Expression of human plasminogen in Drosophila Schneider S2 cells

The cDNA that encodes full-length human plasminogen (Glu1-hPg) has been expressed in Drosophila Schneider S2 cells under the influence of the Drosophila BiP protein signal sequence, which allowed the protein to be secreted into the medium. A procedure was devised for clonal selection of high-express...

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Veröffentlicht in:	Protein expression and purification 1999-06, Vol.16 (1), p.136-143
Hauptverfasser:	Nilsen, S L, Castellino, F J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA Primers - genetics Drosophila - genetics Drug Stability Gene Expression Humans Kinetics Plasmids - genetics Plasminogen - chemistry Plasminogen - genetics Plasminogen - metabolism Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Thermodynamics Transfection
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