Role of STAT5 in Interferon-α Signal Transduction in Ba/F3 Cells
In interferon-α (IFN-α) signalling, the essential role of the transcription factors STAT1 and STAT2 is well established. In contrast, the involvement of other STAT proteins, including STAT5, is much less well understood. Here we show that, in IFN-α-responsive Ba/F3 cells, this cytokine stimulates th...
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| Veröffentlicht in: | Cellular signalling 1999-05, Vol.11 (5), p.331-335 |
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| creator | Jaster, Robert Tschirch, Edda Bittorf, Thomas Brock, Josef |
| description | In interferon-α (IFN-α) signalling, the essential role of the transcription factors STAT1 and STAT2 is well established. In contrast, the involvement of other STAT proteins, including STAT5, is much less well understood. Here we show that, in IFN-α-responsive Ba/F3 cells, this cytokine stimulates the DNA-binding of STAT5A and B but that IL-3 is a much more potent activator of both STAT5 isoforms. A stably expressed dominant-negative mutant of JAK2 suppressed the IL-3- but not the IFN-α-dependent DNA binding of STAT5, suggesting independent mechanisms of its activation. Northern blots revealed that IL-3 strongly induced the expression of two STAT5-regulated genes,
pim-1 and
oncostatin-M, whereas IFN-α had a weak stimulatory effect on
pim-1 expression only. In summary, our results suggest that, despite the capability of IFN-α to stimulate DNA binding of STAT5, this transcription factor does not play a pivotal role in IFN-α signalling in Ba/F3 cells. |
| doi_str_mv | 10.1016/S0898-6568(98)00049-7 |
| format | Article |
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pim-1 and
oncostatin-M, whereas IFN-α had a weak stimulatory effect on
pim-1 expression only. In summary, our results suggest that, despite the capability of IFN-α to stimulate DNA binding of STAT5, this transcription factor does not play a pivotal role in IFN-α signalling in Ba/F3 cells.</description><identifier>ISSN: 0898-6568</identifier><identifier>EISSN: 1873-3913</identifier><identifier>DOI: 10.1016/S0898-6568(98)00049-7</identifier><identifier>PMID: 10376805</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; B-Lymphocytes - cytology ; B-Lymphocytes - drug effects ; B-Lymphocytes - metabolism ; Ba/F3 cells ; Cell Line ; DNA - metabolism ; DNA-Binding Proteins - metabolism ; DNA-Binding Proteins - physiology ; Interferon-alpha - metabolism ; Interferon-α ; Interleukin-3 - metabolism ; Interleukin-3 - pharmacology ; Janus Kinase 2 ; Mice ; Milk Proteins ; Protein Binding ; Protein-Tyrosine Kinases - metabolism ; Proto-Oncogene Proteins ; Signal Transduction ; STAT5 ; STAT5 Transcription Factor ; Trans-Activators - metabolism ; Trans-Activators - physiology</subject><ispartof>Cellular signalling, 1999-05, Vol.11 (5), p.331-335</ispartof><rights>1999 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-c4a1990ab3113d3cda52f84ef457fbb04637c5d16de48a98b873c86006d7972b3</citedby><cites>FETCH-LOGICAL-c361t-c4a1990ab3113d3cda52f84ef457fbb04637c5d16de48a98b873c86006d7972b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0898-6568(98)00049-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10376805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jaster, Robert</creatorcontrib><creatorcontrib>Tschirch, Edda</creatorcontrib><creatorcontrib>Bittorf, Thomas</creatorcontrib><creatorcontrib>Brock, Josef</creatorcontrib><title>Role of STAT5 in Interferon-α Signal Transduction in Ba/F3 Cells</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>In interferon-α (IFN-α) signalling, the essential role of the transcription factors STAT1 and STAT2 is well established. In contrast, the involvement of other STAT proteins, including STAT5, is much less well understood. Here we show that, in IFN-α-responsive Ba/F3 cells, this cytokine stimulates the DNA-binding of STAT5A and B but that IL-3 is a much more potent activator of both STAT5 isoforms. A stably expressed dominant-negative mutant of JAK2 suppressed the IL-3- but not the IFN-α-dependent DNA binding of STAT5, suggesting independent mechanisms of its activation. Northern blots revealed that IL-3 strongly induced the expression of two STAT5-regulated genes,
pim-1 and
oncostatin-M, whereas IFN-α had a weak stimulatory effect on
pim-1 expression only. In summary, our results suggest that, despite the capability of IFN-α to stimulate DNA binding of STAT5, this transcription factor does not play a pivotal role in IFN-α signalling in Ba/F3 cells.</description><subject>Animals</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - metabolism</subject><subject>Ba/F3 cells</subject><subject>Cell Line</subject><subject>DNA - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Interferon-alpha - metabolism</subject><subject>Interferon-α</subject><subject>Interleukin-3 - metabolism</subject><subject>Interleukin-3 - pharmacology</subject><subject>Janus Kinase 2</subject><subject>Mice</subject><subject>Milk Proteins</subject><subject>Protein Binding</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins</subject><subject>Signal Transduction</subject><subject>STAT5</subject><subject>STAT5 Transcription Factor</subject><subject>Trans-Activators - metabolism</subject><subject>Trans-Activators - physiology</subject><issn>0898-6568</issn><issn>1873-3913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN1KwzAYhoMobk4vQemR6EFdsjR_RzKH08FAcPU4pEkqka6ZSSt4Wd6I12S3DvHMo_fk-X7eB4BzBG8QRHS8glzwlBLKrwS_hhBmImUHYIg4wykWCB-C4S8yACcxvkGICKSTYzBAEDPKIRmC6bOvbOLLZJVPc5K4OlnUjQ2lDb5Ov7-SlXutVZXkQdXRtLpxvt5Cd2o8x8nMVlU8BUelqqI92-cIvMzv89ljunx6WMymy1RjippUZwoJAVWBEcIGa6PIpOSZLTPCyqKAGcVME4OosRlXghddD80phNQwwSYFHoHLfu8m-PfWxkauXdTdB6q2vo2SCo5xxlAHkh7UwccYbCk3wa1V-JQIyq07uXMnt2Jklzt3knVzF_sDbbG25s9UL6sDbnvAdjU_nA0yamdrbY0LVjfSePfPiR_1zXya</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Jaster, Robert</creator><creator>Tschirch, Edda</creator><creator>Bittorf, Thomas</creator><creator>Brock, Josef</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990501</creationdate><title>Role of STAT5 in Interferon-α Signal Transduction in Ba/F3 Cells</title><author>Jaster, Robert ; Tschirch, Edda ; Bittorf, Thomas ; Brock, Josef</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-c4a1990ab3113d3cda52f84ef457fbb04637c5d16de48a98b873c86006d7972b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - drug effects</topic><topic>B-Lymphocytes - metabolism</topic><topic>Ba/F3 cells</topic><topic>Cell Line</topic><topic>DNA - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Interferon-alpha - metabolism</topic><topic>Interferon-α</topic><topic>Interleukin-3 - metabolism</topic><topic>Interleukin-3 - pharmacology</topic><topic>Janus Kinase 2</topic><topic>Mice</topic><topic>Milk Proteins</topic><topic>Protein Binding</topic><topic>Protein-Tyrosine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins</topic><topic>Signal Transduction</topic><topic>STAT5</topic><topic>STAT5 Transcription Factor</topic><topic>Trans-Activators - metabolism</topic><topic>Trans-Activators - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jaster, Robert</creatorcontrib><creatorcontrib>Tschirch, Edda</creatorcontrib><creatorcontrib>Bittorf, Thomas</creatorcontrib><creatorcontrib>Brock, Josef</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jaster, Robert</au><au>Tschirch, Edda</au><au>Bittorf, Thomas</au><au>Brock, Josef</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of STAT5 in Interferon-α Signal Transduction in Ba/F3 Cells</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>1999-05-01</date><risdate>1999</risdate><volume>11</volume><issue>5</issue><spage>331</spage><epage>335</epage><pages>331-335</pages><issn>0898-6568</issn><eissn>1873-3913</eissn><abstract>In interferon-α (IFN-α) signalling, the essential role of the transcription factors STAT1 and STAT2 is well established. In contrast, the involvement of other STAT proteins, including STAT5, is much less well understood. Here we show that, in IFN-α-responsive Ba/F3 cells, this cytokine stimulates the DNA-binding of STAT5A and B but that IL-3 is a much more potent activator of both STAT5 isoforms. A stably expressed dominant-negative mutant of JAK2 suppressed the IL-3- but not the IFN-α-dependent DNA binding of STAT5, suggesting independent mechanisms of its activation. Northern blots revealed that IL-3 strongly induced the expression of two STAT5-regulated genes,
pim-1 and
oncostatin-M, whereas IFN-α had a weak stimulatory effect on
pim-1 expression only. In summary, our results suggest that, despite the capability of IFN-α to stimulate DNA binding of STAT5, this transcription factor does not play a pivotal role in IFN-α signalling in Ba/F3 cells.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>10376805</pmid><doi>10.1016/S0898-6568(98)00049-7</doi><tpages>5</tpages></addata></record> |
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| ispartof | Cellular signalling, 1999-05, Vol.11 (5), p.331-335 |
| issn | 0898-6568 1873-3913 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals B-Lymphocytes - cytology B-Lymphocytes - drug effects B-Lymphocytes - metabolism Ba/F3 cells Cell Line DNA - metabolism DNA-Binding Proteins - metabolism DNA-Binding Proteins - physiology Interferon-alpha - metabolism Interferon-α Interleukin-3 - metabolism Interleukin-3 - pharmacology Janus Kinase 2 Mice Milk Proteins Protein Binding Protein-Tyrosine Kinases - metabolism Proto-Oncogene Proteins Signal Transduction STAT5 STAT5 Transcription Factor Trans-Activators - metabolism Trans-Activators - physiology |
| title | Role of STAT5 in Interferon-α Signal Transduction in Ba/F3 Cells |
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