Evolutionary Differences in Glycosaminoglycan Fine Structure Detected by Quantitative Glycan Reductive Isotope Labeling

To facilitate qualitative and quantitative analysis of glycosaminoglycans, we tagged the reducing end of lyase-generated disaccharides with aniline-containing stable isotopes (12C6 and 13C6). Because different isotope tags have no effect on chromatographic retention times but can be discriminated by...

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Veröffentlicht in:	The Journal of biological chemistry 2008-11, Vol.283 (48), p.33674-33684
Hauptverfasser:	Lawrence, Roger, Olson, Sara K., Steele, Robert E., Wang, Lianchun, Warrior, Rahul, Cummings, Richard D., Esko, Jeffrey D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Caenorhabditis elegans - chemistry Carbohydrate Conformation Carbon Isotopes - chemistry CHO Cells Chromatography, Liquid - methods Cricetinae Cricetulus Drosophila melanogaster Evolution, Molecular Glycobiology and Extracellular Matrices Glycosaminoglycans - chemistry Hydra - chemistry Isotope Labeling - methods Mass Spectrometry - methods Mice
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