Ligand regulation of juvenile hormone binding protein mRNA in mutant Manduca sexta
Insect hemolymph juvenile hormone binding protein (hJHBP) regulates peripheral titers of its ligands, the juvenile hormones. In larvae of the black ( bl) strain of the tobacco hornworm, Manduca sexta, treatment with small doses of juvenile hormone I (JH I) can also regulate titers of hJHBP. To furth...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1999-03, Vol.149 (1), p.61-69 |
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| creator | Orth, Anthony P. Lan, Que Goodman, Walter G. |
| description | Insect hemolymph juvenile hormone binding protein (hJHBP) regulates peripheral titers of its ligands, the juvenile hormones. In larvae of the
black (
bl) strain of the tobacco hornworm,
Manduca sexta, treatment with small doses of juvenile hormone I (JH I) can also regulate titers of hJHBP. To further investigate this regulation, responsiveness of hJHBP mRNA expression to JH I was characterized in vivo. RNA analyzes revealed that transcript levels in fat body, the site of hJHBP synthesis, increased fivefold within several hours of treatment with physiological doses of hormone and remained elevated for approximately 16 h. Sensitivity to JH treatment was found to vary temporally. To ensure transcript identity, a wild-type cDNA clone and a
bl RT-PCR fragment were sequenced and found to be 99% homologous. Together, these results suggest that JH participates in regulating expression of its transport protein in
bl larvae by modifying the in vivo abundance of hJHBP’s mRNA transcript. |
| doi_str_mv | 10.1016/S0303-7207(98)00258-5 |
| format | Article |
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black (
bl) strain of the tobacco hornworm,
Manduca sexta, treatment with small doses of juvenile hormone I (JH I) can also regulate titers of hJHBP. To further investigate this regulation, responsiveness of hJHBP mRNA expression to JH I was characterized in vivo. RNA analyzes revealed that transcript levels in fat body, the site of hJHBP synthesis, increased fivefold within several hours of treatment with physiological doses of hormone and remained elevated for approximately 16 h. Sensitivity to JH treatment was found to vary temporally. To ensure transcript identity, a wild-type cDNA clone and a
bl RT-PCR fragment were sequenced and found to be 99% homologous. Together, these results suggest that JH participates in regulating expression of its transport protein in
bl larvae by modifying the in vivo abundance of hJHBP’s mRNA transcript.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/S0303-7207(98)00258-5</identifier><identifier>PMID: 10375018</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Black mutant ; Carrier Proteins - genetics ; Cloning, Molecular ; Consensus Sequence ; DNA, Complementary - genetics ; Ecdysteroids ; Fat Body - metabolism ; Hemolymph juvenile hormone binding protein ; Insect Hormones - metabolism ; Insect Proteins ; Juvenile hormone ; Juvenile Hormones - metabolism ; Larva - metabolism ; Ligands ; Manduca - genetics ; Manduca - growth & development ; Manduca - metabolism ; Manduca sexta ; Molecular Sequence Data ; mRNA ; Mutation ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Steroids - metabolism</subject><ispartof>Molecular and cellular endocrinology, 1999-03, Vol.149 (1), p.61-69</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-da3c37f2b7cf34c93585b879f2702a75eff8768e109de07f77348460959f77f23</citedby><cites>FETCH-LOGICAL-c361t-da3c37f2b7cf34c93585b879f2702a75eff8768e109de07f77348460959f77f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0303-7207(98)00258-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10375018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orth, Anthony P.</creatorcontrib><creatorcontrib>Lan, Que</creatorcontrib><creatorcontrib>Goodman, Walter G.</creatorcontrib><title>Ligand regulation of juvenile hormone binding protein mRNA in mutant Manduca sexta</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Insect hemolymph juvenile hormone binding protein (hJHBP) regulates peripheral titers of its ligands, the juvenile hormones. In larvae of the
black (
bl) strain of the tobacco hornworm,
Manduca sexta, treatment with small doses of juvenile hormone I (JH I) can also regulate titers of hJHBP. To further investigate this regulation, responsiveness of hJHBP mRNA expression to JH I was characterized in vivo. RNA analyzes revealed that transcript levels in fat body, the site of hJHBP synthesis, increased fivefold within several hours of treatment with physiological doses of hormone and remained elevated for approximately 16 h. Sensitivity to JH treatment was found to vary temporally. To ensure transcript identity, a wild-type cDNA clone and a
bl RT-PCR fragment were sequenced and found to be 99% homologous. Together, these results suggest that JH participates in regulating expression of its transport protein in
bl larvae by modifying the in vivo abundance of hJHBP’s mRNA transcript.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Black mutant</subject><subject>Carrier Proteins - genetics</subject><subject>Cloning, Molecular</subject><subject>Consensus Sequence</subject><subject>DNA, Complementary - genetics</subject><subject>Ecdysteroids</subject><subject>Fat Body - metabolism</subject><subject>Hemolymph juvenile hormone binding protein</subject><subject>Insect Hormones - metabolism</subject><subject>Insect Proteins</subject><subject>Juvenile hormone</subject><subject>Juvenile Hormones - metabolism</subject><subject>Larva - metabolism</subject><subject>Ligands</subject><subject>Manduca - genetics</subject><subject>Manduca - growth & development</subject><subject>Manduca - metabolism</subject><subject>Manduca sexta</subject><subject>Molecular Sequence Data</subject><subject>mRNA</subject><subject>Mutation</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Steroids - metabolism</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PGzEQhi1UBCH0J4B8qsphYbyO1_YJRVH5kEKRaHu2HO84ONr1gr2L6L9nQxDqraeZw_POx0PICYNzBqy6-AUceCFLkN-1OgMohSrEHpkwJctCgZBfyOQTOSRHOW8AQIpSHZBDBlwKYGpCHpZhbWNNE66Hxvahi7TzdDO8YAwN0scutV1EugqxDnFNn1LXY4i0ffg5p9s69Db29G4cMThLM7729pjse9tk_PpRp-TP1Y_fi5tieX99u5gvC8cr1he15Y5LX66k83zmNBdKrJTUvpRQWinQeyUrhQx0jSC9lHymZhVoocfel3xKvu3mjkc9D5h704bssGlsxG7IptKKV1qqERQ70KUu54TePKXQ2vTXMDBbmeZdptmaMlqZd5lGjLnTjwXDqsX6n9TO3ghc7gAc33wJmEx2AaPDOiR0vam78J8VbzZig3Q</recordid><startdate>19990325</startdate><enddate>19990325</enddate><creator>Orth, Anthony P.</creator><creator>Lan, Que</creator><creator>Goodman, Walter G.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990325</creationdate><title>Ligand regulation of juvenile hormone binding protein mRNA in mutant Manduca sexta</title><author>Orth, Anthony P. ; Lan, Que ; Goodman, Walter G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-da3c37f2b7cf34c93585b879f2702a75eff8768e109de07f77348460959f77f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Black mutant</topic><topic>Carrier Proteins - genetics</topic><topic>Cloning, Molecular</topic><topic>Consensus Sequence</topic><topic>DNA, Complementary - genetics</topic><topic>Ecdysteroids</topic><topic>Fat Body - metabolism</topic><topic>Hemolymph juvenile hormone binding protein</topic><topic>Insect Hormones - metabolism</topic><topic>Insect Proteins</topic><topic>Juvenile hormone</topic><topic>Juvenile Hormones - metabolism</topic><topic>Larva - metabolism</topic><topic>Ligands</topic><topic>Manduca - genetics</topic><topic>Manduca - growth & development</topic><topic>Manduca - metabolism</topic><topic>Manduca sexta</topic><topic>Molecular Sequence Data</topic><topic>mRNA</topic><topic>Mutation</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Steroids - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orth, Anthony P.</creatorcontrib><creatorcontrib>Lan, Que</creatorcontrib><creatorcontrib>Goodman, Walter G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orth, Anthony P.</au><au>Lan, Que</au><au>Goodman, Walter G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ligand regulation of juvenile hormone binding protein mRNA in mutant Manduca sexta</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1999-03-25</date><risdate>1999</risdate><volume>149</volume><issue>1</issue><spage>61</spage><epage>69</epage><pages>61-69</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Insect hemolymph juvenile hormone binding protein (hJHBP) regulates peripheral titers of its ligands, the juvenile hormones. In larvae of the
black (
bl) strain of the tobacco hornworm,
Manduca sexta, treatment with small doses of juvenile hormone I (JH I) can also regulate titers of hJHBP. To further investigate this regulation, responsiveness of hJHBP mRNA expression to JH I was characterized in vivo. RNA analyzes revealed that transcript levels in fat body, the site of hJHBP synthesis, increased fivefold within several hours of treatment with physiological doses of hormone and remained elevated for approximately 16 h. Sensitivity to JH treatment was found to vary temporally. To ensure transcript identity, a wild-type cDNA clone and a
bl RT-PCR fragment were sequenced and found to be 99% homologous. Together, these results suggest that JH participates in regulating expression of its transport protein in
bl larvae by modifying the in vivo abundance of hJHBP’s mRNA transcript.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>10375018</pmid><doi>10.1016/S0303-7207(98)00258-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular and cellular endocrinology, 1999-03, Vol.149 (1), p.61-69 |
| issn | 0303-7207 1872-8057 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Animals Black mutant Carrier Proteins - genetics Cloning, Molecular Consensus Sequence DNA, Complementary - genetics Ecdysteroids Fat Body - metabolism Hemolymph juvenile hormone binding protein Insect Hormones - metabolism Insect Proteins Juvenile hormone Juvenile Hormones - metabolism Larva - metabolism Ligands Manduca - genetics Manduca - growth & development Manduca - metabolism Manduca sexta Molecular Sequence Data mRNA Mutation RNA, Messenger - genetics RNA, Messenger - metabolism Steroids - metabolism |
| title | Ligand regulation of juvenile hormone binding protein mRNA in mutant Manduca sexta |
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