Response to IL-6 of HPV-18 Cervical Carcinoma Cell Lines
The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls cell type-specific expression of the viral oncoproteins E6 and E7. The HPV-18 URR is active in the cervical carcinoma cell line HeLa but inactive in the hepatoma cell line HepG2. C/EBPß (NF-IL-6) was shown to partici...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1999-06, Vol.258 (2), p.344-354 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Bauknecht, Tobias Randelzhofer, Bettina Schmitt, Beate Ban, Zoltan Hernando, Juan-Jose Bauknecht, Thomas |
| description | The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls cell type-specific expression of the viral oncoproteins E6 and E7. The HPV-18 URR is active in the cervical carcinoma cell line HeLa but inactive in the hepatoma cell line HepG2. C/EBPß (NF-IL-6) was shown to participate as an important regulator in HPV transcription dependent on the cell type. The finding that C/EPBß is critical for HPV-18 URR activity and that C/EPBß is induced by IL-6 offers the opportunity of manipulating HPV activity by specific cytokine treatment. In this report, we show that treatment with IL-6 results in activation of HPV-18 URR activity in HepG2 cells. In contrast, the HPV-18 URR is not inducible by IL-6 in three cervical carcinoma cell lines. In all three cell lines we found decreased expression of the IL-6 receptor compared to the IL-6-responsive HepG2 cells, whereas the level of expression of the signal transduction component gp130 is present in all cells. These results suggest that cervical carcinoma cells may circumvent the IL-6-induced cellular defense mechanism through downregulation of the IL-6-receptor. |
| doi_str_mv | 10.1006/viro.1999.9722 |
| format | Article |
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The HPV-18 URR is active in the cervical carcinoma cell line HeLa but inactive in the hepatoma cell line HepG2. C/EBPß (NF-IL-6) was shown to participate as an important regulator in HPV transcription dependent on the cell type. The finding that C/EPBß is critical for HPV-18 URR activity and that C/EPBß is induced by IL-6 offers the opportunity of manipulating HPV activity by specific cytokine treatment. In this report, we show that treatment with IL-6 results in activation of HPV-18 URR activity in HepG2 cells. In contrast, the HPV-18 URR is not inducible by IL-6 in three cervical carcinoma cell lines. In all three cell lines we found decreased expression of the IL-6 receptor compared to the IL-6-responsive HepG2 cells, whereas the level of expression of the signal transduction component gp130 is present in all cells. 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These results suggest that cervical carcinoma cells may circumvent the IL-6-induced cellular defense mechanism through downregulation of the IL-6-receptor.</description><subject>Antigens, CD - genetics</subject><subject>Cytokine Receptor gp130</subject><subject>Female</subject><subject>Gene Expression Regulation, Viral - drug effects</subject><subject>HeLa Cells</subject><subject>Human papillomavirus 16</subject><subject>Humans</subject><subject>Interleukin-6 - immunology</subject><subject>Interleukin-6 - pharmacology</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomaviridae - immunology</subject><subject>Receptors, Interleukin-6 - genetics</subject><subject>Tumor Cells, Cultured</subject><subject>Uterine Cervical Neoplasms - immunology</subject><subject>Uterine Cervical Neoplasms - virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rp69Sg9eWtN0jYfRynqLhQUWbyGNJ2FSNusSXfBf29K9-BFZA7DDM-8DA9CtwRnBGP2cLTeZURKmUlO6RlaEixZivOCnKMlxgVNmaB0ga5C-MRx5hxfogXBOWMlJ0sk3iHs3RAgGV2yqVOWuF2yfvtIiUgq8EdrdJdU2hs7uF7HVdcltR0gXKOLne4C3Jz6Cm2fn7bVOq1fXzbVY52agpAxJUawpjG5znMuJZe00WBKKLQwmmMQbQushFiEtE2ZF1I0rZTaUKYNxk2-Qvdz7N67rwOEUfU2mPiFHsAdgmJSEMby8l-QcFoIKngEsxk03oXgYaf23vbafyuC1eRUTU7V5FRNTuPB3Sn50PTQ_sJniREQMwDRw9GCV8FYGAy01oMZVevsX9k_3L-Dlg</recordid><startdate>19990605</startdate><enddate>19990605</enddate><creator>Bauknecht, Tobias</creator><creator>Randelzhofer, Bettina</creator><creator>Schmitt, Beate</creator><creator>Ban, Zoltan</creator><creator>Hernando, Juan-Jose</creator><creator>Bauknecht, Thomas</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19990605</creationdate><title>Response to IL-6 of HPV-18 Cervical Carcinoma Cell Lines</title><author>Bauknecht, Tobias ; 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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | Antigens, CD - genetics Cytokine Receptor gp130 Female Gene Expression Regulation, Viral - drug effects HeLa Cells Human papillomavirus 16 Humans Interleukin-6 - immunology Interleukin-6 - pharmacology Membrane Glycoproteins - genetics Papillomaviridae - genetics Papillomaviridae - immunology Receptors, Interleukin-6 - genetics Tumor Cells, Cultured Uterine Cervical Neoplasms - immunology Uterine Cervical Neoplasms - virology |
| title | Response to IL-6 of HPV-18 Cervical Carcinoma Cell Lines |
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