A novel recognition motif of human NKT antigen receptor for a glycolipid ligand
Murine NKT cells can recognize α-galactosylceramide (α-GalCer) in the context of a class Ib CD1d molecule. Here we show that α-GalCer can selectively activate freshly isolated human Vα24+Vβ11+ cells, functionally defining the human NKT cells. The naive human NKT cell repertoire consisted of cells ex...
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| Veröffentlicht in: | International immunology 1999-06, Vol.11 (6), p.881-887 |
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| container_title | International immunology |
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| creator | Kawano, Tetsu Tanaka, Yujiro Shimizu, Eiko Kaneko, Yoshikatsu Kamata, Noriaki Sato, Hiroshi Osada, Hisao Sekiya, Soei Nakayama, Toshinori Taniguchi, Masaru |
| description | Murine NKT cells can recognize α-galactosylceramide (α-GalCer) in the context of a class Ib CD1d molecule. Here we show that α-GalCer can selectively activate freshly isolated human Vα24+Vβ11+ cells, functionally defining the human NKT cells. The naive human NKT cell repertoire consisted of cells expressing an invariant Vα24JαQ chain and a diverse array of β chains derived from a single Vβ11 gene segment. Stimulation with α-GalCer expanded a polyclonal subset of the human NKT cell repertoire carrying a novel complementarity-determining region (CDR) 3β consensus motif that may directly interact with the sugar moiety of α-GalCer. Our data suggest that certain redundancy is allowed for CDR3β of NKT antigen receptor to interact with the ligand and provide a first clue to understand the novel protein–carbohydrate interaction mechanisms. |
| doi_str_mv | 10.1093/intimm/11.6.881 |
| format | Article |
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Immunol</addtitle><description>Murine NKT cells can recognize α-galactosylceramide (α-GalCer) in the context of a class Ib CD1d molecule. Here we show that α-GalCer can selectively activate freshly isolated human Vα24+Vβ11+ cells, functionally defining the human NKT cells. The naive human NKT cell repertoire consisted of cells expressing an invariant Vα24JαQ chain and a diverse array of β chains derived from a single Vβ11 gene segment. Stimulation with α-GalCer expanded a polyclonal subset of the human NKT cell repertoire carrying a novel complementarity-determining region (CDR) 3β consensus motif that may directly interact with the sugar moiety of α-GalCer. 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| identifier | ISSN: 0953-8178 |
| ispartof | International immunology, 1999-06, Vol.11 (6), p.881-887 |
| issn | 0953-8178 1460-2377 1460-2377 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adult Animals Antigens, CD1 - immunology Antigens, CD1d APC antigen-presenting cell Binding Sites - immunology carbohydrate-binding proteins CDR3 complementarity-determining region 3 complementarity-determining region CRD carbohydrate-recognition domain Fetal Blood - cytology Fetal Blood - immunology Galactosylceramides - immunology Galactosylceramides - metabolism Humans Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Ligands Lymphocyte Activation - immunology Mice NKT antigen receptor PE phycoerythrin protein–sugar interaction Receptors, Antigen, T-Cell, alpha-beta - immunology Receptors, Antigen, T-Cell, alpha-beta - metabolism repertoire selection T-Lymphocyte Subsets - immunology α-GalCer α-galactosylceramide |
| title | A novel recognition motif of human NKT antigen receptor for a glycolipid ligand |
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