hidemi ; Sasaki, Takuji ; Asahi, Tadashi ; Iwasaki, Yukimoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-1a4fe12d9fe515c37c804fcd8b615f908d157438dad74588ccd94351f6720c6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Alleles</topic><topic>Base Sequence</topic><topic>Biological Sciences</topic><topic>Botany</topic><topic>Complementary DNA</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genetic engineering</topic><topic>GTP-Binding Proteins - genetics</topic><topic>Internodes</topic><topic>Messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Oryza - anatomy & histology</topic><topic>Oryza - genetics</topic><topic>Oryza sativa</topic><topic>Phenotypes</topic><topic>Plant Proteins - genetics</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>Rice</topic><topic>RNA</topic><topic>Seeds</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujisawa, Yukiko</creatorcontrib><creatorcontrib>Kato, Teruhisa</creatorcontrib><creatorcontrib>Ohki, Shizuka</creatorcontrib><creatorcontrib>Ishikawa, Atsushi</creatorcontrib><creatorcontrib>Kitano, Hidemi</creatorcontrib><creatorcontrib>Sasaki, Takuji</creatorcontrib><creatorcontrib>Asahi, Tadashi</creatorcontrib><creatorcontrib>Iwasaki, Yukimoto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujisawa, Yukiko</au><au>Kato, Teruhisa</au><au>Ohki, Shizuka</au><au>Ishikawa, Atsushi</au><au>Kitano, Hidemi</au><au>Sasaki, Takuji</au><au>Asahi, Tadashi</au><au>Iwasaki, Yukimoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of the Heterotrimeric G Protein Causes Abnormal Morphology, including Dwarfism, in Rice</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1999-06-22</date><risdate>1999</risdate><volume>96</volume><issue>13</issue><spage>7575</spage><epage>7580</epage><pages>7575-7580</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Transgenic rice containing an antisense cDNA for the α subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the α subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the α -subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the α subunit by antisense technology. Analysis of the cDNAs for the α subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the α -subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>10377457</pmid><doi>10.1073/pnas.96.13.7575</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alleles Base Sequence Biological Sciences Botany Complementary DNA Deoxyribonucleic acid DNA Gene Expression Regulation, Plant Genes Genes, Plant Genetic engineering GTP-Binding Proteins - genetics Internodes Messenger RNA Molecular Sequence Data Oryza - anatomy & histology Oryza - genetics Oryza sativa Phenotypes Plant Proteins - genetics Plants Plants, Genetically Modified Proteins Ribonucleic acid Rice RNA Seeds Transgenic plants |
| title | Suppression of the Heterotrimeric G Protein Causes Abnormal Morphology, including Dwarfism, in Rice |
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