Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid
Expression patterns of candidate genes with important functions in animal metabolism can help to identify potential molecular markers for cattle production traits. Reverse transcription followed by polymerase chain reaction is a method for rapid and accurate mRNA quantification. However, for exact c...
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| Veröffentlicht in: | Journal of applied genetics 2008-01, Vol.49 (4), p.367-372 |
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| container_title | Journal of applied genetics |
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| creator | Lisowski, Paweł Pierzchała, Mariusz Gościk, Joanna Pareek, Chandra S. Zwierzchowski, Lech |
| description | Expression patterns of candidate genes with important functions in animal metabolism can help to identify potential molecular markers for cattle production traits. Reverse transcription followed by polymerase chain reaction is a method for rapid and accurate mRNA quantification. However, for exact comparison of mRNA quantity in various samples or tissues, it is important to choose appropriate reference genes. In cattle, little information is available on the expression stability of housekeeping genes (HKGs). The aim of the present study is to develop a set of reference genes that can be used for normalization of concentrations of mRNAs of genes expressed in the bovine liver, kidney, pituitary and thyroid. The study was performed on 6-, 9-, and 12-month-old bulls of dairy and meat cattle breeds. Six HKGs were investigated:
ACTB, GAPDH, HPRTI, SDHA, TBP
, and
YWHAZ
. The most stably expressed potential reference HKGs differed among tissues/organs examined:
ACTB, TBP, YWHAZ, GAPDH, HPR TI
, and
SDHA
in the liver;
GAPDH
and
YWHAZ
in the kidney;
GAPDH
and
SDHA
in the pituitary; and
TBP
and
HPRTI
in the thyroid. The results showed that the use of a single gene fornormalization may lead to relatively large errors, so it is important to use multiple control genes based on a survey of potential reference genes applied to representative samples from specific experimental conditions. |
| doi_str_mv | 10.1007/BF03195635 |
| format | Article |
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ACTB, GAPDH, HPRTI, SDHA, TBP
, and
YWHAZ
. The most stably expressed potential reference HKGs differed among tissues/organs examined:
ACTB, TBP, YWHAZ, GAPDH, HPR TI
, and
SDHA
in the liver;
GAPDH
and
YWHAZ
in the kidney;
GAPDH
and
SDHA
in the pituitary; and
TBP
and
HPRTI
in the thyroid. The results showed that the use of a single gene fornormalization may lead to relatively large errors, so it is important to use multiple control genes based on a survey of potential reference genes applied to representative samples from specific experimental conditions.</description><identifier>ISSN: 1234-1983</identifier><identifier>EISSN: 2190-3883</identifier><identifier>DOI: 10.1007/BF03195635</identifier><identifier>PMID: 19029684</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Animal Genetics and Genomics ; Animals ; Biomarkers - analysis ; Biomedical and Life Sciences ; Cattle - genetics ; Gene Expression ; Human Genetics ; Kidney - metabolism ; Life Sciences ; Liver - metabolism ; Male ; Microbial Genetics and Genomics ; Origial Article ; Pituitary Gland - metabolism ; Plant Genetics and Genomics ; Polymerase Chain Reaction ; Reference Standards ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Thyroid Gland - metabolism</subject><ispartof>Journal of applied genetics, 2008-01, Vol.49 (4), p.367-372</ispartof><rights>Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-a56ab33d1904eeb42acca272f507e345c4ed49fe649459bff51d6d82e8f88d2c3</citedby><cites>FETCH-LOGICAL-c418t-a56ab33d1904eeb42acca272f507e345c4ed49fe649459bff51d6d82e8f88d2c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/BF03195635$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/BF03195635$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19029684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lisowski, Paweł</creatorcontrib><creatorcontrib>Pierzchała, Mariusz</creatorcontrib><creatorcontrib>Gościk, Joanna</creatorcontrib><creatorcontrib>Pareek, Chandra S.</creatorcontrib><creatorcontrib>Zwierzchowski, Lech</creatorcontrib><title>Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid</title><title>Journal of applied genetics</title><addtitle>J Appl Genet</addtitle><addtitle>J Appl Genet</addtitle><description>Expression patterns of candidate genes with important functions in animal metabolism can help to identify potential molecular markers for cattle production traits. Reverse transcription followed by polymerase chain reaction is a method for rapid and accurate mRNA quantification. However, for exact comparison of mRNA quantity in various samples or tissues, it is important to choose appropriate reference genes. In cattle, little information is available on the expression stability of housekeeping genes (HKGs). The aim of the present study is to develop a set of reference genes that can be used for normalization of concentrations of mRNAs of genes expressed in the bovine liver, kidney, pituitary and thyroid. The study was performed on 6-, 9-, and 12-month-old bulls of dairy and meat cattle breeds. Six HKGs were investigated:
ACTB, GAPDH, HPRTI, SDHA, TBP
, and
YWHAZ
. The most stably expressed potential reference HKGs differed among tissues/organs examined:
ACTB, TBP, YWHAZ, GAPDH, HPR TI
, and
SDHA
in the liver;
GAPDH
and
YWHAZ
in the kidney;
GAPDH
and
SDHA
in the pituitary; and
TBP
and
HPRTI
in the thyroid. The results showed that the use of a single gene fornormalization may lead to relatively large errors, so it is important to use multiple control genes based on a survey of potential reference genes applied to representative samples from specific experimental conditions.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biomarkers - analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Cattle - genetics</subject><subject>Gene Expression</subject><subject>Human Genetics</subject><subject>Kidney - metabolism</subject><subject>Life Sciences</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Microbial Genetics and Genomics</subject><subject>Origial Article</subject><subject>Pituitary Gland - metabolism</subject><subject>Plant Genetics and Genomics</subject><subject>Polymerase Chain Reaction</subject><subject>Reference Standards</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Thyroid Gland - metabolism</subject><issn>1234-1983</issn><issn>2190-3883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxTAQhYMoen1s_AGSlQu1mlfbZKniCwQ3ui5pM9Fob1KT9qL_3lzuBTeCqww53xxmziB0SMk5JaS-uLolnKqy4uUGmjGqSMGl5JtoRhkXBVWS76DdlN4J4VLUbBvtZIapSooZGm4Wup_06ILHweIIFiL4DvAreEjYhojTOBmX6ywvPzF8DRFSWnY4j8c3wG1YuCz0bgHxDH844-H7DA9unNyoYy61Nxn8jsGZfbRldZ_gYP3uoZfbm-fr--Lx6e7h-vKx6ASVY6HLSrecmzypAGgF012nWc1sSWrgouwEGKEsVEKJUrXWltRURjKQVkrDOr6Hjle-QwyfE6SxmbvUQd9rD2FKTaUk41Sof0FGZFWTusrgyQrsYkgpJ9UM0c3zfg0lzfIQze8hMny0dp3aOZhfdJ18Bk5XQMqSf4XYvIcp-hzJX3Y_jH2RwA</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Lisowski, Paweł</creator><creator>Pierzchała, Mariusz</creator><creator>Gościk, Joanna</creator><creator>Pareek, Chandra S.</creator><creator>Zwierzchowski, Lech</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid</title><author>Lisowski, Paweł ; Pierzchała, Mariusz ; Gościk, Joanna ; Pareek, Chandra S. ; Zwierzchowski, Lech</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-a56ab33d1904eeb42acca272f507e345c4ed49fe649459bff51d6d82e8f88d2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biomarkers - analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Cattle - genetics</topic><topic>Gene Expression</topic><topic>Human Genetics</topic><topic>Kidney - metabolism</topic><topic>Life Sciences</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Microbial Genetics and Genomics</topic><topic>Origial Article</topic><topic>Pituitary Gland - metabolism</topic><topic>Plant Genetics and Genomics</topic><topic>Polymerase Chain Reaction</topic><topic>Reference Standards</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Thyroid Gland - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lisowski, Paweł</creatorcontrib><creatorcontrib>Pierzchała, Mariusz</creatorcontrib><creatorcontrib>Gościk, Joanna</creatorcontrib><creatorcontrib>Pareek, Chandra S.</creatorcontrib><creatorcontrib>Zwierzchowski, Lech</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lisowski, Paweł</au><au>Pierzchała, Mariusz</au><au>Gościk, Joanna</au><au>Pareek, Chandra S.</au><au>Zwierzchowski, Lech</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid</atitle><jtitle>Journal of applied genetics</jtitle><stitle>J Appl Genet</stitle><addtitle>J Appl Genet</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>49</volume><issue>4</issue><spage>367</spage><epage>372</epage><pages>367-372</pages><issn>1234-1983</issn><eissn>2190-3883</eissn><abstract>Expression patterns of candidate genes with important functions in animal metabolism can help to identify potential molecular markers for cattle production traits. Reverse transcription followed by polymerase chain reaction is a method for rapid and accurate mRNA quantification. However, for exact comparison of mRNA quantity in various samples or tissues, it is important to choose appropriate reference genes. In cattle, little information is available on the expression stability of housekeeping genes (HKGs). The aim of the present study is to develop a set of reference genes that can be used for normalization of concentrations of mRNAs of genes expressed in the bovine liver, kidney, pituitary and thyroid. The study was performed on 6-, 9-, and 12-month-old bulls of dairy and meat cattle breeds. Six HKGs were investigated:
ACTB, GAPDH, HPRTI, SDHA, TBP
, and
YWHAZ
. The most stably expressed potential reference HKGs differed among tissues/organs examined:
ACTB, TBP, YWHAZ, GAPDH, HPR TI
, and
SDHA
in the liver;
GAPDH
and
YWHAZ
in the kidney;
GAPDH
and
SDHA
in the pituitary; and
TBP
and
HPRTI
in the thyroid. The results showed that the use of a single gene fornormalization may lead to relatively large errors, so it is important to use multiple control genes based on a survey of potential reference genes applied to representative samples from specific experimental conditions.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>19029684</pmid><doi>10.1007/BF03195635</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal Genetics and Genomics Animals Biomarkers - analysis Biomedical and Life Sciences Cattle - genetics Gene Expression Human Genetics Kidney - metabolism Life Sciences Liver - metabolism Male Microbial Genetics and Genomics Origial Article Pituitary Gland - metabolism Plant Genetics and Genomics Polymerase Chain Reaction Reference Standards Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Thyroid Gland - metabolism |
| title | Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid |
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