Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in ∼20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from ∼95% of mul...

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Veröffentlicht in:Nature genetics 2008-12, Vol.40 (12), p.1413-1415
Hauptverfasser: Blencowe, Benjamin J, Pan, Qun, Shai, Ofer, Lee, Leo J, Frey, Brendan J
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container_title Nature genetics
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creator Blencowe, Benjamin J
Pan, Qun
Shai, Ofer
Lee, Leo J
Frey, Brendan J
description We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in ∼20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from ∼95% of multiexon genes undergo alternative splicing and that there are ∼100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.
doi_str_mv 10.1038/ng.259
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subjects Agriculture
Alternative Splicing
Animal Genetics and Genomics
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brain
brief-communication
Cancer Research
DNA microarrays
DNA sequencing
Estimates
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling
Gene Function
Genetic engineering
Genetics of eukaryotes. Biological and molecular evolution
Human Genetics
Humans
Medical research
Messenger RNA
Methods
Nucleotide sequencing
Physiological aspects
RNA Splice Sites
RNA, Messenger
Sequence Analysis, RNA - methods
Studies
Tissues
title Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing
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