Nuclear run-on assays: Assessing transcription by measuring density of engaged RNA polymerases

This chapter describes the use of nuclear run-on assays as a direct measure of the density of elongating RNA polymerases. Assuming a relatively constant rate of elongation, this density provides a measure of transcription at the moment of nuclei isolation. Transcriptionally engaged RNA polymerases i...

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Veröffentlicht in:	Methods in Enzymology 1999, Vol.304, p.351-362
Hauptverfasser:	Hirayoshi, Kazunori, Lis, John T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Fractionation - methods Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cells, Cultured Centrifugation, Density Gradient - methods DNA-Directed RNA Polymerases - analysis DNA-Directed RNA Polymerases - metabolism Drosophila Indicators and Reagents Kinetics Mammals Saccharomyces cerevisiae - genetics Transcription, Genetic
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