Development and validation of a rapid, sensitive liquid chromatography–tandem mass spectrometry method using electrospray ionization for quantitation of centchroman in rat plasma and its application to preclinical pharmacokinetic study

A highly sensitive and specific HPLC–ESI-MS/MS method has been developed and validated for the estimation of centchroman with 100 μL rat plasma using tamoxifen as an internal standard (IS). The assay procedure involved a single-step, liquid–liquid extraction of centchroman and IS from plasma with 2....

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Veröffentlicht in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-12, Vol.876 (1), p.1-7
Hauptverfasser: Singh, Sheelendra Pratap, Singh, Ravi Shankar Prasad, Wahajuddin, Jain, Girish Kumar
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container_title Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
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creator Singh, Sheelendra Pratap
Singh, Ravi Shankar Prasad
Wahajuddin
Jain, Girish Kumar
description A highly sensitive and specific HPLC–ESI-MS/MS method has been developed and validated for the estimation of centchroman with 100 μL rat plasma using tamoxifen as an internal standard (IS). The assay procedure involved a single-step, liquid–liquid extraction of centchroman and IS from plasma with 2.5% (v/v) isopropanol in n-hexane, which yielded consistent recoveries of 109.5 and 107.8% for centchroman and IS in rat plasma, respectively. The total chromatographic run time was 3.8 min. Peaks were resolved using 0.01 M ammonium acetate (pH 4.5):acetonitrile (10:90, v/v) mobile phase on a Supelco Discovery C 18 column. Specificity and matrix effect on ionization was determined and found that method was specific and there was no significant matrix effect. Linearity range was found to be 0.5–100.0 ng/mL with a correlation coefficient ( r) of 0.9959 or better. The intra- and inter-day assay precision ranged from 3.3 to 9.0% and 5.5 to 6.8%, respectively, and intra- and inter-day assay accuracy was between 93.4–107.1% and 96.2–104.2%, respectively. Stability of centchroman in rat plasma was >89.0% in the battery of stability studies viz., bench-top, auto-sampler, freeze/thaw cycles and 30 days storage in a freezer at −80 °C. The assay was successfully applied to determine the pharmacokinetic parameters in Sprague– Dawley rats after an oral administration of centchroman at 20 mg/kg. As a result, the plasma half-life was 29.4 ± 2.3 h and the AUC (0–∞) was 7345.1 ± 21.9 ng h/mL. The maximum plasma concentration ( C max) 117.5 ± 15.7 ng/mL was achieved at 9.0 ± 8.6 h ( t max).
doi_str_mv 10.1016/j.jchromb.2008.09.039
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The assay procedure involved a single-step, liquid–liquid extraction of centchroman and IS from plasma with 2.5% (v/v) isopropanol in n-hexane, which yielded consistent recoveries of 109.5 and 107.8% for centchroman and IS in rat plasma, respectively. The total chromatographic run time was 3.8 min. Peaks were resolved using 0.01 M ammonium acetate (pH 4.5):acetonitrile (10:90, v/v) mobile phase on a Supelco Discovery C 18 column. Specificity and matrix effect on ionization was determined and found that method was specific and there was no significant matrix effect. Linearity range was found to be 0.5–100.0 ng/mL with a correlation coefficient ( r) of 0.9959 or better. The intra- and inter-day assay precision ranged from 3.3 to 9.0% and 5.5 to 6.8%, respectively, and intra- and inter-day assay accuracy was between 93.4–107.1% and 96.2–104.2%, respectively. Stability of centchroman in rat plasma was &gt;89.0% in the battery of stability studies viz., bench-top, auto-sampler, freeze/thaw cycles and 30 days storage in a freezer at −80 °C. The assay was successfully applied to determine the pharmacokinetic parameters in Sprague– Dawley rats after an oral administration of centchroman at 20 mg/kg. As a result, the plasma half-life was 29.4 ± 2.3 h and the AUC (0–∞) was 7345.1 ± 21.9 ng h/mL. 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B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A highly sensitive and specific HPLC–ESI-MS/MS method has been developed and validated for the estimation of centchroman with 100 μL rat plasma using tamoxifen as an internal standard (IS). The assay procedure involved a single-step, liquid–liquid extraction of centchroman and IS from plasma with 2.5% (v/v) isopropanol in n-hexane, which yielded consistent recoveries of 109.5 and 107.8% for centchroman and IS in rat plasma, respectively. The total chromatographic run time was 3.8 min. Peaks were resolved using 0.01 M ammonium acetate (pH 4.5):acetonitrile (10:90, v/v) mobile phase on a Supelco Discovery C 18 column. Specificity and matrix effect on ionization was determined and found that method was specific and there was no significant matrix effect. Linearity range was found to be 0.5–100.0 ng/mL with a correlation coefficient ( r) of 0.9959 or better. The intra- and inter-day assay precision ranged from 3.3 to 9.0% and 5.5 to 6.8%, respectively, and intra- and inter-day assay accuracy was between 93.4–107.1% and 96.2–104.2%, respectively. Stability of centchroman in rat plasma was &gt;89.0% in the battery of stability studies viz., bench-top, auto-sampler, freeze/thaw cycles and 30 days storage in a freezer at −80 °C. The assay was successfully applied to determine the pharmacokinetic parameters in Sprague– Dawley rats after an oral administration of centchroman at 20 mg/kg. As a result, the plasma half-life was 29.4 ± 2.3 h and the AUC (0–∞) was 7345.1 ± 21.9 ng h/mL. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>876</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A highly sensitive and specific HPLC–ESI-MS/MS method has been developed and validated for the estimation of centchroman with 100 μL rat plasma using tamoxifen as an internal standard (IS). The assay procedure involved a single-step, liquid–liquid extraction of centchroman and IS from plasma with 2.5% (v/v) isopropanol in n-hexane, which yielded consistent recoveries of 109.5 and 107.8% for centchroman and IS in rat plasma, respectively. The total chromatographic run time was 3.8 min. Peaks were resolved using 0.01 M ammonium acetate (pH 4.5):acetonitrile (10:90, v/v) mobile phase on a Supelco Discovery C 18 column. Specificity and matrix effect on ionization was determined and found that method was specific and there was no significant matrix effect. Linearity range was found to be 0.5–100.0 ng/mL with a correlation coefficient ( r) of 0.9959 or better. The intra- and inter-day assay precision ranged from 3.3 to 9.0% and 5.5 to 6.8%, respectively, and intra- and inter-day assay accuracy was between 93.4–107.1% and 96.2–104.2%, respectively. Stability of centchroman in rat plasma was &gt;89.0% in the battery of stability studies viz., bench-top, auto-sampler, freeze/thaw cycles and 30 days storage in a freezer at −80 °C. The assay was successfully applied to determine the pharmacokinetic parameters in Sprague– Dawley rats after an oral administration of centchroman at 20 mg/kg. As a result, the plasma half-life was 29.4 ± 2.3 h and the AUC (0–∞) was 7345.1 ± 21.9 ng h/mL. The maximum plasma concentration ( C max) 117.5 ± 15.7 ng/mL was achieved at 9.0 ± 8.6 h ( t max).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19004674</pmid><doi>10.1016/j.jchromb.2008.09.039</doi><tpages>7</tpages></addata></record>
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subjects Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Centchroman
Centchroman - blood
Centchroman - pharmacokinetics
Chromatography, High Pressure Liquid - methods
Drug Stability
Female
Fundamental and applied biological sciences. Psychology
General pharmacology
LC–MS/MS
Medical sciences
Pharmacokinetics
Pharmacology. Drug treatments
Rat plasma
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
Validation
title Development and validation of a rapid, sensitive liquid chromatography–tandem mass spectrometry method using electrospray ionization for quantitation of centchroman in rat plasma and its application to preclinical pharmacokinetic study
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